CSN1 Antibody - BSA Free

Western Blot: CSN1 Antibody [NB100-362] - Detection of Human CSN1 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa and 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-CSN1 antibody NB100-362 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

Immunohistochemistry: CSN1 Antibody [NB100-362] - Sample: FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit anti-CSN1 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB

Western Blot: CSN1 Antibody [NB100-362] - Whole cell lysate (50 ug - E; 10 ug - T) from mock transfected (E) or CSN1 transfected (T) HEK293T cells. Antibody used at the indicated concentration.

Western Blot: CSN1 Antibody [NB100-362] - Samples : Whole cell lysate (50 ug - E; 10 ug - T) from mock transfected (E) or CSN1 transfected (T) HEK293T cells. Antibody : Affinity purified rabbit anti-CSN1 used at the indicated concentration. Detection : Chemiluminescence with a 10 second exposure.

Immunohistochemistry-Paraffin: CSN1 Antibody [NB100-362] - FFPE section of human prostate-nodular hypertrophy. Affinity purified rabbit anti-CSN1 used at a dilution of 1:250. Detection: DAB staining using Immunohistochemistry Accessory Kit

• Reactivity: Hu
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

CSN1 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit CSN1 Antibody - BSA Free (NB100-362) is a polyclonal antibody validated for use in IHC and WB. Anti-CSN1 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: The immunogen recognized by this antibody maps to a region between residue 400 and the C-terminus (residue 471) of human G protein Pathway Suppressor 1 using the numbering given in Swiss-Prot entry Q13098 (GeneID 2873).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: GPS1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:500- 1:2000
  • Western Blot 1:1000-1:10000
• Application Notes: Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Tris-Citrate/Phosphate (pH 7.0 - 8.0)
• Preservative: 0.09% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for CSN1 Antibody - BSA Free

• COP9 signalosome complex subunit 1
• COPS1JAB1-containing signalosome subunit 1
• CSN1MGC71287
• G protein pathway suppressor 1SGN1
• GPS-1
• Protein MFH
• Signalosome subunit 1

Background

COP9 signalosome is a conserved protein composed of eight subunits. Individual subunits of the complex have been linked to various signal transduction pathways leading to gene expression and cell cycle control. CSN1 is the COP9 subunit 1. The C-terminus of CSN1 is responsible for interacting with CSN2, CSN3, and CSN4 subunits. It is required for incorporation of the subunit into the complex. The N-terminus harbors the activity domain that confers most of the repression functions of CSN1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CSN1 Antibody (NB100-362)(1)

Publication using NB100-362

• Olma MH, Roy M, Le Bihan T et al. An interaction network of the mammalian COP9 signalosome identifies Dda1 as a core subunit of multiple Cul4-based E3 ligases. J Cell Sci 2009-04-01 [PMID: 19295130]

Bioinformatics

• Gene Symbol: GPS1
• Entrez:
  • 2873(Human)
• Uniprot:
  • Q13098(Human)