Cytokeratin 7/17 Antibody (C-46) [PE]

Flow Cytometry: Cytokeratin 7/17 Antibody (C-46) [PE] [NB500-352PE] - An intracellular stain was performed on HeLa cells with Cytokeratin 7/17 antibody (C-46) NB500-352PE (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Phycoerythrin.

Flow Cytometry: Cytokeratin 7/17 Antibody (C-46) [PE] [NB500-352PE] - An intracellular stain was performed on HeLa cells with Cytokeratin 7/17 (C-46) antibody NB500-352PE (blue) and a matched isotype control NBP2-27287PE (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Phycoerythrin.

• Reactivity: Hu, Po, Bv, Mu(-), Rb(-), Rt(-)
• Applications: WB, Flow, ICC/IF, IHC, IP, CyTOF-ready
• Clone: C-46
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: PE

Cytokeratin 7/17 Antibody (C-46) [PE] Summary

• Description: This conjugate is made on demand and is not held in inventory. Each order represents a unique lot. All conjugates are generated using validated conjugation protocols and meet strict degree of labeling (F/P) specifications; additional information is available upon request. The antibody concentration is provided on the individual product label.
  
  For specialized conjugation needs, like large quantities, specific concentrations, and defined F/P ratios, bulk and custom antibody conjugation services are available.
• Immunogen: Cytoskeleton preparation of HeLa human cervix carcinoma cell line.
• Specificity: The antibody C-46 reacts with Cytokeratin peptides 7 and 17 (54 and 46 kDa). Cytokeratins are a member of intermediate filaments subfamily represented in epithelial tissues.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: KRT7
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow (Intracellular)
  • Flow Cytometry
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Immunoprecipitation
  • Western Blot
• Application Notes: Recommended applications are based on validated applications from the unconjugated base product NB500-352. This conjugated antibody is not kept in inventory and is made to order using validated conjugation protocols.

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: PBS
• Preservative: 0.05% Sodium Azide
• Purity: Protein A purified

Alternate Names for Cytokeratin 7/17 Antibody (C-46) [PE]

• CK7
• CK-7
• cytokeratin 7
• cytokeratin-7
• K2C7
• K7keratin, 55K type II cytoskeletal
• keratin 7
• keratin, type II cytoskeletal 7
• keratin-7
• MGC129731
• MGC3625
• sarcolectin
• SCLkeratin, simple epithelial type I, K7
• type II mesothelial keratin K7
• Type-II keratin Kb7

Background

The protein encoded by the Cytokeratin 7 / 17 gene is a member of the keratin gene family. The type II cytokeratins consist of basic or neutral proteins which are arranged in pairs of heterotypic keratin chains coexpressed during differentiation of simple and stratified epithelial tissues. This type II cytokeratin is specifically expressed in the simple epithelia lining the cavities of the internal organs and in the gland ducts and blood vessels. The genes encoding the type II cytokeratins are clustered in a region of chromosome 12q12-q13. Alternative splicing may result in several transcript variants; however, not all variants have been fully described. (provided by RefSeq)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Cytokeratin 7/17 Antibody (NB500-352PE)(1)

Publication using NB500-352PE

• Cheng, K S, Pan, R Et al. ALICE: a hybrid AI paradigm with enhanced connectivity and cybersecurity for a serendipitous encounter with circulating hybrid cells. Theranostics 2020-10-13 [PMID: 33042268] (WB, Human)

Bioinformatics

• Gene Symbol: KRT7
• Entrez:
  • 3855(Human)
  • 110310(Mouse)
  • 300242(Rat)