CXCR7/RDC-1 Antibody (358426) [Phycoerythrin]

Human peripheral blood monocytes were stained with Mouse Anti-Human CXCR7/ RDC-1 PE-conjugated Monoclonal Antibody (Catalog # FAB42271P, filled histogram) or isotype control antibody (Catalog # IC003P, open histogram). View our protocol for Staining Membrane-associated Proteins.

Product Discontinued

CXCR7/RDC-1 Antibody (358426) [Phycoerythrin] Summary

• Immunogen: NS0 mouse myeloma cell line transfected with human CXCR7/RDC‑1
  Met1-Lys362 (Gly131Ser)
  Accession # AAA62370
• Specificity: Detects human CXCR7/RDC‑1 in direct ELISAs. In flow cytometry, reacts specifically with five distinct human CXCR7 transfectants, but does not react with their respective parental lines or mouse CXCR7 transfectants. In flow cytometry, also reacts with monocytes expressing CXCR7, but does not react with MCF-7 cells which have been reported to have surface-expressing CXCR7 using clone 11G8. Due to the conflicting reports published, use of clone 358426 may result in an underestimation of CXCR7 expression on certain cell types.
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: ACKR3
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 10 uL/10^6 cells

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.
• Buffer: Supplied in a saline solution containing BSA and Sodium Azide.
• Preservative: Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CXCR7/RDC-1 Antibody (358426) [Phycoerythrin]

• ACKR3
• chemokine (C-X-C motif) receptor 7
• Chemokine orphan receptor 1CMKOR1C-X-C chemokine receptor type 7
• CMKOR1
• CXCR7
• CXC-R7
• CXCR-7
• G protein-coupled receptor
• GPR159
• GPR159G-protein coupled receptor 159
• RDC-1
• RDC1G-protein coupled receptor RDC1 homolog

Background

The G protein-coupled receptor, RDC1, belongs to a subgroup of chemokine receptors and has been designated CXCR7. CXCR7 can bind with high-affinity to CXCL12/SDF-1 and CXCL11/I-TAC. It is also a co-receptor for several HIV and SIV strains. In their N-termini and extracellular loops 1, 2, and 3, human and mouse CXCR7 share 84%, 100%, 96% and 86% amino acid sequence identity, respectively. Reports of mRNA levels and/or protein expression (as assessed using anti‑CXCR7, clone 9C4) (1, 2) indicate that CXCR7 occurs on a wide variety of tissues and cells including monocytes, B cells, T cells and mature dendritic cells. In contrast, based on ligand binding analysis and receptor level (as assessed using anti‑CXCR7, clone 11G8), surface expression of CXCR7 was reported to be restricted to tumor cells, activated endothelial cells, fetal liver cells, and few other cell types (3). The basis of these inconsistent observations is not known but may be attributed to cell context and the use of different antibodies that may recognize different epitopes.

1. Balabanian, K. et al. (2005) J. Biol. Chem. 280:35760.
2. Infantino, S. et al. (2006) J. Immunol. 176:2197.
3. Burns, J.M. et al. (2006) J. Exp. Med. 203:2201.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CXCR7/RDC-1 Antibody (FAB42271P)(10)

Publications using FAB42271P

• M Joo, JB Heo, S Kim, N Kim, HJ Jeon, Y An, GY Song, JM Kim, HJ Lee Decursin inhibits tumor progression in head and neck squamous cell carcinoma by downregulating CXCR7 expression in�vitro Oncology reports, 2021-12-27;47(2):. 2021-12-27 [PMID: 34958113] (Flow Cytometry, Human)
• S Kim, JE Kim, N Kim, M Joo, MW Lee, HJ Jeon, H Ryu, IC Song, GY Song, HJ Lee Decursin inhibits tumor growth, migration, and invasion in gastric cancer by down-regulating CXCR7 expression Am J Cancer Res, 2019-09-01;9(9):2007-2018. 2019-09-01 [PMID: 31598401] (Flow Cytometry, Human)
• Nikolaus Heveker Mutational Analysis of Atypical Chemokine Receptor 3 (ACKR3/CXCR7) Interaction with its Chemokine Ligands CXCL11 and CXCL12 J. Biol. Chem., 2016-11-14;0(0):. 2016-11-14 [PMID: 27875312] (Flow Cytometry, Human)
• Liu Y, Carson-Walter E, Walter K Targeting chemokine receptor CXCR7 inhibits glioma cell proliferation and mobility. Anticancer Res, 2015-01-01;35(1):53-64. 2015-01-01 [PMID: 25550535] (Flow Cytometry, Human)
• Liu Y, Carson-Walter E, Walter K Chemokine receptor CXCR7 is a functional receptor for CXCL12 in brain endothelial cells. PLoS ONE, 2014-08-01;9(8):e103938. 2014-08-01 [PMID: 25084358] (Flow Cytometry, Human)
• Fekete N, Rojewski M, Furst D, Kreja L, Ignatius A, Dausend J, Schrezenmeier H GMP-compliant isolation and large-scale expansion of bone marrow-derived MSC. PLoS ONE, 2012-08-14;7(8):e43255. 2012-08-14 [PMID: 22905242] (Flow Cytometry, Human)
• Llinas L, Lazaro A, de Salort J, Matesanz-Isabel J, Sintes J, Engel P Expression profiles of novel cell surface molecules on B-cell subsets and plasma cells as analyzed by flow cytometry. Immunol. Lett., 2010-10-15;134(2):113-21. 2010-10-15 [PMID: 20951740] (Flow Cytometry, Human)
• McDermott DH, De Ravin SS, Jun HS Severe congenital neutropenia resulting from G6PC3 deficiency with increased neutrophil CXCR4 expression and myelokathexis. Blood, 2010-07-08;116(15):2793-802. 2010-07-08 [PMID: 20616219] (Flow Cytometry, Human)
• Feng Hong, Ana Tuyama, Ting Fang Lee, Johnny Loke, Ritu Agarwal, Xin Cheng et al. Hepatic stellate cells express functional CXCR4: role in stromal cell-derived factor-1alpha-mediated stellate cell activation Hepatology 6/2/2010 [PMID: 19434726]
• Feng Hong, Ana Tuyama, Ting Fang Lee, Johnny Loke, Ritu Agarwal, Xin Cheng et al. Hepatic stellate cells express functional CXCR4: role in stromal cell-derived factor-1alpha-mediated stellate cell activation Hepatology 2010-06-02 [PMID: 19434726] (Flow Cytometry, Human)

Bioinformatics

• Gene Symbol: ACKR3
• Uniprot:
  • AAA62370()