Cytokeratin 19 Antibody (4F12G9)

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Western Blot: Cytokeratin 19 Antibody (4F12G9) [NBP1-47429] - Analysis using KRT19 mouse mAb against truncated KRT19-His recombinant protein (1) and full-length KRT19(aa1-400)-hIgGFc transfected CHO-K1 cell lysate(2).

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA
Clone
4F12G9
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

Cytokeratin 19 Antibody (4F12G9) Summary

Immunogen
Purified recombinant fragment of Cytokeratin 19(aa80-400) expressed in E. Coli strain.
Marker
Epithelial Cell Marker
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
KRT19
Purity
Unpurified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Western Blot 1:500 - 1:2000
  • ELISA 1:10000
Theoretical MW
44 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
Ascites
Preservative
0.03% Sodium Azide
Purity
Unpurified

Alternate Names for Cytokeratin 19 Antibody (4F12G9)

  • CK19
  • CK-19
  • Cytokeratin 19
  • Cytokeratin-19,40-kDa keratin intermediate filament
  • EndoC
  • K19
  • K19cytokeratin-19
  • K1CS
  • keratin 19
  • keratin, type I cytoskeletal 19
  • keratin, type I, 40-kd
  • keratin-19
  • Krt19
  • MGC15366

Background

Keratins are a family of intermediate filament proteins that assemble into filaments through forming heterodimers of one type I keratin (keratins 9 to 23) and one type II keratin (keratins 1-8). The two keratin types share only 30% sequence homology. (2-6) Keratins demonstrate tissue- and differentiation-specific expression profiles. Cytokeratin 19 is the smallest human keratin, is found in many simple and some non-keratinizing stratified squamous epithelia, and is often co-expressed with keratin 7. (7,8) Cytokeratin 19 has been implicated as a marker for stem cells in some tissues, such as hair follicles in the skin. Monoclonal antibodies to cytokeratin proteins can be useful markers for tumor identification and classification. The degree of cytokeratin 19 positivity in breast cancer distinguishes malignant from benign tumors. Cytokeratin 19 is also associated with malignant transformation in esophageal and pancreatic cancers. (9,10)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol KRT19
Entrez