Cytokeratin 17 Antibody (KRT17/8320R) [DyLight 488]

• Reactivity: Hu
• Applications: IHC
• Clone: KRT17/8320R
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: DyLight 488

Cytokeratin 17 Antibody (KRT17/8320R) [DyLight 488] Summary

• Description: This conjugate is made on demand and is not held in inventory. Each order represents a unique lot. All conjugates are generated using validated conjugation protocols and meet strict degree of labeling (F/P) specifications; additional information is available upon request. The antibody concentration is provided on the individual product label.
  
  For specialized conjugation needs, like large quantities, specific concentrations, and defined F/P ratios, bulk and custom antibody conjugation services are available.
• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: Recombinant fragment of human Cytokeratin 17 protein (exact sequence is proprietary)
• Localization: Cytoplasm.
• Specificity: Antibody to CK17 is an excellent tool to distinguish myoepithelial cells from luminal epithelium of various glands such as mammary, sweat and salivary CK17 is expressed in epithelial cells of various origins, such as bronchial epithelial cells and skin appendages It may be considered as epithelial stem cell marker because CK17 Ab marks basal cell differentiation CK17 is expressed in SCLC much higher than in LADC Eighty-five percent of the triple negative breast carcinomas immunoreact with basal cytokeratins including anti-CK17 Also important is that cases of triple negative breast carcinoma with expression of CK17 show an aggressive clinical course Additionally, anti-CK17 and anti-MUC1 immunoreactivity represents pancreatobiliary subtype whereas anti-MUC2 and anti-CDX-2 positivity defines intestinal subtype
• Isotype: IgG Kappa
• Clonality: Monoclonal
• Host: Rabbit
• Gene: KRT17
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry-Paraffin
• Application Notes: Recommended applications are based on validated applications from the unconjugated base product NBP3-20908. This conjugated antibody is not kept in inventory and is made to order using validated conjugation protocols.

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: 50mM Sodium Borate
• Preservative: 0.05% Sodium Azide
• Purity: Protein A or G purified

Notes

DyLight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.

Alternate Names for Cytokeratin 17 Antibody (KRT17/8320R) [DyLight 488]

• 39.1
• CK-17
• Cytokeratin 17
• cytokeratin-17
• K17
• keratin 17
• keratin 17, type I
• keratin, type I cytoskeletal 17
• keratin-17
• PC
• PC2
• PCHC1

Background

Among the members of the cytokeratin (CK) subfamily of intermediate filament (IF) proteins, CK 17 is normally expressed in the basal cells of complex epithelia but not in stratified or simple epithelia. It has an unusual expression pattern in normal and diseased states and has potential importance in tumor diagnosis. CK 17 synthesis seems to be a marker of basal cell differentiation in complex epithelia and therefore indicative of a certain type of epithelial "stem cells" (1). It has been shown that CK 17 regulates cell growth through binding to the adaptor protein 14-3-3sigma. These findings reveal a new and unexpected role for the intermediate filament cytoskeleton in influencing cell growth and size by regulating protein synthesis (2). CK 17 is expressed in the nail bed, hair follicle, sebaceous glands, and other epidermal appendages (3).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: KRT17