COX6A1 Antibody (OTI4G2)

Western Blot: COX6A1 Antibody (4G2) [NBP2-02781] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY COX6A1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-COX6A1.

Immunocytochemistry/Immunofluorescence: COX6A1 Antibody (4G2) [NBP2-02781] Staining of COS7 cells transiently transfected by pCMV6-ENTRY COX6A1.

Flow Cytometry: COX6A1 Antibody (4G2) [NBP2-02781] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-COX6A1 antibody, and then analyzed by flow cytometry.

• Reactivity: Hu
• Applications: WB, Flow, ICC/IF
• Clone: OTI4G2
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 0.52 mg/ml

COX6A1 Antibody (OTI4G2) Summary

• Immunogen: Full length human recombinant protein of human COX6A1 (NP_004364) produced in HEK293T cell.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: COX6A1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:100
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Western Blot 1:500
• Theoretical MW: 9.6 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 1.0% BSA and 50% Glycerol
• Preservative: 0.02% Sodium Azide
• Concentration: 0.52 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for COX6A1 Antibody (OTI4G2)

• COX VIa-L
• COX6A
• COX6AL
• Cytochrome c oxidase polypeptide VIa-liver
• cytochrome c oxidase subunit 6A1, mitochondrial
• cytochrome C oxidase subunit VIa homolog
• cytochrome c oxidase subunit VIa polypeptide 1
• Cytochrome c oxidase subunit VIA-liver
• MGC104500

Background

Cytochrome c oxidase (COX), the terminal enzyme of the mitochondrial respiratory chain, catalyzes the electron transfer from reduced cytochrome c to oxygen. It is a heteromeric complex consisting of 3 catalytic subunits encoded by mitochondrial genes and multiple structural subunits encoded by nuclear genes. The mitochondrially-encoded subunits function in the electron transfer and the nuclear-encoded subunits may function in the regulation and assembly of the complex. This nuclear gene encodes polypeptide 1 (liver isoform) of subunit VIa, and polypeptide 1 is found in all non-muscle tissues. Polypeptide 2 (heart/muscle isoform) of subunit VIa is encoded by a different gene, and is present only in striated muscles. These two polypeptides share 66% amino acid sequence identity. It has been reported that there may be several pseudogenes on chromosomes 1, 6, 7q21, 7q31-32 and 12. However, only one pseudogene (COX6A1P) on chromosome 1p31.1 has been documented. [provided by RefSeq].

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: COX6A1
• Entrez:
  • 1337(Human)