![Western Blot: Cytochrome P450 3A5 Antibody (F18P3B6) [NBP2-50209] - Western Blot of recombinant CYP450s](http://images.novusbio.com/fullsize2/Cytochrome-P450-3A5-Antibody-(F18P3B6)-Western-Blot-NBP2-50209-img0001.jpg "Western Blot: Cytochrome P450 3A5 Antibody (F18P3B6) [NBP2-50209] - Western Blot of recombinant CYP450s")
| Reactivity | HuSpecies Glossary |
| Applications | WB, ELISA, IHC |
| Clone | F18P3B6 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Concentration | 1 mg/ml |
| Immunogen | Ovalbumin-conjugated synthetic peptide DSRDGTLSGE (C-terminal sequence) |
| Isotype | IgG1 Kappa |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | CYP3A5 |
| Purity | Protein G purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | Antigen retrieval: microwave 20 min @ 800W in 10 mM citrate buffer, pH 6.0 |
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| Reviewed Applications |
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| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS |
| Preservative | 0.02% Sodium Azide |
| Concentration | 1 mg/ml |
| Purity | Protein G purified |
| Images | Ratings | Applications | Species | Date | Details | ||||||||||
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reviewed by:
Dahao Ling |
Simple Western | Mouse and Human | 11/07/2019 |
Summary
Comments
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Secondary Antibodies |
Isotype Controls |
| Alexa Fluor 350 | NBP2-50209AF350 | |
| Alexa Fluor 405 | NBP2-50209AF405 | |
| Alexa Fluor 488 | NBP2-50209AF488 | |
| Alexa Fluor 532 | NBP2-50209AF532 | |
| Alexa Fluor 594 | NBP2-50209AF594 | |
| Alexa Fluor 647 | NBP2-50209AF647 | |
| Alexa Fluor 700 | NBP2-50209AF700 | |
| Alexa Fluor 750 | NBP2-50209AF750 | |
| Biotin | NBP2-50209B | |
| DyLight 350 | NBP2-50209UV | |
| DyLight 405 | NBP2-50209V | |
| DyLight 488 | NBP2-50209G | |
| DyLight 550 | NBP2-50209R | |
| DyLight 594 | NBP2-50209DL594 | |
| DyLight 650 | NBP2-50209C | |
| DyLight 680 | NBP2-50209FR | |
| DyLight 755 | NBP2-50209IR | |
| FITC | NBP2-50209F | |
| HRP | NBP2-50209H | |
| Janelia Fluor 549 | NBP2-50209JF549 | |
| Janelia Fluor 646 | NBP2-50209JF646 |
Diseases for Cytochrome P450 3A5 Antibody (NBP2-50209)Discover more about diseases related to Cytochrome P450 3A5 Antibody (NBP2-50209).
| Pathways for Cytochrome P450 3A5 Antibody (NBP2-50209)View related products by pathway.
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| Dahao Ling 11/07/2019 |
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| Application: | Simple Western | |
| Species: | Mouse and Human |
| Gene Symbol | CYP3A5 |
![Immunohistochemistry: Cytochrome P450 3A5 Antibody (F18P3B6) [NBP2-50209] - Staining of CYP3A5 in an oesophagus cancer cross section.](http://images.novusbio.com/fullsize2/Cytochrome P450 3A5 Antibody (F18P3B6)-Immunohistochemistry-NBP2-50209-img0002.jpg "Immunohistochemistry: Cytochrome P450 3A5 Antibody (F18P3B6) [NBP2-50209] - Staining of CYP3A5 in an oesophagus cancer cross section.")
![Western Blot: Cytochrome P450 3A4 Antibody (3H8) [NBP2-37502] - Analysis using CYP3A4 mAb against HEK293 (1) and CYP3A4 (AA: 243-430)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/images2/Cytochrome-P450-3A4-Antibody-(3H8)-Western-Blot-NBP2-37502-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: Cytochrome P450 3A4 Antibody (3H8) [NBP2-37502] - Analysis of HepG2 cells using CYP3A4 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.](http://images.novusbio.com/images2/Cytochrome-P450-3A4-Antibody-(3H8)-Immunocytochemistry-Immunofluorescence-NBP2-37502-img0004.jpg)
![Immunohistochemistry-Paraffin: Cytochrome P450 3A4 Antibody (3H8) [NBP2-37502] - Analysis of human brain tissues using CYP3A4 mouse mAb with DAB staining.](http://images.novusbio.com/images2/Cytochrome-P450-3A4-Antibody-(3H8)-Immunohistochemistry-NBP2-37502-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: Cytochrome P450 1A1/1A2 Antibody (MC1) [NB100-74398] - Analysis of Cytochrome P450 1A1/1A2 (green) showing staining in the cytoplasm and membrane of COS-7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a Cytochrome P450 1A1/1A2 monoclonal antibody in 3% BSA-PBS at a dilution of 1:100 and incubated overnight at 4C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a fluorescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI.](http://images.novusbio.com/images2/Cytochrome-P450-1A1-1A2-Antibody-(MC1)-Immunocytochemistry-Immunofluorescence-NB100-74398-img0003.jpg)
![Immunohistochemistry-Paraffin: Cytochrome P450 1A1/1A2 Antibody (MC1) [NB100-74398] - Analysis showing positive staining in the cytoplasm and membrane of Mouse liver tissue (right) compared with a negative control in the absence of primary antibody (left).](http://images.novusbio.com/images2/Cytochrome-P450-1A1-1A2-Antibody-(MC1)-Immunohistochemistry-Paraffin-NB100-74398-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: Cytochrome P450 1A1/1A2 Antibody (MC1) [NB100-74398] - Analysis of Cytochrome P450 1A1/1A2 (green) showing staining in the cytoplasm and membrane of HT29 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a Cytochrome P450 1A1/1A2 monoclonal antibody in 3% BSA-PBS at a dilution of 1:100 and incubated overnight at 4C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a fluorescentred phalloidin and nuclei (blue) were stained with Hoechst or DAPI.](http://images.novusbio.com/images2/Cytochrome-P450-1A1-1A2-Antibody-(MC1)-Immunocytochemistry-Immunofluorescence-NB100-74398-img0001.jpg)
![SDS-Page: Recombinant Human AHRR Protein [H00057491-Q01] - 12.5% SDS-PAGE Stained with Coomassie Blue.](http://images.novusbio.com/images/qc_test-H00057491-Q01-1.jpg)
![Western Blot: MDR1/ABCB1 Antibody [NB100-80870] - Analyis of anti-ABCB1 antibody shows detection of ABCB1 in crude membrane extracts from HF insect cells over-expressing human ABCB1. The extract was loaded onto a gel in the amounts indicated followed by electrophoresis and transfer to nitrocellulose. The membrane was probed with the primary antibody diluted to 1:600, followed by Peroxidase Conjugated Affinity Purified Anti-RABBIT IgG at 1:10000. Personal Communication, Anna Calcagno, CCR-NCI, Bethesda, MD.](http://images.novusbio.com/images2/MDR1-ABCB1-Antibody-Western-Blot-NB100-80870-img0002.jpg)
![Immunohistochemistry-Paraffin: MDR1/ABCB1 Antibody [NB100-80870] - Staining of human kidney tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.](http://images.novusbio.com/images2/MDR1-ABCB1-Antibody-Immunohistochemistry-Paraffin-NB100-80870-img0003.jpg)
![Western Blot: Cytochrome P450 2C9 Antibody (1D7) [NBP2-01397] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Cytochrome P450 2C9 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Cytochrome P450 2C9.](http://images.novusbio.com/images/Cytochrome P450 2C9 Antibody (1D7)-Western Blot-NBP2-01397-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: Cytochrome P450 2C9 Antibody (1D7) [NBP2-01397] Staining of COS7 cells transiently transfected by pCMV6-ENTRY Cytochrome P450 2C9.](http://images.novusbio.com/images/Cytochrome P450 2C9 Antibody (1D7)-Immunocytochemistry-Immunofluorescence-NBP2-01397-img0005.jpg)
![Immunohistochemistry-Paraffin: Cytochrome P450 2C9 Antibody (1D7) [NBP2-01397] - Staining of paraffin-embedded Human liver tissue using anti-Cytochrome P450 2C9 mouse monoclonal antibody.](http://images.novusbio.com/images/Cytochrome P450 2C9 Antibody (1D7)-Immunohistochemistry-Paraffin-NBP2-01397-img0004.jpg)
![Western Blot: Cytochrome P450 2D6 Antibody (JM44-23) [NBP2-67020] - Analysis of Cytochrome P450 2D6 on HepG2 cell using anti-Cytochrome P450 2D6 antibody at 1/1,000 dilution.](http://images.novusbio.com/images2/Cytochrome-P450-2D6-Antibody-(JM44-23)-Western-Blot-NBP2-67020-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: Cytochrome P450 2D6 Antibody (JM44-23) [NBP2-67020] - Staining Cytochrome P450 2D6 in SW480 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/images2/Cytochrome P450 2D6 Antibody (JM44-23)-Immunocytochemistry-Immunofluorescence-NBP2-67020-img0005.jpg)
![Immunohistochemistry-Paraffin: Cytochrome P450 2D6 Antibody (JM44-23) [NBP2-67020] - Analysis of paraffin-embedded human liver tissue using anti-Cytochrome P450 2D6 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/images2/Cytochrome-P450-2D6-Antibody-(JM44-23)-Immunohistochemistry-Paraffin-NBP2-67020-img0003.jpg)
![Western Blot: Cytochrome P450 1A2 Antibody (OTI8F1) [NBP2-02563] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Cytochrome P450 1A2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Cytochrome P450 1A2.](http://images.novusbio.com/images2/Cytochrome-P450-1A2-Antibody-(OTI8F1)-Western-Blot-NBP2-02563-img0016.jpg)
![Immunocytochemistry/Immunofluorescence: Cytochrome P450 1A2 Antibody (OTI8F1) [NBP2-02563] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY CYP1A2.](http://images.novusbio.com/images2/Cytochrome P450 1A2 Antibody (OTI8F1)-Immunocytochemistry-Immunofluorescence-NBP2-02563-img0017.jpg)
![Immunohistochemistry-Paraffin: Cytochrome P450 1A2 Antibody (OTI8F1) [NBP2-02563] - Staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-Cytochrome P450 1A2 mouse monoclonal antibody.](http://images.novusbio.com/images2/Cytochrome-P450-1A2-Antibody-(OTI8F1)-Immunohistochemistry-Paraffin-NBP2-02563-img0015.jpg)
![Western Blot: Cytochrome P450 2B6 Antibody (3D5) [NBP2-01800] - Analysis of extracts (35ug) from 9 different cell lines by using anti-Cytochrome P450 2B6 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).](http://images.novusbio.com/images2/Cytochrome-P450-2B6-Antibody-(3D5)-Western-Blot-NBP2-01800-img0026.jpg)
![Immunocytochemistry/Immunofluorescence: Cytochrome P450 2B6 Antibody (3D5) [NBP2-01800] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY Cytochrome P450 2B6.](http://images.novusbio.com/images2/Cytochrome-P450-2B6-Antibody-(3D5)-Immunocytochemistry-Immunofluorescence-NBP2-01800-img0015.jpg)
![Immunohistochemistry-Paraffin: Cytochrome P450 2B6 Antibody (3D5) [NBP2-01800] - Staining of paraffin-embedded Human liver tissue using anti-Cytochrome P450 2B6 mouse monoclonal antibody.](http://images.novusbio.com/images2/Cytochrome-P450-2B6-Antibody-(3D5)-Immunohistochemistry-Paraffin-NBP2-01800-img0024.jpg)
![Immunocytochemistry/Immunofluorescence: AHR Antibody [NB100-128] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton X-100. The cells were incubated with anti AHR [NB100-128] at a 1:100 dilution overnight at 4C and detected with an anti-goat DyLight 488 (Green) at a 1:500 dilution. Actin was detected with Phalloidin 568 (Red) at a 1:200 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/images2/AHR-Antibody-Immunocytochemistry-Immunofluorescence-NB100-128-img0004.jpg)
![Immunohistochemistry-Paraffin: AHR Antibody [NB100-128] - Analysis of FFPE human liver using 20 ug/ml conc. of AHR antibody on a Bond Rx autostainer (Leica Biosystems). The assay involved 20 minutes of heat induced antigen retrieval (HIER) using 10mM sodium citrate buffer (pH 6.0) and endogenous peroxidase quenching with peroxide block. The sections were incubated with primary antibody for 30 minutes and Bond Polymer Refine Detection (Leica Biosystems) with DAB was used for signal development followed by counterstaining with hematoxylin. Whole slide scanning and capturing of representative images was performed using Aperio AT2 (Leica Biosystems). Staining was performed by Histowiz.](http://images.novusbio.com/images2/AHR-Antibody-Immunohistochemistry-Paraffin-NB100-128-img0006.jpg)
![Gel Super Shift Assays: AHR Antibody [NB100-128] - EMSA validation of AHR antibody on U937 cells nuclear protein. Image submitted by verified customer - see review for protocol details.](http://images.novusbio.com/images2/AHR-Antibody-Gel-Super-Shift-Assays-NB100-128-img0003.jpg)
![Western Blot: CYP3A7 Antibody [NBP1-62387] - Human Liver cell lysate, concentration 2.5 ug/ml.](http://images.novusbio.com/images2/CYP3A7 Antibody-Western Blot-NBP1-62387-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: TBC1D9 Antibody [NBP2-13415] - Staining of human cell line U-2 OS shows localization to nucleoplasm & cytosol.](http://images.novusbio.com/images2/TBC1D9-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-13415-img0003.jpg)
![Immunohistochemistry-Paraffin: TBC1D9 Antibody [NBP2-13415] - Staining of human testis shows moderate cytoplasmic positivity in cells in seminiferous ducts.](http://images.novusbio.com/images2/TBC1D9 Antibody-Immunohistochemistry-Paraffin-NBP2-13415-img0004.jpg)
![Western Blot: Cytochrome P450 2A6 Antibody (OTI1D2) [NBP2-01437] - Analysis of extracts (35ug) from 9 different cell lines by using anti-Cytochrome P450 2A6 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).](http://images.novusbio.com/images2/Cytochrome-P450-2A6-Antibody-(OTI1D2)-Western-Blot-NBP2-01437-img0009.jpg)
![Immunohistochemistry-Paraffin: Cytochrome P450 2A6 Antibody (OTI1D2) [NBP2-01437] - Staining of paraffin-embedded Human prostate tissue using anti-Cytochrome P450 2A6 mouse monoclonal antibody.](http://images.novusbio.com/images2/Cytochrome-P450-2A6-Antibody-(OTI1D2)-Immunohistochemistry-Paraffin-NBP2-01437-img0015.jpg)
![Flow Cytometry: Cytochrome P450 2A6 Antibody (OTI1D2) [NBP2-01437] - Analysis of Jurkat cells, using anti-CYP2A6 antibody, (Red), compared to a nonspecific negative control antibody, (Blue).](http://images.novusbio.com/images2/Cytochrome P450 2A6 Antibody (OTI1D2)-Flow Cytometry-NBP2-01437-img0016.jpg)
![Western Blot: Cytochrome P450 2C8 Antibody [NBP1-88054] - Analysis using Anti-CYP2C8 antibody NBP1-88054 (A) shows similar pattern to independent antibody NBP1-88055 (B).](http://images.novusbio.com/images2/Cytochrome-P450-2C8-Antibody-Western-Blot-NBP1-88054-img0009.jpg)
![Immunohistochemistry-Paraffin: Cytochrome P450 2C8 Antibody [NBP1-88054] - Staining of human cerebellum, fallopian tube, liver and skin using Anti-CYP2C8 antibody (A) NBP1-88054 shows similar protein distribution across tissues to independent antibody NBP1-88055 (B).](http://images.novusbio.com/images2/Cytochrome P450 2C8 Antibody-Immunohistochemistry-Paraffin-NBP1-88054-img0018.jpg)
![Immunohistochemistry-Paraffin: Cytochrome P450 2C8 Antibody [NBP1-88054] - Analysis in human liver and skin tissues. Corresponding CYP2C8 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/images2/Cytochrome-P450-2C8-Antibody-Immunohistochemistry-Paraffin-NBP1-88054-img0013.jpg)
![Western Blot: Cytochrome P450 2E1 Antibody [NBP1-85367] - Western blot analysis in mouse liver tissue and rat liver tissue.](http://images.novusbio.com/images2/Cytochrome-P450-2E1-Antibody-Western-Blot-NBP1-85367-img0011.jpg)
![Immunohistochemistry-Paraffin: Cytochrome P450 2E1 Antibody [NBP1-85367] - Staining of human endometrium shows no positivity in glandular or stromal cells as expected.](http://images.novusbio.com/images2/Cytochrome-P450-2E1-Antibody-Immunohistochemistry-Paraffin-NBP1-85367-img0023.jpg)
![Western Blot: Cytochrome P450 2E1 Antibody [NBP1-85367] - Analysis in human liver tissue.](http://images.novusbio.com/images2/Cytochrome-P450-2E1-Antibody-Western-Blot-NBP1-85367-img0009.jpg)
![Western Blot: PXR/NR1I2 Antibody (1D12G1) [NBP2-61816] - Analysis using NR1I2 mAb against human NR1I2 (AA: 1-142) recombinant protein. (Expected MW is 42.2 kDa)](http://images.novusbio.com/images2/PXR-NR1I2-Antibody-(1D12G1)-Western-Blot-NBP2-61816-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: PXR/NR1I2 Antibody (1D12G1) [NBP2-61816] - Analysis of Hela cells using NR1I2 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Goat anti-Mouse IgG (H+L) DyLight 488 Secondary antibody was used.](http://images.novusbio.com/images2/PXR-NR1I2-Antibody-(1D12G1)-Immunocytochemistry-Immunofluorescence-NBP2-61816-img0003.jpg)
![Western Blot: PXR/NR1I2 Antibody (1D12G1) [NBP2-61816] - Analysis using NR1I2 mAb against HEK293 (1) and NR1I2 (AA: 1-142)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/images2/PXR-NR1I2 Antibody (1D12G1)-Western Blot-NBP2-61816-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: Histatin 3 Antibody (4G9) [H00003347-M01] - Analysis of monoclonal antibody to HTN3 on HeLa cell. Antibody concentration 10 ug/ml.](http://images.novusbio.com/images2/Histatin-3-Antibody-(4G9)-Immunocytochemistry-Immunofluorescence-H00003347-M01-img0005.jpg)
![Sandwich ELISA: Histatin 3 Antibody (4G9) [H00003347-M01] - Detection limit for recombinant GST tagged HTN3 is approximately 3ng/ml as a capture antibody.](http://images.novusbio.com/images2/Histatin-3-Antibody-(4G9)-Sandwich-ELISA-H00003347-M01-img0006.jpg)
![Flow Cytometry: Mouse, Human IgG1 Kappa Light Chain Isotype Control (P3.6.2.8.1) [NBP1-43319] - Mouse IgG1 Kappa Light Chain Isotype Control (P3.6.2.8.1) [NBP1-43319] - Analysis of Involucrin signal in human keratinocytes. Red: anti-IVL antibody. BLACK: Isotype control NBP1-43319APC at 1:100. Image from verified customer review.](http://images.novusbio.com/images2/Mouse IgG1 Kappa Light Chain Isotype Control (P3.6.2.8.1)-Flow Cytometry-NBP1-43319-img0004.jpg "Flow Cytometry: Mouse, Human IgG1 Kappa Light Chain Isotype Control (P3.6.2.8.1) [NBP1-43319] - Mouse IgG1 Kappa Light Chain Isotype Control (P3.6.2.8.1) [NBP1-43319] - Analysis of Involucrin signal in human keratinocytes. Red: anti-IVL antibody. BLACK: Isotype control NBP1-43319APC at 1:100. Image from verified customer review.")