Cytochrome P450 3A4 Antibody

Western Blot: Cytochrome P450 3A4 Antibody [H00001576-D01P] - Analysis of CYP3A4 expression in HepG2.

Western Blot: Cytochrome P450 3A4 Antibody [H00001576-D01P] - Analysis of CYP3A4 expression in transfected 293T cell line by CYP3A4 polyclonal antibody.Lane 1: CYP3A4 transfected lysate(57.30 KDa).Lane 2: Non-transfected lysate.

Product Discontinued

Cytochrome P450 3A4 Antibody Summary

• Description: Quality control test: Antibody reactive against mammalian transfected lysate.
• Immunogen: CYP3A4 (AAI01632.1, 1 a.a. - 503 a.a.) full-length human protein. MALIPDLAMETWLLLAVSLVLLYLYGTHSHGLFKKLGIPGPTPLPFLGNILSYHKGFCMFDMECHKKYGKVWGFYDGQQPVLAITDPDMIKTVLVKECYSVFTNRRPFGPVGFMKSAISIAEDEEWKRLRSLLSPTFTSGKLKEMVPIIAQYGDVLVRNLRREAETGKPVTLKDVFGAYSMDVITSTSFGVNIDSLNNPQDPFVENTKKLLRFDFLDPFFLSITVFPFLIPILEVLNICVFPREVTNFLRKSVKRMKESRLEDTQKHRVDFLQLMIDSQNSKETESHKALSDLELVAQSIIFIFAGYETTSSVLSFIMYELATHPDVQQKLQEEIDAVLPNKAPPTYDTVLQMEYLDMVVNETLRLFPIAMRLERVCKKDVEINGMFIPKGVVVMIPSYALHRDPKYWTEPEKFLPERFSKKNKDNIDPYIYTPFGSGPRNCIGMRFALMNMKLALIRVLQNFSFKPCKETQIPLKLSLGGLLQPEKPVVLKVESRDGTVSGA
• Specificity: CYP3A4 - cytochrome P450, family 3, subfamily A, polypeptide 4,
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: CYP3A4
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Western Blot
• Application Notes: Antibody reactivity against Recombinant Protein with GST tag on ELISA and WB and also on transfected lysate in WB. GST tag alone is used as a negative control.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.4)
• Preservative: No Preservative
• Purity: Protein A purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for Cytochrome P450 3A4 Antibody

• Albendazole Monooxygenase
• Albendazole Sulfoxidase
• CP33
• CP34
• CYP3A
• CYP3A3
• CYP3A4
• CYPIIIA3
• CYPIIIA4
• Cytochrome P450 3A3
• cytochrome P450 3A4
• Cytochrome P450 HLp
• Cytochrome P450 NF-25
• cytochrome P450, family 3, subfamily A, polypeptide 4
• cytochrome P450, subfamily IIIA (niphedipine oxidase), polypeptide 3
• cytochrome P450, subfamily IIIA (niphedipine oxidase), polypeptide 4
• Cytochrome P450-PCN1
• EC 1.14.13.32
• EC 1.14.13.67
• EC 1.14.13.97
• EC 1.14.14.1
• glucocorticoid-inducible P450
• HLP
• MGC126680
• NF-25
• Nifedipine oxidase
• P450C3
• P450-III, steroid inducible
• P450PCN1
• Quinine 3-Monooxygenase
• Taurochenodeoxycholate 6-Alpha-Hydroxylase

Background

This gene, CYP3A4, encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the endoplasmic reticulum and its expression is induced by glucocorticoids and some pharmacological agents. This enzyme is involved in the metabolism of approximately half the drugs which are are used today, including acetaminophen, codeine, cyclosporin A, diazepam and erythromycin. The enzyme also metabolizes some steroids and carcinogens. This gene is part of a cluster of cytochrome P450 genes on chromosome 7q21.1. Previously another CYP3A gene, CYP3A3, was thought to exist; however, it is now thought that this sequence represents a transcript variant of CYP3A4. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Cytochrome P450 3A4 Antibody (H00001576-D01P)(1)

Publication using H00001576-D01P

• De Bock L, Colin P, Boussery K, Van Bocxlaer J. Development and validation of an enzyme-linked immunosorbent assay for the quantification of cytochrome 3A4 in human liver microsomes. Talanta 64. 2012-01-01 [PMID: 22967564]

Bioinformatics

• Gene Symbol: CYP3A4
• Entrez:
  • 1576(Human)
• Uniprot:
  • AAI01632.1(Human)