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DAB2IP Antibody

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Western Blot: DAB2IP Antibody [NBP1-30081] - Detection of Mouse DAB2IP by Western Blot. Samples: Whole cell lysate (50 ug) from TCMK-1, Renca, 4T1, and CT26.WT cells. Antibodies: Affinity purified rabbit anti-DAB2IP antibody NBP1-30081 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Biological Strategies: Western Blot: DAB2IP Antibody [NBP1-30081] - Detection of Mouse DAB2IP by Western Blot. Samples: Whole cell lysate (50 ug) from TCMK-1, Renca, 4T1, and CT26.WT cells. Antibodies: Affinity ...read more
Independent Antibodies: Western Blot: DAB2IP Antibody [NBP1-30081] - Whole cell lysate from HeLa cells. DAB2IP was also IPed by rabbit anti-DAB2IP antibody NBP1-30082. For blotting immunoprecipitated DAB2IP, the ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.2 mg/ml
Validated by:
 

Independent Antibodies

  

Biological Strategies

      

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DAB2IP Antibody Summary

Immunogen
Antigen maps to a region between residue 850 and 900 of human disabled homolog 2 interacting protein using the numbering given in entry CAQ10385.1
Predicted Species
Rat (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
DAB2IP
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Immunoprecipitation 2-5 ug/mg lysate
  • Western Blot 1:2000-1:10000
Theoretical MW
132 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publication using NBP1-30081.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
TBS and 0.1% BSA
Preservative
0.09% Sodium Azide
Concentration
0.2 mg/ml
Purity
Immunogen affinity purified

Alternate Names for DAB2IP Antibody

  • AIP1FLJ39072
  • ASK-interacting protein 1
  • DAB2 interacting protein
  • DAB2 interaction protein
  • DAB2-interacting protein
  • DIP1/2
  • disabled homolog 2-interacting protein
  • DOC-2/DAB2 interactive protein
  • KIAA1743AF9Q34ASK1-interacting protein 1
  • nGAP-like protein

Background

DAB2-interacting protein (DAB2IP) functions as a Ras GTPase-activating protein. Through its interaction with MAP3K5, it disrupts the association of MAP3K5 with the inhibitory 14-3-3 complex. DAB2IP also Mediates TNF/TRAF2-induced MAP3K5-JNK activation, while it inhibits CHUK-NF-kappa-B signaling. DAB2IP may act as a tumor suppressor gene.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Western blot results for A172, HeLa and MCF 10A cell line lysates. A PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human DAB2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8064) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for DAB2 at approximately 96 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
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Immunocytochemistry/Immunofluorescence: PDCD6 Antibody [NBP2-94061] - Analysis of HeLa cells using PDCD6 . Blue: DAPI for nuclear staining.
Immunohistochemistry-Paraffin: PDCD6 Antibody [NBP2-94061] - Paraffin-embedded rat kidney using PDCD6 .
Western Blot: PDCD6 Antibody [NBP2-94061] - Analysis of extracts of various cell lines, using PDCD6 at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit . Exposure time: 90s.
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Flow Cytometry: Destrin Antibody (2F7) [NBP2-00527] - Analysis of Jurkat cells, using anti-Destrin antibody, (Red), compared to a nonspecific negative control antibody (Blue).
Flow Cytometry: Destrin Antibody (2F7) [NBP2-00527] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-Destrin antibody, and then analyzed by flow cytometry.
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Flow Cytometry: ASK1 Antibody (SU36-06) [NBP2-67481] - Analysis of Hela cells with ASK1 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.
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Flow Cytometry: TSG101 Antibody (4A10) [NB200-112] - An intracellular stain was performed on Hek293 cells with TSG101 Antibody [4A10] NB200-112PE (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Phycoerythrin.
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Western Blot: VPS4A Antibody [NBP1-54618] - Reccomended Titration: 0.2 - 1 ug/ml ELISA Titer: 1:312500 Positive Control: Human Muscle
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Glutaminyl-peptide
Cyclotransferase/QPCT ...
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(1 Publication)
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240)
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Flow Cytometry: p53 Antibody (PAb 240) [NB200-103] - An intracellular stain was performed on A549 cells with p53 (PAb240) NB200-103AF488 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 10 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.
Flow (Intracellular): p53 Antibody (PAb 240) [NB200-103] - An intracellular stain was performed on HeLa cells with p53 (PAb240) NB200-103 (blue) and a matched isotype control NBP2-27287 (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature, followed by APC-conjugated anti-mouse IgG secondary antibody F0101B.
(3 Reviews)
(33 Publications)
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Species: Hu
Applications: IHC, IHC-P, IP, WB
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Flow Cytometry: MKK4/MEK4 Antibody (OTI8A8) [NBP1-47839] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-MEK4 antibody, and then analyzed by flow cytometry.
Immunocytochemistry/Immunofluorescence: MKK4/MEK4 Antibody (OTI8A8) [NBP1-47839] - Staining of HepG2 cells using anti-MEK4 mouse monoclonal antibody.
Immunocytochemistry/Immunofluorescence: MKK4/MEK4 Antibody (OTI8A8) [NBP1-47839] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY MEK4.
(1 Review)
Species: Ca, Hu, Mu, Rt
Applications: Flow, ICC/IF, IHC, IHC-P, IP, WB
DAB2IP Antibody
NBP1-30081
DAB2IP Antibody
Species: Hu, Mu, Rt
Applications: WB, IP

Publications for DAB2IP Antibody (NBP1-30081)(1)


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Bioinformatics

Gene Symbol DAB2IP
Entrez
Uniprot