CYB5R4 Overexpression Lysate (Native)

Images

 
Western Blot: CYB5R4 Overexpression Lysate (Adult Normal) [NBL1-09656] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for CYB5R4.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

CYB5R4 Overexpression Lysate (Native) Summary

Immunogen
The lysate was created in HEK293T cells, using plasmid ID RC205203 and based on accession number NM_016230. The protein contains a C-terminal DDK tag.
Specificity
Homo sapiens cytochrome b5 reductase 4 (CYB5R4), mRNA.
Gene
CYB5R4

Applications/Dilutions

Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Theoretical MW
59.3 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for CYB5R4

Type
Overexpression
Protein State
Native

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for CYB5R4 Overexpression Lysate (Native)

  • b5+b5R
  • cb5/cb5R
  • cytochrome b5 reductase 4
  • cytochrome b-type NAD(P)H oxidoreductase
  • dJ676J13.1
  • EC 1.6.2.2
  • Flavohemoprotein b5/b5R
  • flavohemoprotein b5+b5R
  • NADPH cytochrome B5 oxidoreductase
  • NCB5ORN-terminal cytochrome b5 and cytochrome b5 oxidoreductase domain-containingprotein
  • RP4-676J13.1

Background

NCB5OR is a flavohemoprotein that contains functional domains found in both cytochrome b5 (CYB5; MIM 250790) and CYB5 reductase (DIA1; MIM 250800).[supplied by OMIM].

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Customers Who Viewed This Item Also Viewed...

NB600-1335
Species: Hu, Mu, Rt, Pm
Applications: WB, Simple Western, GS, ICC/IF, IHC, IHC-P, IP
NBP2-49284
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
NBP1-06274
Species: Hu, Mu, Rt, Ch, Dr, Sh
Applications: WB, Simple Western, Flow, ICC/IF
AF1543
Species: Hu
Applications: WB, IHC
NB100-80861
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
NB100-56503
Species: Hu, Mu, Rt, Po, Ca, Dr, Eq, Ma, Rb
Applications: WB, Flow, ICC/IF, IHC, IHC-P
MAB2476
Species: Hu
Applications: WB, IHC
NBP2-34260
Species: Hu, Mu, Po, Bv, Rb
Applications: WB, Flow, ICC/IF, IHC-Fr, IHC-P, IP, PAGE
NB300-749
Species: Hu, Mu, Rt, Pm
Applications: WB, ELISA, ICC/IF, IHC, IHC-P, IP
AF7550
Species: Hu
Applications: WB
NB100-182
Species: Hu, Mu, Ca, Pm
Applications: WB, Simple Western, Flow, ICC/IF, IHC, IHC-P, IP, RNAi
NB600-335
Species: Hu, Mu, Ec, NA
Applications: WB, ELISA, ICC/IF, IHC-Fr, IP
NBP2-43648
Species: Hu, Mu, Rt, Ze
Applications: WB, ICC/IF, IHC, IHC-P
NBP1-85816
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
NB300-221
Species: Hu, Mu, Rt, Po, Av, Bv, Ch, Eq, Ha, Op, Pm, Sh, Ze
Applications: WB, Simple Western, ChIP, ICC/IF, IHC, IP
NBP2-02705
Species: Hu, Ca, Pm
Applications: WB, Flow, IHC, IHC-P
NBP2-45897
Species: Hu
Applications: WB, IHC

Publications for CYB5R4 Lysate (NBL1-09656) (0)

There are no publications for CYB5R4 Lysate (NBL1-09656).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for CYB5R4 Lysate (NBL1-09656) (0)

There are no reviews for CYB5R4 Lysate (NBL1-09656). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen
  • 1st to review with an image -- $50/€35/£30/$50 CAD/¥300 Yuan/¥5000 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for CYB5R4 Lysate (NBL1-09656) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Additional CYB5R4 Products

Bioinformatics Tool for CYB5R4 Lysate (NBL1-09656)

Discover related pathways, diseases and genes to CYB5R4 Lysate (NBL1-09656). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Blogs on CYB5R4

There are no specific blogs for CYB5R4, but you can read our latest blog posts.

Customers Who Bought This Also Bought

CYB5R4 Antibody
NBP1-88957
FANCD2 Antibody
NB100-182

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our CYB5R4 Overexpression Lysate (Native) and receive a gift card or discount.

Bioinformatics

Gene Symbol CYB5R4