CREB [p Ser121] Antibody

Images

 
Western Blot: CREB [p Ser121] Antibody [NB100-410] - Detection of phospho-CREB in human HEK 293T cells. Top panel: probed with NB 100-410. Bottom panel: probed with CREB antibody. Lanes 2 and 4 treated with lambda ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.3 mg/ml
   

Biological Strategies

     

CREB [p Ser121] Antibody Summary

Immunogen
A phospho-peptide spanning the Ser121 phosphorylation site of human CREB [UniProt# P16220]
Modification
p Ser121
Localization
Nuclear
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
CREB1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500
  • Immunoprecipitation 0.5 ug
Application Notes
This CREB [p Ser121] antibody is useful for Immunoprecipitation and Western blot.
Positive Control
CREB Lysate (NBL1-09461)
Publications
Read Publications using
NB100-410 in the following applications:

  • WB
    3 publications

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Tris and 50% Glycerol
Preservative
0.05% Sodium Azide
Concentration
0.3 mg/ml
Purity
Immunogen affinity purified

Alternate Names for CREB [p Ser121] Antibody

  • active transcription factor CREB
  • cAMP responsive element binding protein 1
  • cAMP-response element-binding protein-1
  • cAMP-responsive element-binding protein 1
  • CREB
  • CREB1
  • CREB-1
  • cyclic AMP-responsive element-binding protein 1
  • MGC9284
  • transactivator protein

Background

CREB (cyclic-AMP response element binding) belongs to bZIP family of transcription factors which acts as a conduit between upstream signalling kinases and downstream target-gene transcription. Following Ser 133 phosphorylation mediated activation; CREB dimerizes for binding to CRE motifs on promoters containing consensus sequence TGACGTCA and together with its anchoring binding partner CBP, pCREB up-regulates transcription of target genes. CREB mediated transcription activation is also enhanced by transducers of regulated CREB (TORC) coactivators which act independently of Ser-133 phosphorylation. CREB's target genes include its family members (CREM, ICER and ATF) and these paralogs/homologs of CREB bind CRE elements to modulate the transcription of particular genes and CREB transcriptional regulation is involved in cell survival, plasticity, learning/memory paradigms, synchronization of circadian rhythmicity and adipose cells differentiation. Over-expression of CREB in transgenic animals can impart oncogenic properties on cells in various tissues and that aberrant CREB expression is associated with human malignancies. Moreover, defects in CREB1 have been linked to angiomatoid fibrous histiocytoma (AFH).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

NBP2-37576
Species: Hu, Mu, Pm
Applications: WB, ELISA, Flow, ICC/IF, IHC
NBP2-50037
Species: Hu, Mu, Rt, Po, Bv, Eq
Applications: WB, ICC/IF
NBP2-22203
Species: Hu, Mu, Rt, Mk
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-P
NB100-381
Species: Hu
Applications: WB, ChIP, IP, PLA
248-BD/CF
Species: Hu, Mu, Rt, Ca, Eq
AF467
Species: Hu, Mu
Applications: WB, Simple Western, Flow, IHC, CyTOF-ready, ICC
NB100-56530
Species: Hu
Applications: WB
MAB5526
Species: Hu, Mu
Applications: WB
NBP1-89544
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
NB100-56749
Species: Hu, Mu
Applications: WB, IHC, IHC-P
NBP2-29323
Species: Hu, Mu, Rt, Bv, Ca, Ch, Xp
AF1310
Species: Hu
Applications: WB, IHC, IP
NB500-487
Species: Hu, Mu, Rt, Bv
Applications: WB, IHC, IHC-P, IP
NB100-81898
Species: Hu, Po
Applications: WB, ICC/IF, IHC, IHC-P
NBP1-76729
Species: Hu
Applications: WB, ELISA, ICC/IF, IHC, IHC-P
H00005956-R02
Species: Hu
NB100-410
Species: Hu, Mu
Applications: WB, IP

Publications for CREB Antibody (NB100-410)(8)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: WB.


Filter By Application
WB
(3)
All Applications
Filter By Species
Human
(2)
All Species
Showing Publications 1 - 8 of 8.
Publications using NB100-410 Applications Species
Steven A, Leisz S, Sychra K et al. Hypoxia-mediated alterations and their role in the HER-2/neuregulated CREB status and localization Oncotarget Aug 9 2016 [PMID: 27409833] (WB, Human) WB Human
Byrd PJ, Stewart GS, Smith A et al. A Hypomorphic PALB2 Allele Gives Rise to an Unusual Form of FA-N Associated with Lymphoid Tumour Development. PLoS Genet 2016 Mar [PMID: 26990772] (WB) WB
Byrd PJ, Srinivasan V, Last JI et al. Severe reaction to radiotherapy for breast cancer as the presenting feature of ataxia telangiectasia. Br J Cancer. 2011 Dec 6. [PMID: 22146522]
Shi Y et al. Identification of carboxyl-terminal MCM3 phosphorylation sites using polyreactive phosphospecific antibodies. J Biol Chem;282 (12):9236-43. 2007 Mar 23. [PMID: 17244605]
Shanware NP et al. Coregulated ataxia telangiectasia-mutated and casein kinase sites modulate cAMP-response element-binding protein-coactivator interactions in response to DNA damage. J Biol Chem;282(9):6283-91. 2007 Mar 2. [PMID: 17209043]
Dodson GE, Tibbetts RS. DNA replication stress-induced phosphorylation of cyclic AMP response element-binding protein mediated by ATM. J Biol Chem;281(3):1692-7. 2006 Jan 20. [PMID: 16293623] (WB, Human) WB Human
Shi Y et al. Ataxia-telangiectasia-mutated (ATM) is a T-antigen kinase that controls SV40 viral replication in vivo. J Biol Chem;280(48):40195-200. 2005 Dec 2. [PMID: 16221684]
Shi Y et al. Direct regulation of CREB transcriptional activity by ATM in response to genotoxic stress. Proc Natl Acad Sci U S A;101(16):5898-903. 2004 Apr 20. [PMID: 15073328]

Reviews for CREB Antibody (NB100-410) (0)

There are no reviews for CREB Antibody (NB100-410). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen
  • 1st to review with an image -- $50/€35/£30/$50 CAD/¥300 Yuan/¥5000 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for CREB Antibody (NB100-410). (Showing 1 - 1 of 1 FAQs).

  1. May I know which creb1 antibody is good for ChIP? I found a paper using your rabbit polyclonal creb1 antibody for ChIP but they didn't indicate the catalog number.
    • We are not aware of any of our Creb1 antibodies being used in ChIP at this time. If you can provide us with the PMID of the paper you are referring to I can contact the author. Otherwise, you can use our Innovators Reward Program to try an antibody in a novel species and application.

Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NB100-410AF405
Alexa Fluor 488 Labeled NB100-410AF488
Alexa Fluor 647 Labeled NB100-410AF647
Alexa Fluor 700 Labeled NB100-410AF700
Biotin Labeled NB100-410B
DyLight 405 Labeled NB100-410V
DyLight 488 Labeled NB100-410G
DyLight 550 Labeled NB100-410R
DyLight 650 Labeled NB100-410C
HRP Labeled NB100-410H

Additional CREB Products

Bioinformatics Tool for CREB Antibody (NB100-410)

Discover related pathways, diseases and genes to CREB Antibody (NB100-410). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for CREB Antibody (NB100-410)

Discover more about diseases related to CREB Antibody (NB100-410).
 

Pathways for CREB Antibody (NB100-410)

View related products by pathway.

PTMs for CREB Antibody (NB100-410)

Learn more about PTMs related to CREB Antibody (NB100-410).
 

Research Areas for CREB Antibody (NB100-410)

Find related products by research area.

Blogs on CREB.

Exploring the Many Roles of PGC-1 alpha
The peroxisome proliferator-activated receptor gamma, co-activator 1 (PGC-1 alpha or PPARGC1A) gene encodes a 91 kDa nuclear protein that acts as a transcriptional co-activator involved in energy metabolism. Interaction with PPAR gamma allows it to in...  Read full blog post.

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our CREB [p Ser121] Antibody and receive a gift card or discount.

Bioinformatics

Gene Symbol CREB1
Entrez
Uniprot