Cytoglobin Antibody

Western Blot: Cytoglobin Antibody [NBP2-16084] - Whole cell extract (30 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with Cytoglobin antibody diluted at 1:1000.

Immunocytochemistry/Immunofluorescence: Cytoglobin Antibody [NBP2-16084] - Immunofluorescence analysis of methanol-fixed A431, using antibody at 1:500 dilution.

Western Blot: Cytoglobin Antibody [NBP2-16084] - Sample (30 ug of whole cell lysate) A: IMR32 12% SDS PAGE gel, diluted at 1:2000.

Western Blot: Cytoglobin Antibody [NBP2-16084] - Sample (50 ug of whole cell lysate) A: Mouse Heart, 12% SDS PAGE gel, diluted at 1:1000.

• Reactivity: Hu, Mu, Rt, Bv, RM, Sh
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

Cytoglobin Antibody Summary

• Immunogen: Recombinant protein encompassing a sequence within the center region of human Cytoglobin. The exact sequence is proprietary.
• Localization: Cytoplasm
• Predicted Species: Bovine (95%), Rhesus Macaque (97%), Sheep (96%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: CYGB
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin Reported in scientific literature (PMID: 26835715)
  • Western Blot 1:500-1:3000
• Theoretical MW: 21 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Dog (82%).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS, 1% BSA, 20% Glycerol
• Preservative: 0.01% Thimerosal
• Purity: Antigen Affinity-purified

Alternate Names for Cytoglobin Antibody

• cytoglobin
• HGBHistoglobin
• histoglobin
• STAPHGb
• Stellate cell activation-associated protein

Background

Cytoglobin is a ubiquitously expressed hexacoordinate hemoglobin that may facilitate diffusion of oxygen through tissues, scavenge nitric oxide or other reactive oxygen species, or serve a protective function during oxidative stress (Trent and Hargrove, 2002 [PubMed 11893755]).[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Publications for Cytoglobin Antibody (NBP2-16084)(1)

Publication using NBP2-16084

• Svenson S, Case RI, Cole RO et al. Tumor Selective Silencing Using an RNAi-Conjugated Polymeric Nanopharmaceutical. Mol. Pharm. 2016-03-07 [PMID: 26835715] (IHC-P, Mouse)

Bioinformatics

• Gene Symbol: CYGB
• Uniprot:
  • Q8WWM9(Human)