Cytochrome P450 3A4 Antibody

Western Blot: Cytochrome P450 3A4 Antibody [H00001576-B01P] - Analysis of CYP3A4 expression in human liver.

Immunohistochemistry-Paraffin: Cytochrome P450 3A4 Antibody [H00001576-B01P] - Analysis of purified antibody to CYP3A4 on formalin-fixed paraffin-embedded human small Intestine. (antibody concentration 3 ug/ml)

Western Blot: Cytochrome P450 3A4 Antibody [H00001576-B01P] - Analysis of CYP3A4 expression in HepG2.

Western Blot: Cytochrome P450 3A4 Antibody [H00001576-B01P] - Analysis of CYP3A4 expression in transfected 293T cell line by CYP3A4 polyclonal antibody. Lane 1: CYP3A4 transfected lysate(55.33 KDa). Lane 2: Non-transfected lysate.

Product Discontinued

Cytochrome P450 3A4 Antibody Summary

• Description: Quality control test: Antibody reactive against mammalian transfected lysate.
• Immunogen: CYP3A4 (AAI01632.1, 1 a.a. - 503 a.a.) full-length human protein. MALIPDLAMETWLLLAVSLVLLYLYGTHSHGLFKKLGIPGPTPLPFLGNILSYHKGFCMFDMECHKKYGKVWGFYDGQQPVLAITDPDMIKTVLVKECYSVFTNRRPFGPVGFMKSAISIAEDEEWKRLRSLLSPTFTSGKLKEMVPIIAQYGDVLVRNLRREAETGKPVTLKDVFGAYSMDVITSTSFGVNIDSLNNPQDPFVENTKKLLRFDFLDPFFLSITVFPFLIPILEVLNICVFPREVTNFLRKSVKRMKESRLEDTQKHRVDFLQLMIDSQNSKETESHKALSDLELVAQSIIFIFAGYETTSSVLSFIMYELATHPDVQQKLQEEIDAVLPNKAPPTYDTVLQMEYLDMVVNETLRLFPIAMRLERVCKKDVEINGMFIPKGVVVMIPSYALHRDPKYWTEPEKFLPERFSKKNKDNIDPYIYTPFGSGPRNCIGMRFALMNMKLALIRVLQNFSFKPCKETQIPLKLSLGGLLQPEKPVVLKVESRDGTVSGA
• Specificity: CYP3A4 - cytochrome P450, family 3, subfamily A, polypeptide 4,
• Isotype: IgG
• Clonality: Polyclonal
• Host: Mouse
• Gene: CYP3A4
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Western Blot
• Application Notes: Antibody reactivity against Recombinant Protein with GST tag on ELISA and WB and also on transfected lysate in WB. GST tag alone is used as a negative control. It has been used for IHC-P.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.4)
• Preservative: No Preservative
• Purity: Protein A purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for Cytochrome P450 3A4 Antibody

• Albendazole Monooxygenase
• Albendazole Sulfoxidase
• CP33
• CP34
• CYP3A
• CYP3A3
• CYP3A4
• CYPIIIA3
• CYPIIIA4
• Cytochrome P450 3A3
• cytochrome P450 3A4
• Cytochrome P450 HLp
• Cytochrome P450 NF-25
• cytochrome P450, family 3, subfamily A, polypeptide 4
• cytochrome P450, subfamily IIIA (niphedipine oxidase), polypeptide 3
• cytochrome P450, subfamily IIIA (niphedipine oxidase), polypeptide 4
• Cytochrome P450-PCN1
• EC 1.14.13.32
• EC 1.14.13.67
• EC 1.14.13.97
• EC 1.14.14.1
• glucocorticoid-inducible P450
• HLP
• MGC126680
• NF-25
• Nifedipine oxidase
• P450C3
• P450-III, steroid inducible
• P450PCN1
• Quinine 3-Monooxygenase
• Taurochenodeoxycholate 6-Alpha-Hydroxylase

Background

This gene, CYP3A4, encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the endoplasmic reticulum and its expression is induced by glucocorticoids and some pharmacological agents. This enzyme is involved in the metabolism of approximately half the drugs which are are used today, including acetaminophen, codeine, cyclosporin A, diazepam and erythromycin. The enzyme also metabolizes some steroids and carcinogens. This gene is part of a cluster of cytochrome P450 genes on chromosome 7q21.1. Previously another CYP3A gene, CYP3A3, was thought to exist; however, it is now thought that this sequence represents a transcript variant of CYP3A4. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Cytochrome P450 3A4 Antibody (H00001576-B01P)(4)

Publications using H00001576-B01P

• Niu Y, Fan S, Luo Q et al. Interaction of Hepatitis B Virus X Protein with the Pregnane X Receptor Enhances the Synergistic Effects of Aflatoxin B1 and Hepatitis B Virus on Promoting Hepatocarcinogenesis J Clin Transl Hepatol 2021-08-27 [PMID: 34447675]
• Wiegand C, Hewitt NJ, Merk HF, Reisinger K. Dermal Xenobiotic Metabolism: A Comparison between Native Human Skin, Four in vitro Skin Test Systems and a Liver System. Skin Pharmacol Physiol. 2014-06-13 [PMID: 24943921]
• Fahrmayr C, Konig J, Auge D et al. Phase I and II metabolism and MRP2-mediated export of bosentan in a MDCKII-OATP1B1-CYP3A4-UGT1A1-MRP2 quadruple-transfected cell line. Br J Pharmacol. 2012-12-16 [PMID: 23387445]
• Yamashita T, Honda M, Nio K et al. Oncostatin M Renders Epithelial Cell Adhesion Molecule-Positive Liver Cancer Stem Cells Sensitive to 5-Fluorouracil by Inducing Hepatocytic Differentiation. Cancer Res. 2010-05-18 [PMID: 20484035]

Bioinformatics

• Gene Symbol: CYP3A4
• Entrez:
  • 1576(Human)
• Uniprot:
  • AAI01632.1(Human)