Cytoglobin Antibody

Images

 
Western Blot: Cytoglobin Antibody [NBP2-16084] - Whole cell extract (30 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with Cytoglobin antibody diluted at 1:1000.
Immunocytochemistry/ Immunofluorescence: Cytoglobin Antibody [NBP2-16084] - Immunofluorescence analysis of methanol-fixed A431, using antibody at 1:500 dilution.
Western Blot: Cytoglobin Antibody [NBP2-16084] - Sample (30 ug of whole cell lysate) A: IMR32 12% SDS PAGE gel, diluted at 1:2000.
Western Blot: Cytoglobin Antibody [NBP2-16084] - Sample (50 ug of whole cell lysate) A: Mouse Heart, 12% SDS PAGE gel, diluted at 1:1000.

Product Details

Summary
Reactivity Hu, Mu, Rt, Bv, RM, ShSpecies Glossary
Applications WB, ICC/IF, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

Cytoglobin Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human Cytoglobin. The exact sequence is proprietary.
Localization
Cytoplasm
Predicted Species
Bovine (95%), Rhesus Macaque (97%), Sheep (96%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
CYGB
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:3000
  • Immunocytochemistry/Immunofluorescence 1:100-1:1000
  • Immunohistochemistry-Paraffin
Application Notes
IHC-P usage reported in scientific literature (PMID: 26835715).
Theoretical MW
21 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publication using
NBP2-16084 in the following applications:

Reactivity Notes

Dog (82%).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.0), 1.0% BSA and 20% Glycerol
Preservative
0.01% Thimerosal
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Cytoglobin Antibody

  • cytoglobin
  • HGBHistoglobin
  • histoglobin
  • STAPHGb
  • Stellate cell activation-associated protein

Background

Cytoglobin is a ubiquitously expressed hexacoordinate hemoglobin that may facilitate diffusion of oxygen through tissues, scavenge nitric oxide or other reactive oxygen species, or serve a protective function during oxidative stress (Trent and Hargrove, 2002 [PubMed 11893755]).[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Cytoglobin Antibody (NBP2-16084)(1)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 1 application: IHC-P.


Filter By Application
IHC-P
(1)
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Filter By Species
Mouse
(1)
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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol CYGB
Uniprot