Crk Antibody

Western Blot: Crk Antibody [H00001398-D01P] - Analysis of CRK expression in transfected 293T cell line by CRK polyclonal antibody.Lane 1: CRK transfected lysate(22.90 KDa).Lane 2: Non-transfected lysate.

Immunocytochemistry/Immunofluorescence: Crk Antibody [H00001398-D01P] - Analysis of antibody to CRK on HeLa cell. Antibody concentration 10 ug/ml.

Proximity Ligation Assay: Crk Antibody [H00001398-D01P] - Analysis of protein-protein interactions between CRK and SOS1. HeLa cells were stained with anti-CRK rabbit purified polyclonal 1:1200 and anti-SOS1 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).

Proximity Ligation Assay: Crk Antibody [H00001398-D01P] - Analysis of protein-protein interactions between CRK and SHC1. Mahlavu cells were stained with anti-CRK rabbit purified polyclonal 1:1200 and anti-SHC1 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).

Product Discontinued

Crk Antibody Summary

• Description: Quality control test: Antibody reactive against mammalian transfected lysate.
• Immunogen: CRK (NP_005197.3, 1 a.a. - 204 a.a.) full-length human protein. MAGNFDSEERSSWYWGRLSRQEAVALLQGQRHGVFLVRDSSTSPGDYVLSVSENSRVSHYIINSSGPRPPVPPSPAQPPPGVSPSRLRIGDQEFDSLPALLEFYKIHYLDTTTLIEPVSRSRQGSGVILRQEEAEYVRALFDFNGNDEEDLPFKKGDILRIRDKPEEQWWNAEDSEGKRGMIPVPYVEKYRPASASVSALIGGR
• Specificity: Reacts with v-crk sarcoma virus CT10 oncogene homolog (avian).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: CRK
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence
  • Proximity Ligation Assay
  • Western Blot
• Application Notes: This antibody is reactive against transfected lysate in WB and as a detection antibody in ELISA.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.4)
• Preservative: No Preservative
• Purity: Protein A purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for Crk Antibody

• adapter molecule crk
• avian sarcoma virus CT10 (v-crk) oncogene homolog
• Crk
• CRKII
• p38
• Proto-oncogene c-Crk
• v-crk avian sarcoma virus CT10 oncogene homolog
• v-crk sarcoma virus CT10 oncogene homolog (avian)

Background

This gene encodes a member of an adapter protein family that binds to several tyrosine-phosphorylated proteins. The product of this gene has several SH2 and SH3 domains (src-homology domains) and is involved in several signaling pathways, recruiting cytoplasmic proteins in the vicinity of tyrosine kinase through SH2-phosphotyrosine interaction. The N-terminal SH2 domain of this protein functions as a positive regulator of transformation whereas the C-terminal SH3 domain functions as a negative regulator of transformation. Two alternative transcripts encoding different isoforms with distinct biological activity have been described. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CRK
• Entrez:
  • 1398(Human)
• Uniprot:
  • NP_005197.3(Human)