CXCR7/RDC-1 Antibody (358426) [PerCP (Peridinin-chlorophyll Protein Complex)]

Human peripheral blood monocytes were stained with Mouse Anti-Human CXCR7/RDC-1 PerCP-conjugated Mono-clonal Antibody (Catalog # FAB42271C, filled histogram) or isotype control antibody (Catalog # IC003C, open histogram). View our protocol for Staining Membrane-associated Proteins.

Product Discontinued

CXCR7/RDC-1 Antibody (358426) [PerCP (Peridinin-chlorophyll Protein Complex)] Summary

• Immunogen: NS0 mouse myeloma cell line transfected with human CXCR7/RDC‑1
  Met1-Lys362 (Gly131Ser)
  Accession # AAA62370
• Specificity: Detects human CXCR7/RDC‑1 in direct ELISAs. In flow cytometry, reacts specifically with five distinct human CXCR7 transfectants, but does not react with their respective parental lines or mouse CXCR7 transfectants. In flow cytometry, also reacts with monocytes expressing CXCR7, but does not react with MCF-7 cells which have been reported to have surface-expressing CXCR7 using clone 11G8. Due to the conflicting reports published, use of clone 358426 may result in an underestimation of CXCR7 expression on certain cell types.
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: ACKR3
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 10 uL/10^6 cells

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.
• Buffer: Supplied in a saline solution containing BSA and Sodium Azide.
• Preservative: Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CXCR7/RDC-1 Antibody (358426) [PerCP (Peridinin-chlorophyll Protein Complex)]

• ACKR3
• chemokine (C-X-C motif) receptor 7
• Chemokine orphan receptor 1CMKOR1C-X-C chemokine receptor type 7
• CMKOR1
• CXCR7
• CXC-R7
• CXCR-7
• G protein-coupled receptor
• GPR159
• GPR159G-protein coupled receptor 159
• RDC-1
• RDC1G-protein coupled receptor RDC1 homolog

Background

The G protein-coupled receptor, RDC1, belongs to a subgroup of chemokine receptors and has been designated CXCR7. CXCR7 can bind with high-affinity to CXCL12/SDF-1 and CXCL11/I-TAC. It is also a co-receptor for several HIV and SIV strains. In their N-termini and extracellular loops 1, 2, and 3, human and mouse CXCR7 share 84%, 100%, 96% and 86% amino acid sequence identity, respectively. Reports of mRNA levels and/or protein expression (as assessed using anti‑CXCR7, clone 9C4) (1, 2) indicate that CXCR7 occurs on a wide variety of tissues and cells including monocytes, B cells, T cells and mature dendritic cells. In contrast, based on ligand binding analysis and receptor level (as assessed using anti‑CXCR7, clone 11G8), surface expression of CXCR7 was reported to be restricted to tumor cells, activated endothelial cells, fetal liver cells, and few other cell types (3). The basis of these inconsistent observations is not known but may be attributed to cell context and the use of different antibodies that may recognize different epitopes.

1. Balabanian, K. et al. (2005) J. Biol. Chem. 280:35760.
2. Infantino, S. et al. (2006) J. Immunol. 176:2197.
3. Burns, J.M. et al. (2006) J. Exp. Med. 203:2201.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ACKR3
• Uniprot:
  • AAA62370()