CSH2 Overexpression Lysate (Native)

Images

 
Western Blot: CSH2 Overexpression Lysate (Adult Normal) [NBL1-09523] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for CSH2.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

CSH2 Overexpression Lysate (Native) Summary

Immunogen
The lysate was created in HEK293T cells, using plasmid ID RC219345 and based on accession number NM_022644. The protein contains a C-terminal DDK tag.
Specificity
Homo sapiens chorionic somatomammotropin hormone 2 (CSH2), transcript variant 2, mRNA.
Gene
CSH2

Applications/Dilutions

Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Theoretical MW
18.6 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for CSH2

Type
Overexpression
Protein State
Native

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for CSH2 Overexpression Lysate (Native)

  • chorionic somatomammotropin B
  • chorionic somatomammotropin hormone 2
  • CS-2
  • CSBChoriomammotropin
  • hCS-B
  • Lactogen
  • PL
  • Placental lactogen

Background

The protein encoded by this gene is a member of the somatotropin/prolactin family of hormones and plays an important role in growth control. The gene is located at the growth hormone locus on chromosome 17 along with four other related genes in the same transcriptional orientation; an arrangement which is thought to have evolved by a series of gene duplications. Although the five genes share a remarkably high degree of sequence identity, they are expressed selectively in different tissues. Alternative splicing generates additional isoforms of each of the five growth hormones. This particular family member is expressed mainly in the placenta and utilizes multiple transcription initiation sites. Expression of the identical mature proteins for chorionic somatomammotropin hormones 1 and 2 is upregulated during development, while the ratio of 1 to 2 increases by term. Structural and expression differences provide avenues for developmental regulation and tissue specificity. [provided by RefSeq]. Transcript Variant: This variant (2) contains intron D and encodes an isoform (2) that diverges from all other CS isoforms in the carboxy-terminus due to a frameshift. Early truncation occurs relative to a similarly spliced variant of chorionic somatomammotropin hormone 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Customers Who Viewed This Item Also Viewed...

AF682
Species: Hu
Applications: WB, IHC, ELISA(Cap), ELISA(Det), Neut, ELISA(Sta)
AF1067
Species: Hu, Mu, Rt
Applications: WB, IHC, Neut
NBP2-46376
Species: Hu
Applications: WB, IHC
AF1067
Species: Hu, Mu, Rt
Applications: WB, IHC, Neut
AF1152
Species: Hu
Applications: WB, Simple Western, IHC, CyTOF-ready, ICFlow
NBP2-34260
Species: Hu, Mu, Po, Bv, Rb
Applications: WB, Flow, ICC/IF, IHC-Fr, IHC-P, IP, PAGE
H00005087-M01
Species: Hu
Applications: WB, ELISA, ICC/IF, IHC-P
NB100-61070
Species: Hu
Applications: WB (-), IP
NBP1-32789
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
NBP2-45519
Species: Hu
Applications: WB, IHC
MAB1368
Species: Hu, Mu
Applications: WB, Simple Western, CyTOF-ready, ICC, ICFlow, KO
MAB4169
Species: Hu
Applications: WB, IHC
H00093659-T01
Species: Hu
NBP1-47801
Species: Hu, Mu, Rt, Ca, Mk, Pm
Applications: WB, Flow, ICC/IF, IHC, IHC-P
NB600-233
Species: Hu, Mu
Applications: WB, IHC, IHC-P, IP, PLA
MAB6799
Species: Hu
Applications: WB, IHC
NBP2-01223
Species: Hu
Applications: WB, Flow, IHC, IHC-P

Publications for CSH2 Lysate (NBL1-09523) (0)

There are no publications for CSH2 Lysate (NBL1-09523).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for CSH2 Lysate (NBL1-09523) (0)

There are no reviews for CSH2 Lysate (NBL1-09523). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen
  • 1st to review with an image -- $50/€35/£30/$50 CAD/¥300 Yuan/¥5000 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for CSH2 Lysate (NBL1-09523) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Additional CSH2 Products

Bioinformatics Tool for CSH2 Lysate (NBL1-09523)

Discover related pathways, diseases and genes to CSH2 Lysate (NBL1-09523). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for CSH2 Lysate (NBL1-09523)

Discover more about diseases related to CSH2 Lysate (NBL1-09523).
 

Pathways for CSH2 Lysate (NBL1-09523)

View related products by pathway.

PTMs for CSH2 Lysate (NBL1-09523)

Learn more about PTMs related to CSH2 Lysate (NBL1-09523).

Blogs on CSH2

There are no specific blogs for CSH2, but you can read our latest blog posts.

Customers Who Bought This Also Bought

CSH2 Antibody
NBP2-16019

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our CSH2 Overexpression Lysate (Native) and receive a gift card or discount.

Bioinformatics

Gene Symbol CSH2