Recombinant Human Cytokeratin 16 Protein

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SDS-Page: Recombinant Human Cytokeratin 16 Protein [H00003868-P01]

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, DB, ELISA, PA, AP

Order Details

Recombinant Human Cytokeratin 16 Protein Summary

Description
Recombinant protein with GST-tag at N-terminal corresponding to the amino acids 1 - 473 of Human KRT16 full-length ORF

Source: Wheat Germ (in vitro)

Amino Acid Sequence:MTTCSRQFTSSSSMKGSCGIGGGIGGGSSRISSVLAGGSCRAPSTYGGGLSVSSRFSSGGACGLGGGYGGGFSSSSSFGSGFGGGYGGGLGAGFGGGLGAGFGGGFAGGDGLLVGSEKVTMQNLNDRLASYLDKVRALEEANADLEVKIRDWYQRQRPSEIKDYSPYFKTIEDLRNKIIAATIENAQPILQIDNARLAADDFRTKYEHELALRQTVEADVNGLRRVLDELTLARTDLEMQIEGLKEELAYLRKNHEEEMLALRGQTGGDVNVEMDAAPGVDLSRILNEMRDQYEQMAEKNRRDAETWFLSKTEELNKEVASNSELVQSSRSEVTELRRVLQGLEIELQSQLSMKASLENSLEETKGRYCMQLSQIQGLIGSVEEQLAQLRCEMEQQSQEYQILLDVKTRLEQEIATYRRLLEGEDAHLSSQQASGQSYSSREVFTSSSSSSSRQTRPILKEQSSSSFSQGQSS

Details of Functionality
This protein was produced in an in vitro wheat germ expression system that should preserve correct conformational folding that is necessary for biological function. While it is possible that this protein could display some level of activity, the functionality of this protein has not been explicitly measured or validated.
Source
Wheat germ
Protein/Peptide Type
Recombinant Protein
Gene
KRT16
Purity
>80% by SDS-PAGE and Coomassie blue staining

Applications/Dilutions

Application Notes
Use in Dot Blot reported in scientific literature (PMID 27773721).
Theoretical MW
77.7 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Reviewed Applications
Read 1 Review rated 5
using
H00003868-P01 in the following application:

Publications
Read Publication using H00003868-P01.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
50 mM Tris-HCI, 10 mM reduced Glutathione, pH 8.0.
Purity
>80% by SDS-PAGE and Coomassie blue staining

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for Recombinant Human Cytokeratin 16 Protein

  • CK16
  • CK-16
  • cytokeratin 16
  • cytokeratin-16
  • FNEPPK
  • focal non-epidermolytic palmoplantar keratoderma
  • K16
  • K1CP
  • keratin 16
  • keratin, type I cytoskeletal 16
  • keratin-16
  • KRT16A
  • NEPPK

Background

The protein encoded by this gene is a member of the keratin gene family. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. Most of the type I cytokeratins consist of acidic proteins which are arranged in pairs of heterotypic keratin chains and are clustered in a region of chromosome 17q12-q21. This keratin has been coexpressed with keratin 14 in a number of epithelial tissues, including esophagus, tongue, and hair follicles. Mutations in this gene are associated with type 1 pachyonychia congenita, non-epidermolytic palmoplantar keratoderma and unilateral palmoplantar verrucous nevus. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

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Publications for Cytokeratin 16 Recombinant Protein (H00003868-P01)(1)

We have publications tested in 1 application: DB.


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Review for Cytokeratin 16 Recombinant Protein (H00003868-P01) (1) 51

Average Rating: 5
(Based on 1 review)

Reviews using H00003868-P01:
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 Cytokeratin 16 H00003868-P01
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reviewed by:
maral tajerian
01/19/2017
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Summary

CommentsI have used this recombinant protein for dot blot analysis (2microliters/dot). Works very well.

Comments

CommentsBlocking buffer: rockland fluorescent blocking buffer
Primary antibody: human serum (1:100)
Secondary antibody: DyLight 800 Goat anti human IgM (1:10k)
Imaging system: LiCOR odyssey

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maral tajerian
01/19/2017
Application:
Species:

Bioinformatics

Gene Symbol KRT16