Cyclophilin-F Antibody

Western Blot: E coli-derived fusion protein as test antigen. Affinity-purified IgY dilution: 1:2000, Goat anti-IgY-HRP dilution: 1:1000. Colorimetric method for signal development. ( anti-PPIF )

ELISA: Cyclophilin-F Antibody [NB100-75452] - Direct Elisa Test. Protein as test antigen. Affi-pure IgY as primary antibody and Goat anti-IgY HRP as 2nd antibody. Fixed amount of antibody (1 ug/ml) and serial dilutions of antigen. ( anti-PPIF )

Product Discontinued

Cyclophilin-F Antibody Summary

• Immunogen: The immunogen for this antibody was a sequence from 1a.a. to 207a.a. from Cyclophilin F(a 207a.a. long protein)
• Localization: Mitochondrial; mitochondrial matrix.
• Specificity: Cyclophilin-F
• Clonality: Polyclonal
• Host: Chicken
• Gene: PPIF
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Western Blot
• Application Notes: ELISA: Direct Elisa Test. Protein as test antigen. Affi-pure IgY as primary antibody and Goat anti-IgY HRP as 2nd antibody. Fixed amount of antibody (1 ug/ml) and serial dilutions of antigen.

Reactivity Notes

This antibody is reactive against Human, mouse, rat.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: No Preservative
• Purity: Immunogen affinity purified

Notes

Chicken products cannot be exported to Canada.

Alternate Names for Cyclophilin-F Antibody

• Cyclophilin F
• CYP3FLJ90798
• Cyp-D
• hCyP3
• MGC117207
• mitochondrial
• peptidyl-prolyl cis-trans isomerase, mitochondrial
• peptidylprolyl isomerase F (cyclophilin F)
• peptidylprolyl isomerase F

Background

The protein encoded by this gene is a member of the peptidyl-prolyl cis-trans isomerase (PPIase) family. PPIases catalyze the cis-trans isomerization of proline imidic peptide bonds in oligopeptides and accelerate the folding of proteins. This protein is part of the mitochondrial permeability transition pore in the inner mitochondrial membrane. Activation of this pore is thought to be involved in the induction of apoptotic and necrotic cell death.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PPIF
• Entrez:
  • 10105(Human)
  • 105675(Mouse)
  • 282819(Rat)
• Uniprot:
  • P30405(Human)