CYP27A1 Antibody (JG40-36)

Western Blot: CYP27A1 Antibody (JG40-36) [NBP2-76827] - Analysis of CYP27A1 on different tissue lysates using anti-CYP27A1 antibody at 1:500 dilution. Positive control: Lane 1: Rat heart. Lane 2: Human liver. Lane 2: Human kidney.

Immunocytochemistry/Immunofluorescence: CYP27A1 Antibody (JG40-36) [NBP2-76827] - ICC staining CYP27A1 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X-100/PBS.

Immunohistochemistry: CYP27A1 Antibody (JG40-36) [NBP2-76827] - Immunohistochemical analysis of paraffin-embedded human small intestine tissue using anti-CYP27A1 antibody. Counter stained with hematoxylin.

Flow Cytometry: CYP27A1 Antibody (JG40-36) [NBP2-76827] - Flow cytometric analysis of HepG2 cells with CYP27A1 antibody at 1/100 dilution (purple) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.

Immunocytochemistry/Immunofluorescence: CYP27A1 Antibody (JG40-36) [NBP2-76827] - Staining of CYP27A1 in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X-100/PBS.

Immunohistochemistry: CYP27A1 Antibody (JG40-36) [NBP2-76827] - Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using anti-CYP27A1 antibody. Counter stained with hematoxylin.

Immunohistochemistry: CYP27A1 Antibody (JG40-36) [NBP2-76827] - Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-CYP27A1 antibody. Counter stained with hematoxylin.

Product Discontinued

CYP27A1 Antibody (JG40-36) Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Recombinant protein within Human CYP27A1 aa 400-531 / 531. (SwissProt: Q02318 Human; SwissProt: Q9DBG1 Mouse; SwissProt: P17178 Rat)
• Localization: Mitochondrion membrane.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: CYP27A1
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:50-1:100
  • Immunocytochemistry/ Immunofluorescence 1:50-1:200
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Western Blot 1:500-1:2000
• Theoretical MW: 60 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: TBS (pH7.4), 0.05% BSA, 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for CYP27A1 Antibody (JG40-36)

• 12-alpha-triol 27-hydroxylase
• 7-alpha
• cholestanetriol 26-monooxygenase
• CP27,5-beta-cholestane-3-alpha, 7-alpha, 12-alpha-triol 27-hydroxylase
• CTX5-beta-cholestane-3-alpha
• CYP27
• CYP275-beta-cholestane-3-alpha, 7-alpha, 12-alpha-triol 26-hydroxylase
• CYP27A1
• Cytochrome P450 27
• cytochrome P450, family 27, subfamily A, polypeptide 1
• cytochrome P450, subfamily XXVIIA (steroid 27-hydroxylase, cerebrotendinousxanthomatosis), polypeptide 1
• Cytochrome P-450C27/25
• EC 1.14.13.15
• EC 1.14.15.15
• sterol 26-hydroxylase, mitochondrial
• Sterol 27-hydroxylase
• Vitamin D(3) 25-hydroxylase

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CYP27A1