Crk Antibody

Western Blot: Crk Antibody [H00001398-B01P] - Analysis of CRK expression in transfected 293T cell line by CRK polyclonal antibody. Lane 1: CRK transfected lysate(22.90 KDa). Lane 2: Non-transfected lysate.

Proximity Ligation Assay: Crk Antibody [H00001398-B01P] - Analysis of protein-protein interactions between FLT1 and CRK. Huh7 cells were stained with anti-FLT1 rabbit purified polyclonal 1:600 and anti-CRK mouse purified polyclonal antibody 1:100. Each red dot represents the detection of protein-protein interact

Product Discontinued

Crk Antibody Summary

• Description: Quality control test: Antibody reactive against mammalian transfected lysate.
• Immunogen: CRK (NP_005197.3, 1 a.a. - 204 a.a.) full-length human protein. MAGNFDSEERSSWYWGRLSRQEAVALLQGQRHGVFLVRDSSTSPGDYVLSVSENSRVSHYIINSSGPRPPVPPSPAQPPPGVSPSRLRIGDQEFDSLPALLEFYKIHYLDTTTLIEPVSRSRQGSGVILRQEEAEYVRALFDFNGNDEEDLPFKKGDILRIRDKPEEQWWNAEDSEGKRGMIPVPYVEKYRPASASVSALIGGR
• Isotype: IgG
• Clonality: Polyclonal
• Host: Mouse
• Gene: CRK
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Proximity Ligation Assay
  • Western Blot
• Application Notes: It has been used for WB.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.4)
• Preservative: No Preservative
• Purity: Protein G purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for Crk Antibody

• adapter molecule crk
• avian sarcoma virus CT10 (v-crk) oncogene homolog
• Crk
• CRKII
• p38
• Proto-oncogene c-Crk
• v-crk avian sarcoma virus CT10 oncogene homolog
• v-crk sarcoma virus CT10 oncogene homolog (avian)

Background

This gene encodes a member of an adapter protein family that binds to several tyrosine-phosphorylated proteins. The product of this gene has several SH2 and SH3 domains (src-homology domains) and is involved in several signaling pathways, recruiting cytoplasmic proteins in the vicinity of tyrosine kinase through SH2-phosphotyrosine interaction. The N-terminal SH2 domain of this protein functions as a positive regulator of transformation whereas the C-terminal SH3 domain functions as a negative regulator of transformation. Two alternative transcripts encoding different isoforms with distinct biological activity have been described.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Crk Antibody (H00001398-B01P)(1)

Publication using H00001398-B01P

• Liu CH, Chen TC, Chau GY et al. An analysis of protein-protein interactions in cross-talk pathways reveals CRKL as a novel prognostic marker in hepatocellular carcinoma. Mol Cell Proteomics 2013-02-08 [PMID: 23397142]

Bioinformatics

• Gene Symbol: CRK
• Uniprot:
  • NP_005197.3(Human)