CRHBP Antibody (395108)

• Reactivity: Hu
• Applications: WB
• Clone: 395108
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

CRHBP Antibody (395108) Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human CRHBP
  Tyr25-Leu322
  Accession # P24387
• Specificity: Detects human CRHBP in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant mouse CRHBP is observed.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: CRHBP
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CRHBP Antibody (395108)

• corticotropin releasing hormone binding protein
• corticotropin-releasing factor-binding protein
• Corticotropin-releasing hormone-binding protein
• CRFBP
• CRFBPcorticotropin releasing hormone-binding protein
• CRF-BPCRF-binding protein
• CRHBP
• CRH-BP

Background

Human CRHBP is a 37 kDa secreted glycoprotein that likely serves to regulate corticotrophin releasing hormone (CRH) availability to the adrenal. In human, CRHBP complexes with CRH to form a circulating 41 kDa complex. In rodent and horse, this complex is restricted to the CNS. Human CRHBP is synthesized as a 322 amino acid (aa) precursor that contains a 24 aa signal sequence and a 298 aa mature region. The mature region contains one potential CUB domain and five intrachain disulfide bonds. Human mature CRHBP shares 87% and 84% sequence identity with mouse and equine CRHBP, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CRHBP Antibody (MAB2796)(1)

Publication using MAB2796

• A. Easter, E. Taborelli, A. Bye, P.A. Zunszain, C.M. Pariante, J. Treasure et al. Perinatal hypothalamic-pituitary-adrenal axis regulation among women with eating disorders and their infants Psychoneuroendocrinology 2017-02-01 [PMID: 27914245]

Bioinformatics

• Gene Symbol: CRHBP
• Uniprot:
  • P24387()