CREB [p Ser121] Antibody

Western Blot: CREB [p Ser121] Antibody [NB100-410] - Detection of phospho-CREB in human HEK 293T cells. Top panel: probed with NB 100-410. Bottom panel: probed with CREB antibody. Lanes 2 and 4 treated with lambda phosphatase, lanes 3 and 4 treated with 0.2 mM H2O2.

• Reactivity: Hu, Mu
• Applications: WB, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 0.3 mg/ml

CREB [p Ser121] Antibody Summary

• Immunogen: A phospho-peptide spanning the Ser121 phosphorylation site of human CREB. [UniProt# P16220]
• Modification: p Ser121
• Localization: Nuclear
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: CREB1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:500
  • Immunoprecipitation 0.5 ug
• Control:

  CREB Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris, 50% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 0.3 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for CREB [p Ser121] Antibody

• active transcription factor CREB
• cAMP responsive element binding protein 1
• cAMP-response element-binding protein-1
• cAMP-responsive element-binding protein 1
• CREB
• CREB1
• CREB-1
• cyclic AMP-responsive element-binding protein 1
• MGC9284
• pCREB
• transactivator protein

Background

CREB (cyclic-AMP response element binding) belongs to bZIP family of transcription factors which acts as a conduit between upstream signalling kinases and downstream target-gene transcription. Following Ser 133 phosphorylation mediated activation; CREB dimerizes for binding to CRE motifs on promoters containing consensus sequence TGACGTCA and together with its anchoring binding partner CBP, pCREB up-regulates transcription of target genes. CREB mediated transcription activation is also enhanced by transducers of regulated CREB (TORC) coactivators which act independently of Ser-133 phosphorylation. CREB's target genes include its family members (CREM, ICER and ATF) and these paralogs/homologs of CREB bind CRE elements to modulate the transcription of particular genes and CREB transcriptional regulation is involved in cell survival, plasticity, learning/memory paradigms, synchronization of circadian rhythmicity and adipose cells differentiation. Over-expression of CREB in transgenic animals can impart oncogenic properties on cells in various tissues and that aberrant CREB expression is associated with human malignancies. Moreover, defects in CREB1 have been linked to angiomatoid fibrous histiocytoma (AFH).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CREB Antibody (NB100-410)(8)

Publications using NB100-410

• Steven A, Leisz S, Sychra K et al. Hypoxia-mediated alterations and their role in the HER-2/neuregulated CREB status and localization Oncotarget Aug 9 2016 [PMID: 27409833] (WB, Human)
• Byrd PJ, Stewart GS, Smith A et al. A Hypomorphic PALB2 Allele Gives Rise to an Unusual Form of FA-N Associated with Lymphoid Tumour Development. PLoS Genet 2016 Mar [PMID: 26990772] (WB)
• Byrd PJ, Srinivasan V, Last JI et al. Severe reaction to radiotherapy for breast cancer as the presenting feature of ataxia telangiectasia. Br J Cancer. 2011 Dec 6. [PMID: 22146522]
• Shi Y et al. Identification of carboxyl-terminal MCM3 phosphorylation sites using polyreactive phosphospecific antibodies. J Biol Chem;282 (12):9236-43. 2007 Mar 23. [PMID: 17244605]
• Shanware NP et al. Coregulated ataxia telangiectasia-mutated and casein kinase sites modulate cAMP-response element-binding protein-coactivator interactions in response to DNA damage. J Biol Chem;282(9):6283-91. 2007 Mar 2. [PMID: 17209043]
• Dodson GE, Tibbetts RS. DNA replication stress-induced phosphorylation of cyclic AMP response element-binding protein mediated by ATM. J Biol Chem;281(3):1692-7. 2006 Jan 20. [PMID: 16293623] (WB, Human)
• Shi Y et al. Ataxia-telangiectasia-mutated (ATM) is a T-antigen kinase that controls SV40 viral replication in vivo. J Biol Chem;280(48):40195-200. 2005 Dec 2. [PMID: 16221684]
• Shi Y et al. Direct regulation of CREB transcriptional activity by ATM in response to genotoxic stress. Proc Natl Acad Sci U S A;101(16):5898-903. 2004 Apr 20. [PMID: 15073328]

FAQs for CREB Antibody (NB100-410). (Showing 1 - 1 of 1 FAQs).

1. May I know which creb1 antibody is good for ChIP? I found a paper using your rabbit polyclonal creb1 antibody for ChIP but they didn't indicate the catalog number.
   • We are not aware of any of our Creb1 antibodies being used in ChIP at this time. If you can provide us with the PMID of the paper you are referring to I can contact the author. Otherwise, you can use our Innovators Reward Program to try an antibody in a novel species and application.

Bioinformatics

• Gene Symbol: CREB1
• Entrez:
  • 1385(Human)
  • 12912(Mouse)
• Uniprot:
  • P16220()