Cytokeratin 17 Antibody (4D2) - Chimeric - BSA Free

• Reactivity: Hu
• Applications: ELISA, IHC
• Clone: 4D2
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

Cytokeratin 17 Antibody (4D2) - Chimeric - BSA Free Summary

• Description: This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG2a format for improved compatibility with existing reagents assays and techniques.
• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: The original antibody was generated by immunizing female BALB/c mice with an immunogenic polypeptide containing amino acids 410-432 (TIVEEVQDGKVISSREQVHQTTR) of human cytokeratin 17 conjugated with KLH. (Uniprot# Q04695)
• Specificity: This antibody is specific for amino acids 410-432 (TIVEEVQDGKVISSREQVHQTTR) of human cytokeratin 17. Cytokeratins are intermediate filaments found in the intracytoplasmic cytoskeleton of epithelial tissue. Cytokeratin 17 regulates the activity of kinases such as PKC and SRC via binding to integrin beta-1 (ITB1) and the receptor of activated protein C kinase 1 (RACK1).
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: KRT17
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunohistochemistry

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Proclin 300
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for Cytokeratin 17 Antibody (4D2) - Chimeric - BSA Free

• 39.1
• CK-17
• Cytokeratin 17
• cytokeratin-17
• K17
• keratin 17
• keratin 17, type I
• keratin, type I cytoskeletal 17
• keratin-17
• PC
• PC2
• PCHC1

Background

Among the members of the cytokeratin (CK) subfamily of intermediate filament (IF) proteins, CK 17 is normally expressed in the basal cells of complex epithelia but not in stratified or simple epithelia. It has an unusual expression pattern in normal and diseased states and has potential importance in tumor diagnosis. CK 17 synthesis seems to be a marker of basal cell differentiation in complex epithelia and therefore indicative of a certain type of epithelial "stem cells" (1). It has been shown that CK 17 regulates cell growth through binding to the adaptor protein 14-3-3sigma. These findings reveal a new and unexpected role for the intermediate filament cytoskeleton in influencing cell growth and size by regulating protein synthesis (2). CK 17 is expressed in the nail bed, hair follicle, sebaceous glands, and other epidermal appendages (3).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: KRT17