Cytochrome P450 3A5 Overexpression Lysate (Native)

Images

 
Western Blot: Cytochrome P450 3A5 Overexpression Lysate (Adult Normal) [NBL1-09693] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Cytochrome P450 3A5.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

Cytochrome P450 3A5 Overexpression Lysate (Native) Summary

Immunogen
The lysate was created in HEK293T cells, using plasmid ID RC207432 and based on accession number NM_000777. The protein contains a C-terminal DDK tag.
Specificity
Homo sapiens cytochrome P450, family 3, subfamily A, polypeptide 5 (CYP3A5), mRNA.
Gene
CYP3A5

Applications/Dilutions

Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Theoretical MW
56.9 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for Cytochrome P450 3A5

Type
Overexpression
Protein State
Native

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for Cytochrome P450 3A5 Overexpression Lysate (Native)

  • aryl hydrocarbon hydroxylase
  • CP35
  • CYPIIIA5
  • cytochrome P450 3A5
  • Cytochrome P450 HLp2
  • cytochrome P-450
  • cytochrome P450, family 3, subfamily A, polypeptide 5
  • cytochrome P450, subfamily IIIA (niphedipine oxidase), polypeptide 5
  • Cytochrome P450-PCN3
  • DKFZp686L16231
  • EC 1.14.14.1
  • flavoprotein-linked monooxygenase
  • microsomal monooxygenase
  • niphedipine oxidase
  • P450PCN3FLJ31317
  • PCN3
  • xenobiotic monooxygenase

Background

This gene,CYP3A5, encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the endoplasmic reticulum and its expression is induced by glucocorticoids and some pharmacological agents. The enzyme metabolizes drugs such as nifedipine and cyclosporine as well as the steroid hormones testosterone, progesterone and androstenedione. This gene is part of a cluster of cytochrome P450 genes on chromosome 7q21.1. This cluster includes a pseudogene, CYP3A5P1, which is very similar to CYP3A5. This similarity has caused some difficulty in determining whether cloned sequences represent the gene or the pseudogene. [provided by RefSeq]. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Publications for Cytochrome P450 3A5 Lysate (NBL1-09693) (0)

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Bioinformatics

Gene Symbol CYP3A5