Crk Overexpression Lysate (Native)

Images

 
Western Blot: Crk Overexpression Lysate (Adult Normal) [NBL1-09484] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Crk.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

Crk Overexpression Lysate (Native) Summary

Immunogen
The lysate was created in HEK293T cells, using plasmid ID RC203438 and based on accession number NM_005206. The protein contains a C-terminal DDK tag.
Specificity
Homo sapiens v-crk sarcoma virus CT10 oncogene homolog (avian) (CRK), transcript variant I, mRNA.
Gene
CRK

Applications/Dilutions

Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Theoretical MW
22.7 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for Crk

Type
Overexpression
Protein State
Native

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for Crk Overexpression Lysate (Native)

  • adapter molecule crk
  • avian sarcoma virus CT10 (v-crk) oncogene homolog
  • Crk
  • CRKII
  • p38
  • Proto-oncogene c-Crk
  • v-crk avian sarcoma virus CT10 oncogene homolog
  • v-crk sarcoma virus CT10 oncogene homolog (avian)

Background

This gene encodes a member of an adapter protein family that binds to several tyrosine-phosphorylated proteins. The product of this gene has several SH2 and SH3 domains (src-homology domains) and is involved in several signaling pathways, recruiting cytoplasmic proteins in the vicinity of tyrosine kinase through SH2-phosphotyrosine interaction. The N-terminal SH2 domain of this protein functions as a positive regulator of transformation whereas the C-terminal SH3 domain functions as a negative regulator of transformation. Two alternative transcripts encoding different isoforms with distinct biological activity have been described.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Publications for Crk Lysate (NBL1-09484) (0)

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Bioinformatics

Gene Symbol CRK
Entrez