Cytokeratin 7 Antibody

Knockout Validated: Cytokeratin 7 Antibody [NBP2-16093] - Wild-type (WT) and Cytokeratin 7 knockout (KO) HeLa cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Cytokeratin 7 antibody [N2C2]. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Western Blot: Cytokeratin 7 Antibody [NBP2-16093] - Sample (30 ug of whole cell lysate) A: HeLa 10% SDS PAGE gel, diluted at 1:10000.

Immunocytochemistry/Immunofluorescence: Cytokeratin 7 Antibody [NBP2-16093] - Immunofluorescence analysis of methanol-fixed HeLa, using antibody at 1:500 dilution.

Immunohistochemistry-Paraffin: Cytokeratin 7 Antibody [NBP2-16093] - Immunohistochemical analysis of paraffin-embedded SkHep1 xenograft, using antibody at 1:500 dilution.

Product Discontinued

Cytokeratin 7 Antibody Summary

• Immunogen: Recombinant protein encompassing a sequence within the center region of human Cytokeratin 7. The exact sequence is proprietary.
• Localization: Cytoplasm
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: KRT7
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 10 - 1:500
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Knockout Validated
  • Western Blot 1:5000-1:50000
• Theoretical MW: 51 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (88%), Rat (88%), Bovine (85%).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: 0.1M Tris (pH 7), 0.1M Glycine, 20% Glycerol
• Preservative: 0.01% Thimerosal
• Purity: Immunogen affinity purified

Alternate Names for Cytokeratin 7 Antibody

• CK7
• CK-7
• Cytokeratin 7
• cytokeratin-7
• K2C7
• K7
• K7keratin, 55K type II cytoskeletal
• keratin 7
• keratin, type II cytoskeletal 7
• keratin-7
• MGC129731
• MGC3625
• sarcolectin
• SCLkeratin, simple epithelial type I, K7
• type II mesothelial keratin K7
• Type-II keratin Kb7

Background

The protein encoded by this gene is a member of the keratin gene family. The type II cytokeratins consist of basic or neutral proteins which are arranged in pairs of heterotypic keratin chains coexpressed during differentiation of simple and stratified epithelial tissues. This type II cytokeratin is specifically expressed in the simple epithelia lining the cavities of the internal organs and in the gland ducts and blood vessels. The genes encoding the type II cytokeratins are clustered in a region of chromosome 12q12-q13. Alternative splicing may result in several transcript variants; however, not all variants have been fully described. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Bioinformatics

• Gene Symbol: KRT7
• Uniprot:
  • P08729(Human)