Cytohesin 2 Antibody (CYT2-21) - BSA Free

Western Blot: Cytohesin 2 Antibody (CYT2-21) - BSA Free [NB120-12421] - Whole extract of Rat brain were separated on SDS-PAGE and probed with Monoclonal Anti-Cytohesin 2 antibody produced in Mouse, Clone: CYT2-21. The antibody was developed using 1:1000 Anti-Mouse IgG (Fab specific)-Biotin antibody produced in Goat and 1:10,000 ExtrAvidin-Alkaline.Lane:1. 5 ug/mL antibody2. 10 ug/mL antibody3. Negative Control

• Reactivity: Hu, Rt
• Applications: WB, ELISA, ICC/IF
• Clone: CYT2-21
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 2.0 mg/ml

Cytohesin 2 Antibody (CYT2-21) - BSA Free Summary

• Description: Novus Biologicals Mouse Cytohesin 2 Antibody (CYT2-21) - BSA Free (NB120-12421) is a monoclonal antibody validated for use in WB, ELISA and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant full length protein (Human).
• Localization: Plasma membrane
• Specificity: does not cross-react with other members of the cytohesin family.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: CYTH2
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Immunocytochemistry/ Immunofluorescence 1:10-1:2000
  • Western Blot 5 - 10 ug/ml
• Application Notes: ELISA: Use at an assay dependent dilution. ICC: Use at an assay dependent dilution. Western Blot: Use at a concentration of 5 - 10 ug/ml, this concentration is determined using a whole extract of rat brain. Predicted molecular weight: 40 kDa. Optimal dilutions/concentrations should be determined by the end user.
• Theoretical MW: 40 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control:

  Cytohesin 2 Overexpression Lysate

Reactivity Notes

Cross-reacts with Human and Rat.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 10mM PBS (pH 7.4)
• Preservative: 0.09% Sodium Azide
• Concentration: 2.0 mg/ml
• Purity: Protein A or G purified

Alternate Names for Cytohesin 2 Antibody (CYT2-21) - BSA Free

• ARF exchange factor
• ARF nucleotide-binding site opener
• ARNO
• ARNOPH, SEC7 and coiled-coil domain-containing protein 2
• CTS18.1
• CYTH2
• Cytohesin 2
• pleckstrin homology, Sec7 and coiled-coil domains 2 (cytohesin-2)
• Protein ARNO
• PSCD2
• PSCD2L
• PSCD2LCTS18
• PSCD2pleckstrin homology, Sec7 and coiled-coil domains 2
• Sec7 and coiled/coil domains 2 (cytohesin-2)

Background

The ADP-ribosylation factor (Arf) family comprises a group of structurally and functionally conserved 21 kDa proteins, which are members of the Ras superfamily of regulatory GTP-binding proteins.
Arf is involved in intracellular protein traffic to and within the Golgi complex. Arf has a number of disparate activities including maintenance of organelle integrity, assembly of coat proteins, as a co-factor for cholera toxin and as an activator of phospholipase D.
Like other small GTPases, Arf is found to be active when bound to GTP and inactive when bound to GDP. Arf's activation is dependent upon guanine nucleotide exchange factors (GEFs) which increase the rate of exchange of bound GDP with GTP. All GEFs have a highly conserved Sec7 domain. GEF activity lies in the Sec7 domain and this activity has been shown to be inhibited by the fungal metabolite brefeldin-A (BFA). A small group of GEFs which are insensitive to brefeldin-A (BFA) include cytohesin-1 (B2-1), cytohesin-2 (ARNO), cytohesin-3 (ARNO3), and cytohesin-4. All cytohesins function in the cell periphery and contain a pleckstrin homology (PH) domain. The PH domain has been shown to interact with phosphatidylinositol 3,4,5-triphosphate and is believed to promote membrane targeting of the cytohesins. Recruitment of the cytohesins to the membranes can occur in response to tyrosine kinase receptor activation. This response appears to require the activation of phosphatidylinositol 3-kinase (PI 3-kinase).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CYTH2
• Entrez:
  • 9266(Human)
  • 116692(Rat)