CrkL Antibody

Western Blot: CrkL Antibody [H00001399-D01P] - Analysis of CRKL expression in A-431.

Immunocytochemistry/Immunofluorescence: CrkL Antibody [H00001399-D01P] - Analysis of antibody to CRKL on HeLa cell. Antibody concentration 10 ug/ml.

Western Blot: CrkL Antibody [H00001399-D01P] - Analysis of CRKL expression in transfected 293T cell line by CRKL polyclonal antibody.Lane 1: CRKL transfected lysate(33.80 KDa).Lane 2: Non-transfected lysate.

Proximity Ligation Assay: CrkL Antibody [H00001399-D01P] - Analysis of protein-protein interactions between CRKL and EGFR. HeLa cells were stained with anti-CRKL rabbit purified polyclonal 1:1200 and anti-EGFR mouse purified polyclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).

Proximity Ligation Assay: CrkL Antibody [H00001399-D01P] - Analysis of protein-protein interactions between CRKL and SOS1. Huh7 cells were stained with anti-CRKL rabbit purified polyclonal 1:1200 and anti-SOS1 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).

Proximity Ligation Assay: CrkL Antibody [H00001399-D01P] - Analysis of protein-protein interactions between CRKL and PTPN11. Mahlavu cells were stained with anti-CRKL rabbit purified polyclonal 1:600 and anti-PTPN11 mouse purified polyclonal antibody 1:100. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).

Product Discontinued

CrkL Antibody Summary

• Description: Quality control test: Antibody reactive against mammalian transfected lysate.
• Immunogen: CRKL (NP_005198.1, 1 a.a. - 303 a.a.) full-length human protein. MSSARFDSSDRSAWYMGPVSRQEAQTRLQGQRHGMFLVRDSSTCPGDYVLSVSENSRVSHYIINSLPNRRFKIGDQEFDHLPALLEFYKIHYLDTTTLIEPAPRYPSPPMGSVSAPNLPTAEDNLEYVRTLYDFPGNDAEDLPFKKGEILVIIEKPEEQWWSARNKDGRVGMIPVPYVEKLVRSSPHGKHGNRNSNSYGIPEPAHAYAQPQTTTPLPAVSGSPGAAITPLPSTQNGPVFAKAIQKRVPCAYDKTALALEVGDIVKVTRMNINGQWEGEVNGRKGLFPFTHVKIFDPQNPDENE
• Specificity: CRKL - v-crk sarcoma virus CT10 oncogene homolog (avian)-like,
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: CRKL
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence
  • Proximity Ligation Assay
  • Western Blot
• Application Notes: Antibody reactivity against Recombinant Protein with GST tag on ELISA and WB and also on transfected lysate in WB. GST tag alone is used as a negative control.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.4)
• Preservative: No Preservative
• Purity: Protein A purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for CrkL Antibody

• CrkL
• crk-like protein
• pCRKL
• v-crk avian sarcoma virus CT10 oncogene homolog-like
• v-crk sarcoma virus CT10 oncogene homolog (avian)-like

Background

This gene encodes a protein kinase containing SH2 and SH3 (src homology) domains which has been shown to activate the RAS and JUN kinase signaling pathways and transform fibroblasts in a RAS-dependent fashion. It is a substrate of the BCR-ABL tyrosine kinase, plays a role in fibroblast transformation by BCR-ABL, and may be oncogenic.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CrkL Antibody (H00001399-D01P)(1)

Publication using H00001399-D01P

• Liu CH, Chen TC, Chau GY et al. An analysis of protein-protein interactions in cross-talk pathways reveals CRKL as a novel prognostic marker in hepatocellular carcinoma. Mol Cell Proteomics 2013-02-08 [PMID: 23397142]

Bioinformatics

• Gene Symbol: CRKL
• Entrez:
  • 1399(Human)
• Uniprot:
  • NP_005198.1(Human)