CRLR Antibody - BSA Free

Immunohistochemistry-Paraffin: CRLR Antibody [NLS6731] - Vessel, endothelium

Immunohistochemistry-Paraffin: CRLR Antibody [NLS6731] - Analysis of anti-CALCRL / CGRP Receptor antibody with colon, submucosal plexus and submucosal vessels at 34 ug/ ml.

Immunohistochemistry-Paraffin: CRLR Antibody [NLS6731] - Analysis of anti-CALCRL / CGRP Receptor antibody with human bone, osteosarcoma.

Immunohistochemistry-Paraffin: CRLR Antibody [NLS6731] - Anti-CALCRL / CGRP Receptor antibody IHC of human Lung, Non-Small Cell Carcinoma. Tissue after heat-induced antigen retrieval.

Immunohistochemistry-Paraffin: CRLR Antibody [NLS6731] - Breast, adenocarcinoma

Immunohistochemistry-Paraffin: CRLR Antibody [NLS6731] - Brain Amygdala Neurons

Immunohistochemistry-Paraffin: CRLR Antibody - BSA Free [NLS6731] - Immunohistochemistry of formalin-fixed, paraffin-embedded Human Brain, Cortex tissue

• Reactivity: Hu, Rt, Po, Bv, Ca, Eq, Gt, Ha, Pm, Pm, Rb, Xp, Mu, Bt, Fi, Gp, Ma-Op, Pm-Or
• Applications: IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

CRLR Antibody - BSA Free Summary

• Description: Product can be stored undiluted at 4C for up to 1 month.
• Immunogen: Synthetic 16 amino acid peptide from 3rd cytoplasmic domain of human CRLR.
• Epitope: 3rd cytoplasmic domain
• Specificity: Human CALCRL. BLAST analysis of the peptide immunogen showed no homology with other human proteins, except CALCR (56%).
• Predicted Species: Mouse (94%), Opossum (100%), Fish (94%), Guinea Pig (94%), Orangutan (100%), Bat (94%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: CALCRL
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 10-20 ug/ml
• Application Notes: .

Reactivity Notes

Predicted cross-reactivity based on sequence identity: Gorilla (100%), Gibbon (100%), Marmoset (100%), Turkey (81%), Chicken (81%).

Packaging, Storage & Formulations

• Storage: Keep as concentrated solution. Aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.1% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for CRLR Antibody - BSA Free

• calcitonin gene-related peptide type 1 receptor
• calcitonin receptor-like
• CALCRL
• CGRP type 1 receptor
• CGRPR
• CGRPRCRLRCalcitonin receptor-like receptor
• CRLR

Background

Calcitonin receptor-like receptor (CRLR) is an Adrenomedullin Receptor that requires two additional proteins to function: a chaperone protein RAMP (receptor activity modifying protein) and the component protein RCP. The function of CRLR depends on its interaction with RAMP. When coexpressed with RAMP1, CRLR acts as a receptor for calcitonin gene-related peptide (CGRP). In contrast, when CRLR is coexpressed with either RAMP2 or RAMP3, it acts as a receptor for adrenomedullin. Activation of the receptor leads to an increase in intracellular cyclic AMP. CRLR mediates the vasorelaxation of arteries, suggesting a potential role for the receptor in reaction to injury, particularly in the treatment of pulmonary hypertension. CRLR expression has been reported in brain, lung, blood vessel, liver, and intestinal tract. ESTs have been isolated from B-Cell/lung/testis, bone marrow, embryo, lung, and synovium libraries.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CRLR Antibody (NLS6731)(1)

Publication using NLS6731

• De Logu F, Nassini R, Hegron A Et al. CGRP Signals from Endosomes of Schwann Cells to Elicit Migraine Pain Nat Commun 2022-02-04 [PMID: 35115501]

FAQs for CRLR Antibody (NLS6731). (Showing 1 - 1 of 1 FAQ).

1. Hello, I am interested in NLS6731, and would like to use it in rat. Please can you let me know the amino acids it was raised against in the human sequence? Thank-you, Kim
   • The exact sequence is proprietary, but the immunogen is a peptide from the 3rd cytoplasmic loop of CRLR, which consists of aa314-336 and is 100% identical in rat.

Bioinformatics

• Gene Symbol: CALCRL
• Entrez:
  • 10203(Human)