CREB Antibody

Product Discontinued

CREB Antibody Summary

• Immunogen: CREB peptide corresponding to a region near the N-terminus of the human protein, conjugated to Keyhole Limpet Hemocyanin (KLH).
• Specificity: Anti-CREB-1 (p43) may react non-specifically with other proteins. A partial cross-reactivity is observed against CREM-1 protein.
• Isotype: Serum
• Clonality: Polyclonal
• Host: Rabbit
• Gene: CREB1
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:5000-1:25000
  • Gel Super Shift Assays 0.5-1.0 ul per assay
  • Western Blot 1:500-1:1000
• Application Notes: This product was assayed against a lysate of 1 x 10^6 Raji B cells by immunoblot and found - be reactive against CREB-1 , showing a 46 kDa band at a dilution of 1:500 - 1:1,000 followed by reaction with Peroxidase conjugated Affinity Purified anti-Rabbit IgG [Goat] . Anti-CREB-1 is suitable for detection by immunoblot of human, mouse and rat CREB-1 . This product was also tested in a gel supershift assay and found - be reactive against CREB-1 using 0.5 - 1.0 ul per assay.

Reactivity Notes

CREB antibody may react non-specifically with other proteins. A partial cross-reactivity is observed against CREM-1 protein.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Preservative: 0.01% Sodium Azide
• Concentration: 2.56 mg/ml
• Purity: IgG purified

Alternate Names for CREB Antibody

• active transcription factor CREB
• cAMP responsive element binding protein 1
• cAMP-response element-binding protein-1
• cAMP-responsive element-binding protein 1
• CREB
• CREB1
• CREB-1
• cyclic AMP-responsive element-binding protein 1
• MGC9284
• transactivator protein

Background

CREB is a transcription factor which binds to cyclic AMP response elements (CRE's), and is induced by phosphoyrlation on the part of PKA, CaM K IV, and other protein kinases. Activated CREB will dimerize, bind to DNA, and recruit histone acetyl transferases.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

FAQs for CREB Antibody (NB600-489). (Showing 1 - 1 of 1 FAQ).

1. May I know which creb1 antibody is good for ChIP? I found a paper using your rabbit polyclonal creb1 antibody for ChIP but they didn't indicate the catalog number.
   • We are not aware of any of our Creb1 antibodies being used in ChIP at this time. If you can provide us with the PMID of the paper you are referring to I can contact the author. Otherwise, you can use our Innovators Reward Program to try an antibody in a novel species and application.

Bioinformatics

• Gene Symbol: CREB1
• Entrez:
  • 1385(Human)
  • 81646(Rat)