CROCC Antibody - BSA Free

Immunohistochemistry-Paraffin: CROCC Antibody [NBP1-80820] - Staining in human fallopian tube and liver tissues using NBP1-80820 antibody. Corresponding CROCC RNA-seq data are presented for the same tissues.

Immunohistochemistry-Paraffin: CROCC Antibody [NBP1-80820] - Staining of human bronchus, fallopian tube, liver and testis using Anti-CROCC antibody NBP1-80820 (A) shows similar protein distribution across tissues to independent antibody NBP1-80821 (B).

Western Blot: CROCC Antibody [NBP1-80820] - Analysis in human cell line SCLC-21H.

Immunocytochemistry/Immunofluorescence: CROCC Antibody [NBP1-80820] - Staining of human cell line U-2 OS shows localization to plasma membrane, centrosome & actin filaments. Antibody staining is shown in green.

Immunohistochemistry-Paraffin: CROCC Antibody [NBP1-80820] - Staining of human bronchus shows strong positivity in apical membrane in respiratory epithelial cells.

Immunohistochemistry-Paraffin: CROCC Antibody [NBP1-80820] - Staining of human Fallopian tube shows strong membranous positivity in glandular cells.

Immunohistochemistry-Paraffin: CROCC Antibody [NBP1-80820] - Staining of human liver shows no positivity in hepatocytes as expected.

Immunohistochemistry-Paraffin: CROCC Antibody [NBP1-80820] - Staining of human testis shows very weak cytoplasmic positivity in Leydig cells.

• Reactivity: Hu
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

CROCC Antibody - BSA Free Summary

• Immunogen: This antibody was developed against Recombinant Protein corresponding to amino acids: VKRLEKQNLEKDQVNKDLTEKLEALESLRLQEQAALETEDGEGLQQTLRDLAQAVLSDSESGVQLSGSERTADASNGS
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: CROCC
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:200 - 1:500
  • Immunohistochemistry-Paraffin 1:200 - 1:500
  • Western Blot 0.04-0.4 ug/ml
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF, Fixation Permeabilization: Use PFA/Triton X-100.
• Control Peptide:

  CROCC Protein (NBP1-80820PEP)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for CROCC Antibody - BSA Free

• CROCC ciliary rootlet coiled-coil, rootletin
• CROCC
• CROCC1
• KIAA0445
• ROLT
• TAX1BP2

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CROCC Antibody (NBP1-80820)(13)

Publications using NBP1-80820

• Murakami S, Soyama A, Miyamoto D et al. Transplantation of chemically-induced liver progenitor cells ameliorates hepatic fibrosis in mice with diet-induced nonalcoholic steatohepatitis Regenerative Therapy 2022-12-01 [PMID: 36475025] (IF/IHC, ICC/IF, Rat)
• Mahen R. cNap1 bridges centriole contact sites to maintain centrosome cohesion PLOS Biology 2022-10-25 [PMID: 36282799]
• Faubel RJ, Santos Canellas VS, Gaesser J et al. Flow blockage disrupts cilia-driven fluid transport in the epileptic brain Acta neuropathologica 2022-08-18 [PMID: 35980457]
• Chu Z, Gruss OJ Mitotic Maturation Compensates for Premature Centrosome Splitting and PCM Loss in Human cep135 Knockout Cells Cells 2022-04-01 [PMID: 35406752] (ICC/IF, Human)
• Verschueren A, Boucherit L, Ferrari U et al. Planar polarity in primate cone photoreceptors: a potential role in Stiles Crawford effect phototropism Communications Biology 2022-01-24 [PMID: 35075261]
• Park HS, Pich C, Chiticariu E, Bachmann D ARP-T1 is a ciliary protein associated with a novel ciliopathy in inherited basal cell cancer, Bazex-DuprE-Christol Syndrome bioRxiv 2019-12-16 [PMID: 33972689] (ICC/IF, Human)
• Park HS, Papanastasi E, Blanchard G et al. ARP-T1-associated Bazex-DuprE-Christol syndrome is an inherited basal cell cancer with ciliary defects characteristic of ciliopathies Communications biology 2021-05-10 [PMID: 33972689] (IF/IHC, Human)
• Xu G, Wei X, Tu Q, Zhou C. Up-regulated microRNA-33b inhibits epithelial-mesenchymal transition in gallbladder cancer through down-regulating CROCC Bioscience Reports 2020-01-31 [PMID: 31799620]
• Mahen R. Stable centrosomal roots disentangle to allow interphase centriole independence. bioRxiv. [PMID: 29649211] (ICC/IF, Human)
• Flanagan AM, Stavenschi E, Basavaraju S et al. Centriole splitting caused by loss of the centrosomal linker protein C-NAP1 reduces centriolar satellite density and impedes centrosome amplification. Mol Biol Cell 2017-03-15 [PMID: 28100636] (WB)
• Wallmeier J, Al-Mutairi DA, Chen CT et al. Mutations in CCNO result in congenital mucociliary clearance disorder with reduced generation of multiple motile cilia. Nat Genet 2014-06-01 [PMID: 24747639]
• Rohena CC, Peng J, Johnson TA et al. Chemically Diverse Microtubule Stabilizing Agents Initiate Distinct Mitotic Defects and Dysregulated Expression of Key Mitotic Kinases. Biochem Pharmacol 2013-02-08 [PMID: 23399639] (ICC/IF, Human)
• Loffler H, Fechter A, Liu FY et al. DNA damage-induced centrosome amplification occurs via excessive formation of centriolar satellites. Oncogene. 2012-07-23 [PMID: 22824794]

Bioinformatics

• Gene Symbol: CROCC
• Entrez:
  • 9696(Human)