CYB561D2 Antibody

Western Blot: CYB561D2 Antibody [NB300-913] - Staining of human liver lysate (RIPA buffer, 35 ug total protein per lane). Primary antibody (1 ug/ml) incubated for 1 hour. Detected by western blot using chemiluminescence.

• Reactivity: Hu, Mu, Rt, Bv, Ca
• Applications: WB, ELISA, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

CYB561D2 Antibody Summary

• Immunogen: Peptide with sequence C-VSNAYLYRKRIQP corresponding to C-Terminus according to NP_008953.1.
• Predicted Species: Rat (100%), Canine (100%), Bovine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: CYB561D2
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Peptide ELISA Detection limit 1:16000
  • Western Blot 1 - 3 ug/ml
• Application Notes: WB: Approx. 25 kDa band observed in human liver lysates (predicted MW of 26 kDa band according to NP_008953). Use in IHC-P reported in scientific literature (PMID 26997970).
• Control:

  CYB561D2 Overexpression Lysate

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 26997970).

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for CYB561D2 Antibody

• cytochrome b-561 domain containing 2,101F6cytochrome b561 domain-containing protein 2
• putative tumor suppressor 101F6
• Putative tumor suppressor protein 101F6
• TSP10

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CYB561D2 Antibody (NB300-913)(3)

Publications using NB300-913

• Merkowsky K, Sethi RS, Gill JP, Singh B. Fipronil induces lung inflammation in vivo and cell death in vitro. J Occup Med Toxicol. 2016-03-21 [PMID: 26997970] (IHC-P, Mouse)
• Recuenco MC, Rahman MM, Sakamoto Y et al. Functional characterization of the recombinant human tumour suppressor 101F6 protein, a cytochrome b(561) homologue. J Biochem. 2013-02-01 [PMID: 23235316]
• Lerman MI, Minna JD. The 630-kb lung cancer homozygous deletion region on human chromosome 3p21.3: identification and evaluation of the resident candidate tumor suppressor genes. The International Lung Cancer Chromosome 3p21.3 Tumor Suppressor Gene Consortium. Cancer Res 2000-11-01 [PMID: 11085536]

Bioinformatics

• Gene Symbol: CYB561D2
• Entrez:
  • 11068(Human)
  • 56368(Mouse)
  • 363137(Rat)
• Uniprot:
  • NP_008953.(Human)