![Western Blot: Cytochrome P450 3A5 Antibody (F18P3B6) [NBP2-50209] - Western Blot of recombinant CYP450s](http://images.novusbio.com/fullsize2/Cytochrome-P450-3A5-Antibody-(F18P3B6)-Western-Blot-NBP2-50209-img0001.jpg "Western Blot: Cytochrome P450 3A5 Antibody (F18P3B6) [NBP2-50209] - Western Blot of recombinant CYP450s")
| Reactivity | HuSpecies Glossary |
| Applications | WB, ELISA, IHC |
| Clone | F18P3B6 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Format | BSA Free |
| Concentration | 1 mg/ml |
| Immunogen | Ovalbumin-conjugated synthetic peptide DSRDGTLSGE (C-terminal sequence) |
| Isotype | IgG1 Kappa |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | CYP3A5 |
| Purity | Protein G purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | Antigen retrieval: microwave 20 min @ 800W in 10 mM citrate buffer, pH 6.0 |
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| Reviewed Applications |
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| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS |
| Preservative | 0.02% Sodium Azide |
| Concentration | 1 mg/ml |
| Purity | Protein G purified |
![ELISA: Cytochrome P450 3A4 Antibody (3H8) [NBP2-37502] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);](http://images.novusbio.com/images2/Cytochrome-P450-3A4-Antibody-(3H8)-ELISA-NBP2-37502-img0001.jpg)
![Western Blot: Cytochrome P450 3A4 Antibody (3H8) [NBP2-37502] - Analysis using CYP3A4 mAb against human CYP3A4 (AA: 243-430) recombinant protein. (Expected MW is 47.5 kDa)](http://images.novusbio.com/images2/Cytochrome-P450-3A4-Antibody-(3H8)-Western-Blot-NBP2-37502-img0002.jpg)
![Western Blot: Cytochrome P450 3A4 Antibody (3H8) [NBP2-37502] - Analysis using CYP3A4 mAb against HEK293 (1) and CYP3A4 (AA: 243-430)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/images2/Cytochrome-P450-3A4-Antibody-(3H8)-Western-Blot-NBP2-37502-img0003.jpg)
![SDS-Page: Recombinant Human AHRR Protein [H00057491-Q01] - 12.5% SDS-PAGE Stained with Coomassie Blue.](http://images.novusbio.com/images/qc_test-H00057491-Q01-1.jpg)
![Immunohistochemistry-Paraffin: MDR1/ABCB1 Antibody (SN06-42) [NBP2-67667] - Analysis of paraffin-embedded human liver tissue using anti-P Glycoprotein antibody. Counter stained with hematoxylin.](http://images.novusbio.com/images2/MDR1-ABCB1-Antibody-(SN06-42)-Immunohistochemistry-Paraffin-NBP2-67667-img0001.jpg)
![Immunohistochemistry-Frozen: MDR1/ABCB1 Antibody (SN06-42) [NBP2-67667] - Mouse (B6) liver tissue. Fixed in ice-cold methanol for 10 minutes. Blocking in 10% serum and 1% BSA. Primary antibody at 5 ug/mL, overnight incubation. Secondary antibody at 2 ug/mL for 1.5 hour (Invitrogen A-11012). Mounting with Vector Vectashield with DAPI. Image from a verified customer review.](http://images.novusbio.com/images2/MDR1-ABCB1-Antibody-(SN06-42)-Immunohistochemistry-Frozen-NBP2-67667-img0004.jpg)
![Immunohistochemistry-Paraffin: MDR1/ABCB1 Antibody (SN06-42) [NBP2-67667] - Analysis of paraffin-embedded mouse kidney tissue using anti-P Glycoprotein antibody. Counter stained with hematoxylin.](http://images.novusbio.com/images2/MDR1-ABCB1-Antibody-(SN06-42)-Immunohistochemistry-Paraffin-NBP2-67667-img0003.jpg)
![Western Blot: Cytochrome P450 2C19 Antibody [NBP3-10546] - Western blot analysis using NBP3-10546 on Human Placenta as a positive control. Antibody Titration: 0.2-1 ug/ml](http://images.novusbio.com/images2/Cytochrome-P450-2C19-Antibody-Western-Blot-NBP3-10546-img0001.jpg)
![Flow Cytometry: Cytochrome P450 2C9 Antibody (1D7) [NBP2-01397] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-Cytochrome P450 2C9 antibody, and then analyzed by flow cytometry.](http://images.novusbio.com/images/Cytochrome-P450-2C9-Antibody-(1D7)-Flow-Cytometry-NBP2-01397-img0001.jpg)
![Western Blot: Cytochrome P450 2C9 Antibody (1D7) [NBP2-01397] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Cytochrome P450 2C9 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Cytochrome P450 2C9.](http://images.novusbio.com/images/Cytochrome-P450-2C9-Antibody-(1D7)-Western-Blot-NBP2-01397-img0002.jpg)
![Immunohistochemistry-Paraffin: Cytochrome P450 2C9 Antibody (1D7) [NBP2-01397] - Staining of paraffin-embedded Carcinoma of Human liver tissue using anti-Cytochrome P450 2C9 mouse monoclonal antibody.](http://images.novusbio.com/images/Cytochrome-P450-2C9-Antibody-(1D7)-Immunohistochemistry-Paraffin-NBP2-01397-img0003.jpg)
![Immunohistochemistry-Paraffin: Cytochrome P450 1A2 Antibody (OTI8F1) [NBP2-02563] - Staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-Cytochrome P450 1A2 mouse monoclonal antibody.](http://images.novusbio.com/images2/Cytochrome-P450-1A2-Antibody-(OTI8F1)-Immunohistochemistry-Paraffin-NBP2-02563-img0006.jpg)
![Immunohistochemistry-Paraffin: Cytochrome P450 1A2 Antibody (OTI8F1) [NBP2-02563] - Staining of paraffin-embedded Human colon tissue using anti-Cytochrome P450 1A2 mouse monoclonal antibody.](http://images.novusbio.com/images2/Cytochrome-P450-1A2-Antibody-(OTI8F1)-Immunohistochemistry-Paraffin-NBP2-02563-img0007.jpg)
![Immunohistochemistry-Paraffin: Cytochrome P450 1A2 Antibody (OTI8F1) [NBP2-02563] - Staining of paraffin-embedded Human Kidney tissue using anti-Cytochrome P450 1A2 mouse monoclonal antibody.](http://images.novusbio.com/images2/Cytochrome-P450-1A2-Antibody-(OTI8F1)-Immunohistochemistry-Paraffin-NBP2-02563-img0008.jpg)
![SDS-Page: Recombinant Human CYP3A7 Protein [H00001551-P01] - 12.5% SDS-PAGE Stained with Coomassie Blue.](http://images.novusbio.com/images/qc_test-H00001551-P01-1.jpg)
![Immunohistochemistry-Paraffin: TBC1D9 Antibody [NBP2-13415] - Staining of human testis shows moderate cytoplasmic positivity in cells in seminiferous ducts.](http://images.novusbio.com/images2/TBC1D9-Antibody-Immunohistochemistry-Paraffin-NBP2-13415-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: TBC1D9 Antibody [NBP2-13415] - Staining of human cell line U-2 OS shows localization to nucleoplasm & cytosol.](http://images.novusbio.com/images2/TBC1D9-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-13415-img0003.jpg)
![Western Blot: Cytochrome P450 2A6 Antibody (OTI1D2) [NBP2-01437] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Cytochrome P450 2A6 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Cytochrome P450 2A6.](http://images.novusbio.com/images2/Cytochrome-P450-2A6-Antibody-(OTI1D2)-Western-Blot-NBP2-01437-img0001.jpg)
![Immunohistochemistry-Paraffin: Cytochrome P450 2A6 Antibody (OTI1D2) [NBP2-01437] - Staining of paraffin-embedded Human bladder tissue using anti-Cytochrome P450 2A6 mouse monoclonal antibody.](http://images.novusbio.com/images2/Cytochrome-P450-2A6-Antibody-(OTI1D2)-Immunohistochemistry-Paraffin-NBP2-01437-img0005.jpg)
![Western Blot: Cytochrome P450 2A6 Antibody (OTI1D2) [NBP2-01437] - Analysis of extracts (35ug) from 9 different cell lines by using anti-Cytochrome P450 2A6 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).](http://images.novusbio.com/images2/Cytochrome-P450-2A6-Antibody-(OTI1D2)-Western-Blot-NBP2-01437-img0009.jpg)
![Western Blot: Cytochrome P450 2C8 Antibody [NBP1-88054] - Analysis using Anti-CYP2C8 antibody NBP1-88054 (A) shows similar pattern to independent antibody NBP1-88055 (B).](http://images.novusbio.com/images2/Cytochrome-P450-2C8-Antibody-Western-Blot-NBP1-88054-img0009.jpg)
![Immunohistochemistry-Paraffin: Cytochrome P450 2C8 Antibody [NBP1-88054] - Analysis in human liver and skin tissues. Corresponding CYP2C8 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/images2/Cytochrome-P450-2C8-Antibody-Immunohistochemistry-Paraffin-NBP1-88054-img0013.jpg)
![Immunohistochemistry-Paraffin: Cytochrome P450 2C8 Antibody [NBP1-88054] - Staining of human cerebellum shows no positivity in Purkinje cells as expected.](http://images.novusbio.com/images2/Cytochrome-P450-2C8-Antibody-Immunohistochemistry-Paraffin-NBP1-88054-img0014.jpg)
| Images | Ratings | Applications | Species | Date | Details | ||||||||||
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Enlarge |
reviewed by:
Dahao Ling |
Simple Western | Human and Mouse | 11/07/2019 |
Summary
Comments
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Secondary Antibodies |
Isotype Controls |
Diseases for Cytochrome P450 3A5 Antibody (NBP2-50209)Discover more about diseases related to Cytochrome P450 3A5 Antibody (NBP2-50209).
| Pathways for Cytochrome P450 3A5 Antibody (NBP2-50209)View related products by pathway.
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| Dahao Ling 11/07/2019 |
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| Application: | Simple Western | |
| Species: | Human and Mouse |
| Gene Symbol | CYP3A5 |
![Immunohistochemistry: Cytochrome P450 3A5 Antibody (F18P3B6) [NBP2-50209] - Staining of CYP3A5 in an oesophagus cancer cross section.](http://images.novusbio.com/fullsize2/Cytochrome-P450-3A5-Antibody-(F18P3B6)-Immunohistochemistry-NBP2-50209-img0002.jpg "Immunohistochemistry: Cytochrome P450 3A5 Antibody (F18P3B6) [NBP2-50209] - Staining of CYP3A5 in an oesophagus cancer cross section.")
![Immunocytochemistry/Immunofluorescence: Cytochrome P450 1A1/1A2 Antibody (MC1) [NB100-74398] - Analysis of Cytochrome P450 1A1/1A2 (green) showing staining in the cytoplasm and membrane of HT29 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a Cytochrome P450 1A1/1A2 monoclonal antibody in 3% BSA-PBS at a dilution of 1:100 and incubated overnight at 4C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a fluorescentred phalloidin and nuclei (blue) were stained with Hoechst or DAPI.](http://images.novusbio.com/images2/Cytochrome-P450-1A1-1A2-Antibody-(MC1)-Immunocytochemistry-Immunofluorescence-NB100-74398-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: Cytochrome P450 1A1/1A2 Antibody (MC1) [NB100-74398] - Analysis of Cytochrome P450 1A1/1A2 (green) showing staining in the cytoplasm and membrane of HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a Cytochrome P450 1A1/1A2 monoclonal antibody in 3% BSA-PBS at a dilution of 1:100 and incubated overnight at 4C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a fluorescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI.](http://images.novusbio.com/images2/Cytochrome-P450-1A1-1A2-Antibody-(MC1)-Immunocytochemistry-Immunofluorescence-NB100-74398-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: Cytochrome P450 1A1/1A2 Antibody (MC1) [NB100-74398] - Analysis of Cytochrome P450 1A1/1A2 (green) showing staining in the cytoplasm and membrane of COS-7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a Cytochrome P450 1A1/1A2 monoclonal antibody in 3% BSA-PBS at a dilution of 1:100 and incubated overnight at 4C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a fluorescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI.](http://images.novusbio.com/images2/Cytochrome-P450-1A1-1A2-Antibody-(MC1)-Immunocytochemistry-Immunofluorescence-NB100-74398-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: Cytochrome P450 2D6 Antibody (JM44-23) [NBP2-67020] - Staining Cytochrome P450 2D6 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/images2/Cytochrome-P450-2D6-Antibody-(JM44-23)-Immunocytochemistry-Immunofluorescence-NBP2-67020-img0001.jpg)
![Immunohistochemistry-Paraffin: Cytochrome P450 2D6 Antibody (JM44-23) [NBP2-67020] - Analysis of paraffin-embedded human kidney tissue using anti-Cytochrome P450 2D6 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/images2/Cytochrome-P450-2D6-Antibody-(JM44-23)-Immunohistochemistry-Paraffin-NBP2-67020-img0002.jpg)
![Immunohistochemistry-Paraffin: Cytochrome P450 2D6 Antibody (JM44-23) [NBP2-67020] - Analysis of paraffin-embedded human liver tissue using anti-Cytochrome P450 2D6 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/images2/Cytochrome-P450-2D6-Antibody-(JM44-23)-Immunohistochemistry-Paraffin-NBP2-67020-img0003.jpg)
![Flow Cytometry: Cytochrome P450 2B6 Antibody (3D5) [NBP2-01800] - Analysis of Jurkat cells, using anti-Cytochrome P450 2B6 antibody, (Red), compared to a nonspecific negative control antibody (Blue).](http://images.novusbio.com/images2/Cytochrome-P450-2B6-Antibody-(3D5)-Flow-Cytometry-NBP2-01800-img0013.jpg)
![Flow Cytometry: Cytochrome P450 2B6 Antibody (3D5) [NBP2-01800] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-Cytochrome P450 2B6 antibody, and then analyzed by flow cytometry.](http://images.novusbio.com/images2/Cytochrome-P450-2B6-Antibody-(3D5)-Flow-Cytometry-NBP2-01800-img0014.jpg)
![Immunocytochemistry/Immunofluorescence: Cytochrome P450 2B6 Antibody (3D5) [NBP2-01800] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY Cytochrome P450 2B6.](http://images.novusbio.com/images2/Cytochrome-P450-2B6-Antibody-(3D5)-Immunocytochemistry-Immunofluorescence-NBP2-01800-img0015.jpg)
![Immunocytochemistry/Immunofluorescence: AHR Antibody [NB100-128] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton X-100. The cells were incubated with anti AHR [NB100-128] at a 1:100 dilution overnight at 4C and detected with an anti-goat DyLight 488 (Green) at a 1:500 dilution. Actin was detected with Phalloidin 568 (Red) at a 1:200 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/images2/AHR-Antibody-Immunocytochemistry-Immunofluorescence-NB100-128-img0004.jpg)
![Gel Super Shift Assays: AHR Antibody [NB100-128] - EMSA validation of AHR antibody on U937 cells nuclear protein. Image submitted by verified customer - see review for protocol details.](http://images.novusbio.com/images2/AHR-Antibody-Gel-Super-Shift-Assays-NB100-128-img0003.jpg)
![Immunohistochemistry-Paraffin: AHR Antibody [NB100-128] - Analysis of FFPE human liver using 1:200 conc. of AHR antibody on a Bond Rx autostainer (Leica Biosystems). The assay involved 20 minutes of heat induced antigen retrieval (HIER) using 10mM sodium citrate buffer (pH 6.0) and endogenous peroxidase quenching with peroxide block. The sections were incubated with primary antibody for 30 minutes and Bond Polymer Refine Detection (Leica Biosystems) with DAB was used for signal development followed by counterstaining with hematoxylin. Whole slide scanning and capturing of representative images was performed using Aperio AT2 (Leica Biosystems). Staining was performed by Histowiz.](http://images.novusbio.com/images2/AHR-Antibody-Immunohistochemistry-Paraffin-NB100-128-img0006.jpg)
![Western Blot: Cytochrome P450 2E1 Antibody [NBP1-85367] - Western blot analysis in mouse liver tissue and rat liver tissue.](http://images.novusbio.com/images2/Cytochrome-P450-2E1-Antibody-Western-Blot-NBP1-85367-img0011.jpg)
![Immunohistochemistry-Paraffin: Cytochrome P450 2E1 Antibody [NBP1-85367] - Staining of human endometrium, liver, liver cancer and tonsil using Anti-CYP2E1 antibody NBP1-85367 (A) shows similar protein distribution across tissues to independent antibody NBP1-85366 (B).](http://images.novusbio.com/images2/Cytochrome-P450-2E1-Antibody-Immunohistochemistry-Paraffin-NBP1-85367-img0018.jpg)
![Immunohistochemistry-Paraffin: Cytochrome P450 2E1 Antibody [NBP1-85367] - Analysis in human liver and endometrium tissues. Corresponding CYP2E1 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/images2/Cytochrome-P450-2E1-Antibody-Immunohistochemistry-Paraffin-NBP1-85367-img0019.jpg)
![PXR/NR1I2 Antibody (H4417) [Unconjugated]](/sites/all/modules/enterprise-tech/et_datasheets/images/novus_guarantee.png "PXR/NR1I2 Antibody (H4417) [Unconjugated]")
![Sandwich ELISA: Histatin 3 Antibody (4G9) [H00003347-M01] - Detection limit for recombinant GST tagged HTN3 is approximately 3ng/ml as a capture antibody.](http://images.novusbio.com/images2/Histatin-3-Antibody-(4G9)-Sandwich-ELISA-H00003347-M01-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: Histatin 3 Antibody (4G9) [H00003347-M01] - Analysis of monoclonal antibody to HTN3 on HeLa cell . Antibody concentration 10 ug/ml.](http://images.novusbio.com/images2/Histatin-3-Antibody-(4G9)-Immunocytochemistry-Immunofluorescence-H00003347-M01-img0005.jpg)
![Flow Cytometry: Mouse IgG1 Kappa Isotype Control (P3.6.2.8.1) [NBP1-43319] - Analysis of Alexa Fluor (R) 700 conjugate of NBP1-43319. Human buffy coat stained with mouse IgG1 Kappa isotype control. Flow cytometry image submitted by a verified customer review.](http://images.novusbio.com/images2/Mouse-IgG1-Kappa-Light-Chain-Isotype-Control-(P3.6.2.8.1)-Flow-Cytometry-NBP1-43319-img0002.jpg "Flow Cytometry: Mouse IgG1 Kappa Isotype Control (P3.6.2.8.1) [NBP1-43319] - Analysis of Alexa Fluor (R) 700 conjugate of NBP1-43319. Human buffy coat stained with mouse IgG1 Kappa isotype control. Flow cytometry image submitted by a verified customer review.")
