Cyclophilin-F Antibody (1F5) - BSA Free

Western Blot: Cyclophilin-F Antibody (1F5) [NBP1-28608] - The cell lysates(40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human PPIF antibody at a dilution of (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.Lane 1.: 293T cell lysateLane 2.: HepG2 cell lysateLane 3.: MCF7 cell lysateLane 4.: PC3 cell lysate

Immunocytochemistry/Immunofluorescence: Cyclophilin-F Antibody (1F5) [NBP1-28608] - Analysis of PPIF in HeLa cells. The cell was stained at (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).

Immunohistochemistry-Paraffin: Cyclophilin-F Antibody (1F5) [NBP1-28608] - Paraffin embedded sections of human breast cancer tissue were incubated with anti-human PPIF (1:50) for 2 hours at room temperature. Antigen retrieval was performed in 0.1M sodium citrate buffer and detected using Diaminobenzidine (DAB).

Immunohistochemistry-Paraffin: Cyclophilin-F Antibody (1F5) [NBP1-28608] - Paraffin embedded sections of human breast cancer tissue were incubated with anti-human PPIF (1:50) for 2 hours at room temperature. Antigen retrieval was performed in 0.1M sodium citrate buffer and detected using Diaminobenzidine (DAB).

• Reactivity: Hu
• Applications: WB, ELISA, ICC/IF, IHC
• Clone: 1F5
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

Cyclophilin-F Antibody (1F5) - BSA Free Summary

• Description: Novus Biologicals Mouse Cyclophilin-F Antibody (1F5) - BSA Free (NBP1-28608) is a monoclonal antibody validated for use in IHC, WB, ELISA and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant human Cyclophilin-F (30-207aa) purified from E. coli (NP_005720).
• Isotype: IgG2b Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: PPIF
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Immunohistochemistry 1:50-1:100
  • Immunohistochemistry-Paraffin 1:50-1:100
  • Western Blot 1:500-1:1000
• Theoretical MW: 22 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.4), 10% Glycerol
• Preservative: 0.02% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Protein G purified

Alternate Names for Cyclophilin-F Antibody (1F5) - BSA Free

• Cyclophilin F
• CYP3FLJ90798
• Cyp-D
• hCyP3
• MGC117207
• mitochondrial
• peptidyl-prolyl cis-trans isomerase, mitochondrial
• peptidylprolyl isomerase F (cyclophilin F)
• peptidylprolyl isomerase F

Background

The protein encoded by this gene is a member of the peptidyl-prolyl cis-trans isomerase (PPIase) family. PPIases catalyze the cis-trans isomerization of proline imidic peptide bonds in oligopeptides and accelerate the folding of proteins. This protein is part of the mitochondrial permeability transition pore in the inner mitochondrial membrane. Activation of this pore is thought to be involved in the induction of apoptotic and necrotic cell death.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PPIF
• Entrez:
  • 10105(Human)
• Uniprot:
  • P30405(Human)