CROCC Antibody (2734A) [Unconjugated]

Western blot shows lysates of MDA‑MB‑231 human breast cancer cells and MDA-MB-231 + Calyculin A. PVDF membrane was probed with 1 µg/mL of Rabbit Anti-Human CROCC Monoclonal Antibody (Catalog # MAB11335) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for CROCC at approximately ~228 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.

CROCC was detected in immersion fixed HEL 92.1.7 Human Erythroleukemic Cells and Daudi Human Burkitt's Lymphoma Cells (Both Positive) using Rabbit Anti-Human CROCC Monoclonal Antibody (Catalog # MAB11335) at 3 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rabbit IgG Secondary Antibody (red; Catalog # NL004) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

• Reactivity: Hu
• Applications: WB, ICC/IF
• Clone: 2734A
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated

CROCC Antibody (2734A) [Unconjugated] Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Phosphopeptide containing the human CROCC S site 1476
  Accession # Q5TZA2
• Specificity: Detects Human Phospho-CROCC in direct ELISA
• Source: N/A
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Purity Statement: Protein A or G purified from cell culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry 3-15 ug/mL
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CROCC Antibody (2734A) [Unconjugated]

• CROCC ciliary rootlet coiled-coil, rootletin
• CROCC
• CROCC1
• KIAA0445
• ROLT
• TAX1BP2

Background

Human CROCC (also known as Rootletin) is a large coiled-coil protein that is primarily expressed in ciliated cells and plays a critical role in the organization and maintenance of the ciliary structure. Cilia are hair-like structures that protrude from the surface of many eukaryotic cells and are involved in a variety of cellular functions, including motility and signaling.CROCC is essential for forming and maintaining the rootlet and has been shown to interact with other centrosomal and ciliary proteins, such as PCM-1, C-Nap1, and CEP68. CROCC has also been shown to interact with microtubules, suggesting that it may play a role in regulating microtubule dynamics. CROCC can be Phosphorylated by NEK2 which may regulate its association with centrosomes. This antibody detects CROCC phosphorylated at Ser1476. Two alternatively spliced isoforms have been reported.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Uniprot:
  • Q5TZA2()