Connexin 43/GJA1 Antibody

Western Blot: Connexin 43/GJA1 Antibody [NBP1-51938] - NBP1-51938 (0.3ug/ml) staining of Rat Brain lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

• Reactivity: Rt, Hu, Po, Ca
• Applications: WB, ELISA
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

Connexin 43/GJA1 Antibody Summary

• Description: Novus Biologicals Goat Connexin 43/GJA1 Antibody (NBP1-51938) is a polyclonal antibody validated for use in WB, ELISA and ICC/IF. Anti-Connexin 43/GJA1 Antibody: Cited in 2 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Peptide with sequence C-QPFDFPDDNQNSKK corresponding to internal region according to NP_000156.1.
• Epitope: C-QPFDFPDDNQNSKK
• Marker: Gap Junctions Marker
• Predicted Species: Human (100%), Porcine (100%), Canine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: GJA1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Peptide ELISA Detection limit 1:64000
  • Western Blot 1:100-1:2000
• Application Notes: WB: Approx. 40 kDa band observed in rat brain lysates (calculated MW of 43.0 kDa band according to human NP_000156.1 and to rat NP_036699.1).

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Connexin 43/GJA1 Antibody

• Connexin 43
• connexin-43
• CX43
• CX43gap junction protein, alpha-like
• DFNB38
• Gap junction 43 kDa heart protein
• gap junction alpha-1 protein
• gap junction protein, alpha 1, 43kDa (connexin 43)
• gap junction protein, alpha 1, 43kDa
• GJA1
• GJAL
• HSS
• ODD
• ODDD
• ODOD
• SDTY3

Background

The connexins are a group of GAP junction proteins, which form a hexamer to compose a connexon. Clusters of connexons form a GAP junction through which low molecular weight proteins may diffuse from cell to cell. Several mammalian cells with malignant phenotypes exhibit decreased connexin expression and GAP junction communication. There is a decrease in GAP junctional communication in Src transformed cells. The decreased communication appears to be associated with tyrosine phosphorylation of connexin 43. Activated c-Src phosphorylates the C-terminal tail of connexin 43 on residue Tyr 265, resulting in a stable interaction between both proteins leading to inhibition of GAP junctional communication. In addition to tyrosine phosphorylation, connexin 43 has also been shown to be phosphorylated on serine in the absence of Src kinases and on both serine and tyrosine in cells expressing Src kinases such as pp60v-Src and/or c-Src.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Connexin 43/GJA1 Antibody (NBP1-51938)(2)

Publications using NBP1-51938

• Fossey MPM, Balthazaar SJT, Squair JW et al. Spinal cord injury impairs cardiac function due to impaired bulbospinal sympathetic control Nature communications 2022-03-16 [PMID: 35296681] (ICC/IF, Rat)
• de la Parra DR, Zenteno JC. A new GJA1 (connexin 43) mutation causing oculodentodigital dysplasia associated to uncommon features. Ophthalmic Genet 2007-12-01 [PMID: 18161618]

Bioinformatics

• Gene Symbol: GJA1
• Entrez:
  • 2697(Human)
  • 24392(Rat)
• Uniprot:
  • NP_000156.1(Human)