Contactin-3 Antibody

Contactin-3 was detected in immersion fixed paraffin-embedded sections of human brain (cerebellum) using Sheep Anti-Human Contactin-3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7125) at 3 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to Purkinje neurons. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

• Reactivity: Hu
• Applications: IHC
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Unconjugated
• Concentration: LYOPH

Contactin-3 Antibody Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human Contactin-3
  Pro26-Pro992
  Accession # Q9P232
• Specificity: Detects human Contactin-3 in direct ELISAs. In direct ELISAs, approximately 10% cross-reactivity with recombinant mouse Contactin-3 is observed, and less than 5% cross-reactivity with recombinant human (rh) Contactin-4 and rhContactin-5 is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Gene: CNTN3
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 5-15 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Sterile PBS to a final concentration of 0.2 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Contactin-3 Antibody

• BIG-1
• CNTN3
• contactin 3 (plasmacytoma associated)
• Contactin3
• Contactin-3
• PANG
• PCS

Background

Contactin-3, also known as BIG-1, CNTN3 and PANG, is a 150 kDa member of the TAG/F3 subgroup of Contactin neuronal adhesion proteins (1, 2). Mature human Contactin-3 consists of six immunoglobulin-like domains, a flexible linker region, four fibronectin type III domains, and a GPI anchor (3, 4). Human Contactin-3 shares 92% aa sequence identity with mouse and rat Contactin-3. Contactin-3 is primarily expressed in the brain in discrete regions including the frontal lobe, occipital lobe, cerebellum, amygdala, and hippocampus (5, 6). Alternative splicing in rat may generate a soluble isoform (4). Mouse plasmacytoma cell lines produce multiple transcripts of Contactin-3 due to the presence of IAP proviruses (3). Immobilized Contactin-3 promotes neurite outgrowth from rat hippocampal neurons (4).

1. Shimoda, Y. and K. Watanabe (2009) Cell Adhesion Migration 3:64.
2. Denisenko-Nehrbass, N. et al. (2002) J. Physiol. 96:99.
3. Connelly, M.A. et al. (1994) Proc. Natl. Acad. Sci. 91:1337.
4. Yoshihara, Y. et al. (1994) Neuron 13:415.
5. Kamei, Y. et al. (2000) Genomics 69:113.
6. Yoshihara, Y. et al. (1995) J. Neurobiol. 28:51.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CNTN3
• Uniprot:
  • Q9P232()