CXCR1/IL-8RA Antibody

Product Discontinued

CXCR1/IL-8RA Antibody Summary

• Immunogen: Synthetic peptide (selective for CXCR1receptor from near C-terminal region).
• Localization: Cell Membrane
• Specificity: This antibody detects a single 55 kDa CXCR1 in PC-CXCR1 samples. The antibody does not cross reacts with other members of the CXCR family.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: CXCR1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin 1:250
  • Immunoprecipitation >1:250
  • Western Blot >1:500
• Control:

  CXCR1/IL-8RA Overexpression Lysate

Reactivity Notes

Cross-reacts with Human and Rat. Not yet tested in other species.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Tris/Glycine buffer pH 7.5-7.9, stabilizing protein, and glycerol
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for CXCR1/IL-8RA Antibody

• CD128
• CD181 antigen
• CD181
• CDw128aC-C
• chemokine (C-X-C motif) receptor 1
• CKR-1
• CMKAR1
• C-X-C chemokine receptor type 1
• CXCR1
• CXC-R1
• CXCR-1
• High affinity interleukin-8 receptor A
• IL-8 RA
• IL-8 receptor type 1
• IL-8R A
• IL8R1
• IL8RA
• IL8RAC-C-CKR-1
• IL8RBA
• interleukin 8 receptor, alpha
• interleukin-8 receptor type 1
• interleukin-8 receptor type A

Background

Chemokine receptors belong to the superfamily of G protein coupled receptors, which regulate the trafficking and activation of leukocytes and operate as co-receptors in the entry of HIV1. CXCR1 is the receptor for interleukin-8, which is a powerful neutrophil chemotactic factor. Binding of IL-8 to CXCR1 causes activation of neutrophils. CXCR1 expression also plays an important role in many inflammatory processes.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CXCR1/IL-8RA Antibody (NB300-697)(1)

Publication using NB300-697

• Krohn N, Kapoor S, Enami Y et al. Hepatocyte transplantation-induced liver inflammation is driven by cytokines-chemokines associated with neutrophils and Kupffer cells. Gastroenterology 2009-05-01 [PMID: 19422086] (IHC-Fr, Rat)

FAQs for CXCR1/IL-8RA Antibody (NB300-697). (Showing 1 - 1 of 1 FAQ).

1. Where is the evidence that this antibody is suitable for paraffin-Immunohistochemistry?
   • Unfortunately I do not have additional data to supply for IHC-P with this antibody because we have the testing done by one of our collaborator's labs and do not have that on file. Even though I do not have additional data Novus Biologicals does have a 100% guarantee for all stated species and applications so you may receive a refund or replacement if it does not work in the applications we listed for the validated species.

Bioinformatics

• Gene Symbol: CXCR1
• Entrez:
  • 3577(Human)
  • 227288(Mouse)
  • 54258(Rat)
• Uniprot:
  • P25024(Human)