Recombinant Human CXCL17/VCC-1 Protein

SDS-Page: Human CXCL17/VCC-1 Protein [NBP2-35133]

• Reactivity: Hu
• Applications: PAGE, Bioactivity
• Format: Carrier-Free
• Concentration: LYOPH

Recombinant Human CXCL17/VCC-1 Protein Summary

• Description: A single non-glycosylated polypeptide chain containing 98 amino acids corresponding to CXCL17/VCC-1 Source: E. coli

  Uniprot ID: Q6UXB2

  Amino Acid Sequence: SSLNPGVARG HRDRGQASRR WLQEGGQECE CKDWFLRAPR RKFMTVSGLP KKQCPCDHFK GNVKKTRHQR HHRKPNKHSR ACQQFLKQCQ LRSFALPL

• Details of Functionality: CXCL17 protein is fully biologically active when compared to standard. The ED50 as determined by its ability to induce VEGF expression using murine endothelial cells is less than 5.0 ug/ml, corresponding to a specific activity of > 200 IU/mg.
• Source: E. coli
• Protein/Peptide Type: Recombinant Protein
• Gene: CXCL17
• Purity: >95%, by SDS-PAGE and HPLC
• Endotoxin Note: Less than 0.1 EU/ug of CXCL17/VCC-1 as determined by LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
  • SDS-Page
• Theoretical MW: 11.5 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles.
• Buffer: Lyophilized from a 0.2 um filtered concentrated solution in PBS, pH 7.4, with 3 % Trehalose.
• Preservative: Trehalose
• Concentration: LYOPH
• Purity: >95%, by SDS-PAGE and HPLC
• Reconstitution Instructions: Recommended to centrifuge prior to opening. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/mL.

Alternate Names for Recombinant Human CXCL17/VCC-1 Protein

• Chemokine (C-X-C Motif) Ligand 17
• C-X-C Motif Chemokine 17
• CXCL17
• Dcip1
• Dendritic Cell And Monocyte Chemokine-Like Protein
• DMC
• UNQ473
• VCC1
• VCC-1
• VCC-1/CXCL17
• VEGF Coregulated Chemokine 1
• VEGF Co-Regulated Chemokine 1

Background

CXCL17 also named dendritic cell and monocyte chemokine-like protein (DMC) and VEGF co-regulated chemokine 1 (VCC-1), is a small cytokine belonging to the CXC chemokine family. It is constitutively expressed in the lung and can be detected in trachea, stomach and skeletal muscle. CXCL17 attracts dendritic cells and monocytes and is regulated in tumors. It may be a housekeeping chemokine regulating recruitment of nonactivated blood monocytes and immature dendritic cells into tissues and may play a role in the innate defense against infections. Mature human CXCL17 shares 73 %, 71 % and 64 % amino acid sequence identity with bovine, mouse and rat CXCL17, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Publications for CXCL17/VCC-1 Protein (NBP2-35133)(1)

Publication using NBP2-35133

• Sebrell TA, Hashimi M, Sidar B et al. A Novel Gastric Spheroid Co-culture Model Reveals Chemokine-Dependent Recruitment of Human Dendritic Cells tothe Gastric Epithelium Cell Mol Gastroenterol Hepatol 2019-03-13 [PMID: 30878664] (Func, Human)

Bioinformatics

• Gene Symbol: CXCL17
• Uniprot:
  • Q6UXB2()
  • Q6UXB2()
  • Q6UXB2()