Cystatin B/Stefin B Antibody

Immunohistochemistry-Paraffin: Cystatin B/Stefin B Antibody [NBP2-49285] - Staining of human stomach shows low expression as expected.

Immunohistochemistry-Paraffin: Cystatin B/Stefin B Antibody [NBP2-49285] - Staining of human esophagus shows high expression.

Immunohistochemistry-Paraffin: Cystatin B/Stefin B Antibody [NBP2-49285] - Staining in human esophagus and stomach tissues using anti-CSTB antibody. Corresponding CSTB RNA-seq data are presented for the same tissues.

• Reactivity: Hu
• Applications: IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

Cystatin B/Stefin B Antibody Summary

• Immunogen: This antibody was developed against a recombinant protein corresponding to amino acids: MMCGAPSATQPATAETQHIADQ
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: CSTB
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:50 - 1:200
  • Immunohistochemistry-Paraffin 1:50 - 1:200
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended.
• Control Peptide:

  Cystatin B/Stefin B Recombinant Protein Antigen (NBP2-49285PEP)

Reactivity Notes

Mouse (86%), Rat (82%)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for Cystatin B/Stefin B Antibody

• CPI-B
• CST6cystatin B (liver thiol proteinase inhibitor)10STFBcystatin-B
• CSTB
• cystatin B (stefin B)
• Cystatin B
• EPM1
• Liver thiol proteinase inhibitor
• PME
• Stefin B
• stefin-B

Background

The cystatin superfamily encompasses proteins that contain multiple cystatin-like sequences. Some of the members are active cysteine protease inhibitors, while others have lost or perhaps never acquired this inhibitory activity. There are three inhibitory families in the superfamily, including the type 1 cystatins (stefins), type 2 cystatins and kininogens. This gene encodes a stefin that functions as an intracellular thiol protease inhibitor. The protein is able to form a dimer stabilized by noncovalent forces, inhibiting papain and cathepsins l, h and b. The protein is thought to play a role in protecting against the proteases leaking from lysosomes. Evidence indicates that mutations in this gene are responsible for the primary defects in patients with progressive myoclonic epilepsy (EPM1).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CSTB