RAMP2 Antibody (773816) [Unconjugated]

bEnd.3 mouse endothelioma cell line was stained with Rat Anti-Mouse RAMP2 Monoclonal Antibody (Catalog # MAB6500, filled histogram) or isotype control antibody (Catalog # MAB006, open histogram), followed by Allophycocyanin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0113).

RAMP2 was detected in perfusion fixed frozen sections of mouse lung using Rat Anti-Mouse RAMP2 Monoclonal Antibody (Catalog # MAB6500) at 25 µg/mL overnight at 4 °C. Tissue was stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # NL013) and counterstained with DAPI (blue). Specific staining was localized to epithelial cells. View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.

• Reactivity: Mu
• Applications: Flow, IHC, CyTOF-ready
• Clone: 773816
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Unconjugated
• Concentration: LYOPH

RAMP2 Antibody (773816) [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant mouse RAMP2
  Ser45-Val159
  Accession # Q9WUP0
• Specificity: Detects mouse RAMP2 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human RAMP2 is observed.
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Rat
• Gene: RAMP2
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 0.25 ug/10^6 cells
  • Immunohistochemistry 8-25 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Sterile PBS to a final concentration of 0.5 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for RAMP2 Antibody (773816) [Unconjugated]

• calcitonin receptor-like receptor activity modifying protein 2
• Calcitonin-receptor-like receptor activity-modifying protein 2
• CRLR activity-modifying protein 2
• RAMP2
• receptor (calcitonin) activity modifying protein 2
• receptor (G protein-coupled) activity modifying protein 2
• receptor activity modifying protein 2
• receptor activity-modifying protein 2
• receptor-activity-modifying protein 2

Background

RAMP2 (receptor activity modifying protein 2) is a 20 kDa member of the RAMP family of proteins. It is expressed on cardiomyocytes, vascular smooth muscle cells and endothelium and interacts with CRLR to form a receptor complex for adrenomedullin (AM). AM induces vasodilation on AM1 receptor expressing cells. Mature mouse RAMP2 is a 145 amino acid (aa) type I transmembrane glycoprotein that contains a 115 aa extracellular domain (ECD) (aa 45-159) and a nine aa cytoplasmic region. Although the ECD contains no typical structural motifs, based on human, aa 100-106 are critical for AM binding. Over aa 45-159, mouse RAMP2 shares 57% and 83% aa identity with human and rat RAMP2, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for RAMP2 Antibody (MAB6500)(1)

Publication using MAB6500

• Iwata, S;Yoshida, R;Takai, S;Sanematsu, K;Shigemura, N;Ninomiya, Y; Adrenomedullin Enhances Mouse Gustatory Nerve Responses to Sugars via T1R-Independent Sweet Taste Pathway Nutrients 2023-01-01 [PMID: 37447268] (IHC, Mouse)

Bioinformatics

• Gene Symbol: RAMP2
• Uniprot:
  • Q9WUP0(Mouse)