Crk Antibody

Images

 
Western Blot: Crk Antibody [H00001398-D01P] - Analysis of CRK expression in transfected 293T cell line by CRK polyclonal antibody.Lane 1: CRK transfected lysate(22.90 KDa).Lane 2: Non-transfected lysate.
Immunocytochemistry/ Immunofluorescence: Crk Antibody [H00001398-D01P] - Analysis of antibody to CRK on HeLa cell. Antibody concentration 10 ug/ml.
Proximity Ligation Assay: Crk Antibody [H00001398-D01P] - Analysis of protein-protein interactions between CRK and SOS1. HeLa cells were stained with anti-CRK rabbit purified polyclonal 1:1200 and anti-SOS1 mouse ...read more
Proximity Ligation Assay: Crk Antibody [H00001398-D01P] - Analysis of protein-protein interactions between CRK and SHC1. Mahlavu cells were stained with anti-CRK rabbit purified polyclonal 1:1200 and anti-SHC1 mouse ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF, PLA
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

Crk Antibody Summary

Immunogen
CRK (NP_005197.3, 1 a.a. - 204 a.a.) full-length human protein. MAGNFDSEERSSWYWGRLSRQEAVALLQGQRHGVFLVRDSSTSPGDYVLSVSENSRVSHYIINSSGPRPPVPPSPAQPPPGVSPSRLRIGDQEFDSLPALLEFYKIHYLDTTTLIEPVSRSRQGSGVILRQEEAEYVRALFDFNGNDEEDLPFKKGDILRIRDKPEEQWWNAEDSEGKRGMIPVPYVEKYRPASASVSALIGGR
Specificity
Reacts with v-crk sarcoma virus CT10 oncogene homolog (avian).
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
CRK
Purity
Protein A purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Western Blot
  • Immunocytochemistry/Immunofluorescence
  • Proximity Ligation Assay
Application Notes
This antibody is reactive against transfected lysate in WB and as a detection antibody in ELISA.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.4)
Preservative
No Preservative
Purity
Protein A purified

Notes

Quality control test: Antibody reactive against mammalian transfected lysate.

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for Crk Antibody

  • adapter molecule crk
  • avian sarcoma virus CT10 (v-crk) oncogene homolog
  • Crk
  • CRKII
  • p38
  • Proto-oncogene c-Crk
  • v-crk avian sarcoma virus CT10 oncogene homolog
  • v-crk sarcoma virus CT10 oncogene homolog (avian)

Background

This gene encodes a member of an adapter protein family that binds to several tyrosine-phosphorylated proteins. The product of this gene has several SH2 and SH3 domains (src-homology domains) and is involved in several signaling pathways, recruiting cytoplasmic proteins in the vicinity of tyrosine kinase through SH2-phosphotyrosine interaction. The N-terminal SH2 domain of this protein functions as a positive regulator of transformation whereas the C-terminal SH3 domain functions as a negative regulator of transformation. Two alternative transcripts encoding different isoforms with distinct biological activity have been described. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol CRK
Entrez
Uniprot