Cytokeratin 17 Antibody - BSA Free

Immunohistochemistry-Paraffin: Cytokeratin 17 Antibody [NBP1-32824] - Cytokeratin 17 antibody detects Cytokeratin 17 protein at cytoplasm in human cervical metaplasia (left) and carcinoma (right) by immunohistochemical analysis.Sample: Paraffin-embedded human cervix. Antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Western Blot: Cytokeratin 17 Antibody [NBP1-32824] - Sample (50 ug of whole cell lysate) A: Rat bladder 10% SDS PAGE; antibody diluted at 1:10000.

Immunocytochemistry/Immunofluorescence: Cytokeratin 17 Antibody [NBP1-32824] - Paraformaldehyde-fixed HeLa, using Cytokeratin 17 antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with an alpha Tubulin antibody (Red) at 1:2500.

Immunohistochemistry-Paraffin: Cytokeratin 17 Antibody [NBP1-32824] - Paraffin-embedded N87 xenograft, using antibody at 1:100 dilution.

Immunohistochemistry-Paraffin: Cytokeratin 17 Antibody [NBP1-32824] - Cytokeratin 17 antibody detects Cytokeratin 17 protein at cytoplasm on mouse esophagus by immunohistochemical analysis. Sample: Paraffin-embedded mouse esophagus.Cytokeratin 17 antibody diluted at 1:500. Antigen Retrieval: Trilogy (TM) (EDTA based, pH 8.0) buffer, 15min

Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Cytokeratin 17 (NBP1-32824) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

Cytokeratin 17 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit Cytokeratin 17 Antibody - BSA Free (NBP1-32824) is a polyclonal antibody validated for use in IHC, WB and ICC/IF. Anti-Cytokeratin 17 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant protein encompassing a sequence within the center region of human Cytokeratin 17. The exact sequence is proprietary.
• Localization: Cytoplasm
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: KRT17
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 10 - 1:500
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:5000-1:20000

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS, 20% Glycerol
• Preservative: 0.025% Proclin 300
• Purity: Antigen Affinity-purified

Alternate Names for Cytokeratin 17 Antibody - BSA Free

• 39.1
• CK-17
• Cytokeratin 17
• cytokeratin-17
• K17
• keratin 17
• keratin 17, type I
• keratin, type I cytoskeletal 17
• keratin-17
• PC
• PC2
• PCHC1

Background

Cytokeratins, a group of at least 29 different proteins, are characteristic of epithelial and trichocytic cells. Cytokeratins 4, 5, 6 and 8 are members of the type II neutral-to-basic subfamily. Cytokeratin peptide 4 (59 kDa) is the secondary type II keratin expressed in non cornified stratified squamous epithelia. Cytokeratin peptide 5 (58 kDa) is the primary type II keratin in stratified epithelia, while cytokeratin type 8 (52 kDa) is a major type II keratin in simple epithelia. Cytokeratin 6 (56 kDa) is a "hyperproliferation" cytokeratin expressed in tissues with natural or pathological high turnover. Cytokeratins 10, 13 and 18 are members of the type I acidic subfamily. Cytokeratin peptide 10 (56 kDa) is the secondary type I keratin expressed in cornified epithelia. Cytokeratin 13 (54 kDa) is the secondary type I keratin expressed in non-cornified stratified squamous epithelia. Cytokeratin 18 (45 kDa) is the primary type I keratin expressed in simple epithelial cells. Cytokeratin is a heterotetramer of two type I and two type II keratins. Keratin 1 is generally associated with keratin 10. Defects in Cytokeratin are a cause of epidermolytic hyperkeratosis, also known as bullous congenital ichthyosiform erythroderma (BIE), which is a hereditary skin disorder characterized by blistering and a marked thickening of the stratum corneum. Defects in Cytokeratin are also the cause of Curth Macklin type ichthyosis hystrix, nonepidermolytic palmoplantar keratoderma, annular epidermolytic ichthyosis and keratosis palmoplantaris striata III (PPKS3). PPKS3 is an autosomal dominant disorder affecting palm and sole skin where stratum corneum and epidermal layers are thickened. There is no involvement of nonpalmoplantar skin, and both hair and nails are normal.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Publications for Cytokeratin 17 Antibody (NBP1-32824)(1)

Publication using NBP1-32824

• Kameyama H, Dondapati P, Simmons R, Leslie M et Al. Needle biopsy accelerates pro-metastatic changes and systemic dissemination in breast cancer: Implications for mortality by surgery delay Cell Rep Med 2023-12-20 [PMID: 38118415]

Bioinformatics

• Gene Symbol: KRT17