Cystatin F Antibody (292103) [Alexa Fluor® 405]

• Reactivity: Hu
• Applications: WB, IP
• Clone: 292103
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Alexa Fluor 405

Cystatin F Antibody (292103) [Alexa Fluor® 405] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human Cystatin F
  Gly20-His145
  Accession # O76096
• Specificity: Detects human Cystatin F in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human (rh) Cystatin A, B, C, D, E/M, S, recombinant mouse (rm) Cystatin/Stefin Homolog, rhFetuin A, r
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Immunoprecipitation
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Cystatin F Antibody (292103) [Alexa Fluor® 405]

• CMAP
• CST7
• Cystatin 7
• cystatin F (leukocystatin)
• Cystatin F
• Cystatin-7
• cystatin-F
• Cystatin-like metastasis-associated protein
• Leukocystatin

Background

Cystatin F, also known as leukocystatin and CMAP (Cystatin-like Metastasis-Associated Protein), is a member of the cystatin superfamily (1‑3). Cystatin F is selectively expressed by hematopoietic cells and may be a biomarker for both liver metastasis and inflammatory lung disorders (3, 4). As a cysteine protease inhibitor, it shows selectivity towards cathepsin L and legumain (1, 2, 5). Compared to other secreted cystatins including C, D, E/M, S, SA and SN, which contain two intra disulfide bonds, cystatin F has two extra Cys residues that may be involved in inter disulfide bonds. Indeed, rhCystatin F showed disulfide bond-linked dimer formation, which was also the case for an E. coli expressed fusion protein containing mature human cystatin F and glutathione S-transferase (2).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.