COX-1 Antibody

Western Blot: COX-1 Antibody [24300002] - Western Blot was performed using affinity purified SEQer PTGS1 antibody. The lanes contain 5-30ug of a whole cell extract. Final concentration of antibodies = 0.1ug/ml (1:10,000 dilution).

ELISA: COX-1 Antibody [24300002] - Affinity Purified

Product Discontinued

COX-1 Antibody Summary

• Immunogen: This antibody is specific for the Middle Region of the target protein.
• Specificity: This product is specific for Human PTGS1.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: PTGS1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:250-1:2000
  • Western Blot 1:5000-1:20000
• Application Notes: This antibody is useful in ELISA, Western Blot and Immunohistochemistry-Paraffin.

Reactivity Notes

Human.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 20mM Potassium Phosphate (pH 7.0) and 0.15M NaCl
• Preservative: No Preservative
• Purity: Immunogen affinity purified

Notes

Manufactured by SDIX's proprietary Genomic Antibody Technology™. GAT FAQs.

Alternate Names for COX-1 Antibody

• COX1
• COX-1
• COX1PGG/HS
• COX3
• cyclooxygenase-1
• EC 1.14.99.1
• PCOX1
• PGH synthase 1
• PGHS1
• PGHS-1PHS1
• PHS 1
• prostaglandin G/H synthase 1
• prostaglandin G/H synthase and cyclooxygenase
• Prostaglandin H2 synthase 1
• prostaglandin-endoperoxide synthase 1 (prostaglandin G/H synthase andcyclooxygenase)
• Prostaglandin-endoperoxide synthase 1
• PTGHS
• PTGS1

Background

Cyclooxygenase (COX), also known as prostaglandin H2 synthese and prostaglandin endoperoxide synthase, is an important enzyme in the conversion of arachidonic acid to prostaglandin H2.1, 2 Prostaglandin H2 is converted by other enzymes into inflammatory mediators such as prostaglandin (PG) D2, PGE2, PGF2a, PG12, and thromboxane A2. Thus, COX is a key enzyme in the production of inflammatory agents and is the target of intense research and drug discovery activities. COX consists of two isoforms, COX-I (599 amino acid residues) and COX-II (604 amino acid residues). The COX enzymes, membrane-associated heme proteins that have cyclooxygenase and peroxidase activities, are targets of NSAID (nonsteroidal anti-inflammatory drugs) such as aspirin.3 COX-I is involved in the regulation of homeostatic functions throughout the body, such as vascular hemostasis, renal blood flow, and maintenance of glomerular function.4, 5, 6 It is constitutively produced in most cell types with high expression levels found in gastrointestinal tissues. COX-I is important in the gut for the production of prostaglandins, which inhibit gastric secretion. COX-I can be induced in monocytes, macrophages, and other cells as part of the inflammatory response by IL-1b and other cytokines. It is also induced by growth factors such as EGF and PDGF. Expression is inhibited by glucocorticosteroids such as cortisol and dexamethasone.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for COX-1 Antibody (24300002)(2)

Publications using 24300002

• Garrison C, Lastwika K, Zhang Y et al. Proteomic Analysis, Immune Dysregulation, and Pathway Interconnections With Obesity J Proteome Res. 2017-01-06 [PMID: 27769113] (MiAr)
  
  Details:
  Analysis is performed on plasma proteomic data to identify how obesity can alter pathways and to highlight the risk factor for disease in subjects with a high body mass index.
• Rho JH, Lampe PD. High-throughput screening for native autoantigen-autoantibody complexes using antibody microarrays J Proteome Res. 2013-05-03 [PMID: 23541305] (MiAr)
  
  Details:
  A novel method using antibody microarrays is used to detect autoantibody-antigen complexes that can potentially be useful for detection and characterization of diseases.

FAQs for COX-1 Antibody (24300002). (Showing 1 - 1 of 1 FAQs).

1. Please could you tell me if Novus Biologicals has an active COX1 Recombinant Protein?
   • Unfortunately we do not currently have any COX1 recombinant proteins that are expected to be active.

Bioinformatics

• Gene Symbol: PTGS1
• Entrez:
  • 5742(Human)
• Uniprot:
  • P23219()