CRISP-3 Antibody (295208) [Alexa Fluor® 700]

Images

 
There are currently no images for CRISP-3 Antibody (IC23972N).

Every product we sell is backed by Novus' 100% Guarantee. If you have used this product, please submit your images and reviews to earn reward points.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Flow
Clone
295208
Clonality
Monoclonal
Host
Mouse
Conjugate
Alexa Fluor 700
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.

CRISP-3 Antibody (295208) [Alexa Fluor® 700] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant human CRISP-3
Asn21-Tyr245 (Ser106Pro & Ala134Ser)
Accession # P54108.1
Specificity
Detects human CRISP-3 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human CRISP-2 is observed.
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
CRISP3
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Intracellular Staining by Flow Cytometry 0.25-1 ug/10^6 cells
Application Notes
Flow Cytometry: Please use 0.25-1 ug of conjugated antibody per 10e6 cells.

Packaging, Storage & Formulations

Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Buffer
Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.
Preservative
0.09% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CRISP-3 Antibody (295208) [Alexa Fluor® 700]

  • Aeg2
  • CRISP3
  • CRISP-3
  • CRISP-3MGC126588
  • CRS3
  • cysteine-rich secretory protein 3
  • cysteine-rich secretory protein-3
  • dJ442L6.3
  • SGP28
  • SGP28Aeg2
  • specific granule protein (28 kDa)
  • Specific granule protein of 28 kDa

Background

CRISP-3 is one of three CRISPs (cysteine-rich secretory proteins) found in mammalian exocrine secretions and granulocytes that may play a role in innate immunity (1‑3). CRISPs and several snake, insect, and lizard venom proteins are characterized by 16 invariant cysteine residues (4). Structurally, they consist of an N-terminal SCP domain, a hinge region, and a cysteine-rich domain (5). CRISP-3 is produced by salivary, pancreas, prostate, and lacrimal glands, as well as spermatozoa and mature spermatids (2, 6, 7). In mouse, however, CRISP-3 has not been detected in the male genital tract (8, 9). CRISP-3 is up-regulated in epithelial prostate cancer and chronic pancreatitis (10, 11). It is present as 30 kDa and 28 kDa species, corresponding to glycosylated and nonglycosylated forms (1, 3, 7, 10, 12). In serum and seminal fluid, CRISP-3 forms high affinity noncovalent complexes with the more abundant alpha 1B-glycoprotein and beta -microseminoprotein/PSP94, respectively (12, 13). Binding is mediated by the SCP domain of CRISP-3 and is independent of glycosylation (12). CRISP-3 is also expressed in pre-B cells but not in T cells or monocytes (14, 15). CRISP-3 is released from neutrophil and eosinophil granules following cell stimulation (1, 15). Mature human CRISP-3 shares 48% and 65% amino acid (aa) sequence identity with mouse and equine CRISP-3, respectively. It shares 44% and 72% aa sequence identity with human CRISP-1 and -2, respectively.

  1. Kjeldsen, L. et al. (1996) FEBS Lett. 380:246.
  2. Kratzschmar, J. et al. (1996) Eur. J. Biochem. 236:827.
  3. Udby, L. et al. (2002) J. Immunol. Meth. 263:43.
  4. Yamazaki, Y. and Morita, T. (2004) Toxicon 44:227.
  5. Guo, M. et al. (2005) J. Biol. Chem. 280:12405.
  6. Haendler, B. et al. (1999) J. Cell. Physiol. 178:371.
  7. Udby, L. et al. (2005) J. Androl. 26:333.
  8. Haendler, B. et al. (1993) Endocrinology 133:192.
  9. Haendler, B. et al. (1997) Eur. J. Biochem. 250:440.
  10. Bjartell, A. et al. (2006) Prostate 66:591.
  11. Liao, Q. et al. (2003) Histol. Histopathol. 18:245.
  12. Udby, L. et al. (2005) Biochem. Biophys. Res. Commun. 333:555.
  13. Udby, L. et al. (2004) Biochemistry 43:12877.
  14. Pfisterer, P. et al. (1996) Mol. Cell. Biol. 16:6160.
  15. Udby, L. et al. (2002) J. Leukoc. Biol. 72:462.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

NBP2-02450
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
MAB4675
Species: Mu
Applications: WB, IHC, Neut
NBP1-76277
Species: Hu
Applications: WB, ELISA, ICC/IF, IHC, IHC-P, ICC
NBP2-15142
Species: Hu
Applications: WB, IHC, IHC-P
NBP1-92025
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
NB100-56603
Species: Hu, Pm
Applications: WB, IHC, IHC-P
NBP1-87217
Species: Hu, Mu
Applications: WB, IHC, IHC-P
NBP1-32920
Species: Hu, Mu
Applications: WB, ICC/IF, IHC, IHC-P
H00005324-M02
Species: Hu
Applications: WB, ELISA, IHC, IHC-P
H00005452-D01P
Species: Hu, Mu, Rt
Applications: WB
H00007001-M01
Species: Hu
Applications: WB, ELISA, ICC/IF, IHC-P
AF8537
Species: Mu, Rt
Applications: WB, Simple Western, ICC
NBP2-46321
Species: Hu
Applications: WB, IHC
AF755
Species: Mu
Applications: WB, Block

Publications for CRISP-3 Antibody (IC23972N) (0)

There are no publications for CRISP-3 Antibody (IC23972N).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for CRISP-3 Antibody (IC23972N) (0)

There are no reviews for CRISP-3 Antibody (IC23972N). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen
  • 1st to review with an image -- $50/€35/£30/$50 CAD/¥300 Yuan/¥5000 Yen

FAQs for CRISP-3 Antibody (IC23972N) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Additional Array Products

Bioinformatics Tool for CRISP-3 Antibody (IC23972N)

Discover related pathways, diseases and genes to CRISP-3 Antibody (IC23972N). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for CRISP-3 Antibody (IC23972N)

Discover more about diseases related to CRISP-3 Antibody (IC23972N).
 

Pathways for CRISP-3 Antibody (IC23972N)

View related products by pathway.

PTMs for CRISP-3 Antibody (IC23972N)

Learn more about PTMs related to CRISP-3 Antibody (IC23972N).

Blogs on CRISP-3

There are no specific blogs for CRISP-3, but you can read our latest blog posts.

Customers Who Bought This Also Bought

CRISP-3
2397-CR-050

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our CRISP-3 Antibody (295208) [Alexa Fluor® 700] and receive a gift card or discount.

Bioinformatics

Gene Symbol CRISP3
Uniprot