CSTF3 Antibody

Western Blot: CSTF3 Antibody [NB100-57534] - Detection of Human and Mouse CSTF77 on HeLa whole cell lysate using NB100-57534. CSTF77 was also immunoprecipitated by rabbit anti-CSTF77 antibodies NB100-57532 and NB100-57533.

Immunohistochemistry: CSTF3 Antibody [NB100-57534] - Sample: FFPE section of human breast carcinoma (left) and mouse teratoma (right). Antibody: Affinity purified rabbit anti-CSTF77 used at a dilution of 1:200 (1ug/ml). Detection: DAB

• Reactivity: Hu, Mu
• Applications: WB, IHC, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 0.2 mg/ml

CSTF3 Antibody Summary

• Immunogen: The immunogen recognized by this antibody maps to a region between residue 667 and 717 of human cleavage stimulation factor, 3' pre-RNA, subunit 3, 77kDa using the numbering given in entry NP_001317.1 (GeneID 1479).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: CSTF3
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Immunoprecipitation 2 - 5 ug/mg lysate
  • Western Blot 1:2000-1:10000
• Application Notes: Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: TBS and 0.1% BSA
• Preservative: 0.09% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for CSTF3 Antibody

• CF-1 77 kDa subunit
• Cleavage stimulation factor 77 kDa subunit
• cleavage stimulation factor subunit 3
• cleavage stimulation factor, 3' pre-RNA, subunit 3, 77kD
• cleavage stimulation factor, 3' pre-RNA, subunit 3, 77kDa
• CSTF 77 kDa subunit
• CSTF-77
• MGC117398
• MGC43001
• MGC75122

Background

The protein encoded by this gene is one of three (including CSTF1 and CSTF2) cleavage stimulation factors that combine to form the cleavage stimulation factor complex (CSTF). This complex is involved in the polyadenylation and 3' end cleavage of pre-mRNAs. The encoded protein functions as a homodimer and interacts directly with both CSTF1 and CSTF2 in the CSTF complex. Alternative splicing results in multiple transcript variants encoding different isoforms.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CSTF3
• Entrez:
  • 1479(Human)
• Uniprot:
  • NP_001317.1(Human)