Cystatin F Antibody (292103)

• Reactivity: Hu
• Applications: WB, IP
• Clone: 292103
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

Cystatin F Antibody (292103) Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human Cystatin F
  Gly20-His145
  Accession # O76096
• Specificity: Detects human Cystatin F in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human (rh) Cystatin A, B, C, D, E/M, S, recombinant mouse (rm) Cystatin/Stefin Homolog, rhFetuin A, rhFetuin B, rhHPRG, rhKininogen, or rhKininostatin is observed.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: CST7
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunoprecipitation 25 ug/mL
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Cystatin F Antibody (292103)

• CMAP
• CST7
• Cystatin 7
• cystatin F (leukocystatin)
• Cystatin F
• Cystatin-7
• cystatin-F
• Cystatin-like metastasis-associated protein
• Leukocystatin

Background

Cystatin F, also known as leukocystatin and CMAP (Cystatin-like Metastasis-Associated Protein), is a member of the cystatin superfamily (1-3). Cystatin F is selectively expressed by hematopoietic cells and may be a biomarker for both liver metastasis and inflammatory lung disorders (3, 4). As a cysteine protease inhibitor, it shows selectivity towards cathepsin L and legumain (1, 2, 5). Compared to other secreted cystatins including C, D, E/M, S, SA and SN, which contain two intra disulfide bonds, cystatin F has two extra Cys residues that may be involved in inter disulfide bonds. Indeed, rhCystatin F showed disulfide bond-linked dimer formation, which was also the case for an E. coli expressed fusion protein containing mature human cystatin F and glutathione S-transferase (2).

1. Ni, J. et al. (1998) J. Biol. Chem. 273:24797.
2. Halfon, S. et al. (1998) J. Biol. Chem. 273:16400.
3. Utsunomiya, T. et al. (2002) Clin Cancer 8:2591
4. Werle, B. et al. (2003) Biol. Chem. 384:281.
5. Gruninger-Leitch, F. et al. (2000) Nat. Biotechnol. 18:66.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CST7
• Uniprot:
  • O76096()