Recombinant Human Cytokeratin 16 GST (N-Term) Protein

SDS-Page: Recombinant Human Cytokeratin 16 Protein [H00003868-P01]

Product Discontinued

Recombinant Human Cytokeratin 16 GST (N-Term) Protein Summary

• Description: Recombinant protein with GST tag at N-terminal corresponding to the amino acids 1-473 of Human KRT16
  
  

  Source: Wheat Germ (in vitro)

  Amino Acid Sequence: MTTCSRQFTSSSSMKGSCGIGGGIGGGSSRISSVLAGGSCRAPSTYGGGLSVSSRFSSGGACGLGGGYGGGFSSSSSFGSGFGGGYGGGLGAGFGGGLGAGFGGGFAGGDGLLVGSEKVTMQNLNDRLASYLDKVRALEEANADLEVKIRDWYQRQRPSEIKDYSPYFKTIEDLRNKIIAATIENAQPILQIDNARLAADDFRTKYEHELALRQTVEADVNGLRRVLDELTLARTDLEMQIEGLKEELAYLRKNHEEEMLALRGQTGGDVNVEMDAAPGVDLSRILNEMRDQYEQMAEKNRRDAETWFLSKTEELNKEVASNSELVQSSRSEVTELRRVLQGLEIELQSQLSMKASLENSLEETKGRYCMQLSQIQGLIGSVEEQLAQLRCEMEQQSQEYQILLDVKTRLEQEIATYRRLLEGEDAHLSSQQASGQSYSSREVFTSSSSSSSRQTRPILKEQSSSSFSQGQSS

• Preparation Method: in vitro wheat germ expression system
• Details of Functionality: This protein was produced in an in vitro wheat germ expression system that should preserve correct conformational folding that is necessary for biological function. While it is possible that this protein could display some level of activity, the functionality of this protein has not been explicitly measured or validated.
• Source: Wheat germ
• Protein/Peptide Type: Recombinant Protein
• Gene: KRT16
• Purity: >80% by SDS-PAGE and Coomassie blue staining

Applications/Dilutions

• Dilutions:
  • Dot Blot
  • ELISA
  • Immunoaffinity Purification
  • Protein Array
  • Western Blot
• Application Notes: Use in Dot Blot reported in scientific literature (PMID 27773721).
• Theoretical MW: 77.7 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -80C. Avoid freeze-thaw cycles.
• Buffer: 50 mM Tris-HCl, 10 mM reduced Glutathione, pH 8.0 in the elution buffer.
• Preservative: No Preservative
• Purity: >80% by SDS-PAGE and Coomassie blue staining

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for Recombinant Human Cytokeratin 16 GST (N-Term) Protein

• CK16
• CK-16
• cytokeratin 16
• cytokeratin-16
• FNEPPK
• focal non-epidermolytic palmoplantar keratoderma
• K16
• K1CP
• keratin 16
• keratin, type I cytoskeletal 16
• keratin-16
• KRT16A
• NEPPK

Background

The protein encoded by this gene is a member of the keratin gene family. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. Most of the type I cytokeratins consist of acidic proteins which are arranged in pairs of heterotypic keratin chains and are clustered in a region of chromosome 17q12-q21. This keratin has been coexpressed with keratin 14 in a number of epithelial tissues, including esophagus, tongue, and hair follicles. Mutations in this gene are associated with type 1 pachyonychia congenita, non-epidermolytic palmoplantar keratoderma and unilateral palmoplantar verrucous nevus. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Publications for Cytokeratin 16 Recombinant Protein (H00003868-P01)(1)

Publication using H00003868-P01

• Tajerian M, Hung V, Khan H et al. Identification of KRT16 as a target of an autoantibody response in complex regional pain syndrome. Exp. Neurol. 2016-10-20 [PMID: 27773721] (Cytometric Bead Assay Standard)

Review for Cytokeratin 16 Recombinant Protein (H00003868-P01) (1)

Average Rating: 5

(Based on 1 review)

reviewed by: maral tajerian Binding assay/Protein-protein interaction 01/19/2017

Summary

• Application: Binding assay/Protein-protein interaction
• Comments: I have used this recombinant protein for dot blot analysis (2microliters/dot). Works very well.

Comments

• Comments: Blocking buffer: rockland fluorescent blocking buffer
  Primary antibody: human serum (1:100)
  Secondary antibody: DyLight 800 Goat anti human IgM (1:10k)
  Imaging system: LiCOR odyssey

Bioinformatics

• Gene Symbol: KRT16