HOXC11 Antibody (OTI3E10)

Western Blot: HOXC11 Antibody (3E10) [NBP2-00499] - Analysis of extracts (10ug) from 9 Human tissue by using anti-HOXC11 monoclonal antibody at 1:200 (1: Testis; 2: Omentum; 3: Uterus; 4: Breast; 5: Brain; 6: Liver; 7: Ovary; 8: Thyroid gland; 9: colon)

Immunocytochemistry/Immunofluorescence: HOXC11 Antibody (3E10) [NBP2-00499] Staining of COS7 cells transiently transfected by pCMV6-ENTRY HOXC11.

Immunohistochemistry-Paraffin: HOXC11 Antibody (3E10) [NBP2-00499] - Staining of paraffin-embedded Human tonsil using anti-HOXC11 mouse monoclonal antibody.

Flow Cytometry: HOXC11 Antibody (3E10) [NBP2-00499] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-HOXC11 antibody, and then analyzed by flow cytometry.

Western Blot: HOXC11 Antibody (3E10) [NBP2-00499] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY HOXC11 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-HOXC11.

Western Blot: HOXC11 Antibody (3E10) [NBP2-00499] - Analysis of extracts (10ug) from a mouse cell line and 3 different mouse tissues by using antiHOXC11 monoclonal antibody.(1:200)

Immunohistochemistry-Paraffin: HOXC11 Antibody (3E10) [NBP2-00499] - Staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-HOXC11 mouse monoclonal antibody.

Immunohistochemistry-Paraffin: HOXC11 Antibody (3E10) [NBP2-00499] - Staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-HOXC11 mouse monoclonal antibody.

Immunohistochemistry-Paraffin: HOXC11 Antibody (3E10) [NBP2-00499] - Staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-HOXC11 mouse monoclonal antibody.

Immunohistochemistry-Paraffin: HOXC11 Antibody (3E10) [NBP2-00499] - Staining of paraffin-embedded Human endometrium tissue using anti-HOXC11 mouse monoclonal antibody.

• Reactivity: Hu, Mu, Rt
• Applications: WB, Flow, ICC/IF, IHC
• Clone: OTI3E10
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 0.44 mg/ml

HOXC11 Antibody (OTI3E10) Summary

• Immunogen: Human recombinant protein fragment corresponding to amino acids 1-304 of human HOXC11(NP_055027) produced in E.coli.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: HOXC11
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:100
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Immunohistochemistry 1:150
  • Immunohistochemistry-Paraffin 1:150
  • Western Blot 1:500-2000
• Theoretical MW: 33.6 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 1.0% BSA and 50% Glycerol
• Preservative: 0.02% Sodium Azide
• Concentration: 0.44 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for HOXC11 Antibody (OTI3E10)

• homeo box C11
• homeobox C11
• Homeobox protein Hox-3H
• homeobox protein Hox-C11
• HOX3H
• MGC4906

Background

This gene belongs to the homeobox family of genes. The homeobox genes encode a highly conserved family of transcription factors that play an important role in morphogenesis in all multicellular organisms. Mammals possess four similar homeobox gene clusters, HOXA, HOXB, HOXC and HOXD, which are located on different chromosomes and consist of 9 to 11 genes arranged in tandem. This gene is one of several homeobox HOXC genes located in a cluster on chromosome 12. The product of this gene binds to a promoter element of the lactase-phlorizin hydrolase. It also may play a role in early intestinal development. An alternatively spliced variant encoding a shorter isoform has been described but its full-length nature has not been determined. [provided by RefSeq]. COMPLETENESS: complete on the 3' end.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for HOXC11 Antibody (NBP2-00499)(2)

Publications using NBP2-00499

• Peng X, Liu X, Hu W et al. HOXC11 drives lung adenocarcinoma progression through transcriptional regulation of SPHK1 Cell death & disease 2023-02-23 [PMID: 36823149] (Western Blot, Immunocytochemistry/ Immunofluorescence, Human)
• Peng X, Sun J, Long Y et al. The Significance of HOXC11 and LSH in Survival Prediction in Gastric Adenocarcinoma OncoTargets and therapy 2021-03-01 [PMID: 33688200] (IF/IHC, WB, Human)

FAQs for HOXC11 Antibody (NBP2-00499). (Showing 1 - 1 of 1 FAQs).

1. I have been using your HoxC11 3E10 primary for IHC staining and it interested to the epitope that is targeted by the Ab and also your IHC protocol you used to produce the images in particular the Antigen retrieval method.
   • The immunogen used to generate our HoxC11 antibody with catalogue number NBP2-00499 was a human recombinant protein fragment corresponding to amino acids 1-304 of human HOXC11 (NP_055027) produced in E.coli. We do not epitope map our antibodies and so, unfortunately, I am unable to provide you with any further information regarding the binding of this antibody to its target. Here is a link to our standard IHC-P protocol. Here is the antigen retrieval method, which uses sodium citrate buffer. Antigen Retrieval Solution: 0.01M Sodium Citrate Buffer, pH 6.0, To prepare stock solutions: Solution A. 0.1 M citric acid solution: dissolve 21.0 g of citric acid, monohydrate (C6H8O7.H2O) in 100 ml of dH2O. Solution B. 0.1M sodium citrate solution: dissolve 29.4 g trisodium citrate dihydrate (C6H5Na3O7.2H2O) in 100 ml of dH2O. Working solution: Add 9 ml of Stock solution A and 41 ml of stock solution B to 450 ml of dH2O. Adjust pH to 6.0. Antigen Retrieval: Pre-heat steamer or water bath with staining dish containing Sodium Citrate Buffer until temperature reaches 95-100 degrees Celsius. Immerse slides in the staining dish. Place the lid loosely on the staining dish and incubate for 20-40 minutes. Remove the staining dish to room temperature and allow the slides to cool for 20 minutes before proceeding with normal staining procedure.

Bioinformatics

• Gene Symbol: HOXC11
• Entrez:
  • 3227(Human)