Contactin-6 Antibody [Alexa Fluor™ Plus 555]

• Reactivity: Mu
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Alexa Fluor Plus 555

Contactin-6 Antibody [Alexa Fluor™ Plus 555] Summary

• Specificity: Detects mouse Contactin-6 in direct ELISAs and Western blots. In direct ELISAs, approximately 50% cross-reactivity with recombinant human (rh) Contactin‑6 is observed, and less than 5% cross-reactivity with recombinant mouse (rm) Contactin-3, rmContactin-4, rmContactin‑5, and rhTAG-1 is observed.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Contactin-6 Antibody [Alexa Fluor™ Plus 555]

• CNTN6
• contactin 6
• Contactin6
• Contactin-6
• MGC133256
• NB3

Background

Contactin-6, also known as NB-3, is a 130 kDa member of the TAG/F3 subgroup of Contactin GPI-linked neuronal adhesion proteins (1, 2). Mature mouse Contactin-6 consists of six immunoglobulin-like domains, a flexible linker region, four fibronectin type III domains, and a GPI anchor (3-5). Mouse Contactin-6 shares 90% and 96% amino acid (aa) sequence identity with human and rat Contactin-6, respectively. Alternate splicing generates an isoform with a 17 aa deletion in the first Ig-like domain (3). Contactin-6 expression is upregulated postnatally in the cerebellum, brainstem, and hippocampus, and it shows differential expression within various structures of the brain (3, 5-7). In the cerebellum, it is presynaptically localized at synapses between excitatory glutamatergic parallel fibers and Purkinje cells (7). Contactin-6 associates in cis with CHL-1 and in trans with Notch-1 on oligodendrocytes (6, 8). Its binding to Notch-1 triggers translocation of the Notch intracellular domain to the nucleus and promotes oligodendrogenesis (6). Contactin-6 function is important in the postnatal development and function of the cerebellum, as shown in knockout mice which exhibit poor motor coordination (5, 7).

1. Shimoda, Y. and K. Watanabe (2009) Cell Adhesion Migration 3:64.
2. Hu, Q.D. et al. (2006) Dev. Neurosci. 28:25.
3. Lee, S. et al. (2000) Gene 245:253.
4. Ogawa, J. et al. (1996) Neurosci. Lett. 218:173.
5. Takeda, Y. et al. (2003) J. Neurobiol. 56:252.
6. Cui, X.-Y. et al. (2004) J. Biol. Chem. 279:25858.
7. Sakurai, K. et al. (2009) Dev. Neurobiol. Aug 11 epub.
8. Ye, H. et al. (2008) EMBO J. 27:188.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.