CREB Antibody (SA04-04)

Western Blot: CREB Antibody (SA04-04) [NBP2-67274] - Western blot analysis of CREB on zebrafish tissue lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat An

Immunocytochemistry/Immunofluorescence: CREB Antibody (SA04-04) [NBP2-67274] - Staining CREB in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: CREB Antibody (SA04-04) [NBP2-67274] - Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-CREB antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30

Flow Cytometry: CREB Antibody (SA04-04) [NBP2-67274] - Analysis of Hela cells with CREB antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.

Western Blot: CREB Antibody (SA04-04) [NBP2-67274] - Analysis of CREB on different cell lysates using anti-CREB antibody at 1/1,000 dilution. Positive control: Lane 1: Hela Lane 2: HepG2 Lane 3: Jurkat Lane 4: NIH/3T3

Immunohistochemistry-Paraffin: CREB Antibody (SA04-04) [NBP2-67274] - Analysis of paraffin-embedded human breast carcinoma tissue using anti-CREB antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: CREB Antibody (SA04-04) [NBP2-67274] - Analysis of paraffin-embedded human lung cancer tissue using anti-CREB antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: CREB Antibody (SA04-04) [NBP2-67274] - Analysis of paraffin-embedded human tonsil tissue using anti-CREB antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: CREB Antibody (SA04-04) [NBP2-67274] - Analysis of paraffin-embedded mouse brain tissue using anti-CREB antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: CREB Antibody (SA04-04) [NBP2-67274] - Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-CREB antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5%

Immunohistochemistry-Paraffin: CREB Antibody (SA04-04) [NBP2-67274] - Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using anti-CREB antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 5% BSA

Immunohistochemistry-Paraffin: CREB Antibody (SA04-04) [NBP2-67274] - Immunohistochemical analysis of paraffin-embedded human pancreas tissue using anti-CREB antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for

Immunohistochemistry-Paraffin: CREB Antibody (SA04-04) [NBP2-67274] - Immunohistochemical analysis of paraffin-embedded human thyroid tissue using anti-CREB antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 3

Immunohistochemistry-Paraffin: CREB Antibody (SA04-04) [NBP2-67274] - Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-CREB antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 5% BSA for 30

Product Discontinued

CREB Antibody (SA04-04) Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Synthetic peptide within human CREB aa 250-290. (SwissProt: P16220 Human; SwissProt: Q01147 Mouse; SwissProt: P15337 Rat)
• Localization: Nucleus
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: CREB1
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:50-1:100
  • Immunocytochemistry/ Immunofluorescence 1:50-1:200
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50-1:500
  • Immunoprecipitation
  • Western Blot 1:500-1:5000
• Application Notes: IP-assay-dependent

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: TBS (pH7.4), 0.05% BSA, 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for CREB Antibody (SA04-04)

• active transcription factor CREB
• cAMP responsive element binding protein 1
• cAMP-response element-binding protein-1
• cAMP-responsive element-binding protein 1
• CREB
• CREB1
• CREB-1
• cyclic AMP-responsive element-binding protein 1
• MGC9284
• transactivator protein

Background

CREB (cAMP responsive element binding protein 1) stimulates transcription. CREB is involved in learning and memory. This protein is known to have interactions with HIST1H3B, HIST1h3A, HIST1H3C, HIST1H3D and HIST1H3E. CREB has been studied in relation to several diseases and disorders including Rubinstein-Taybi syndrome, Alzheimer's disease, leukemia and brain disease.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

FAQs for CREB Antibody (NBP2-67274). (Showing 1 - 1 of 1 FAQ).

1. May I know which creb1 antibody is good for ChIP? I found a paper using your rabbit polyclonal creb1 antibody for ChIP but they didn't indicate the catalog number.
   • We are not aware of any of our Creb1 antibodies being used in ChIP at this time. If you can provide us with the PMID of the paper you are referring to I can contact the author. Otherwise, you can use our Innovators Reward Program to try an antibody in a novel species and application.

Bioinformatics

• Gene Symbol: CREB1