Connexin 43/GJA1 Antibody


Western Blot: Connexin 43/GJA1 Antibody [NBP1-51938] - NBP1-51938 (0.3ug/ml) staining of Rat Brain lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Product Details

Reactivity RtSpecies Glossary
Applications WB, PEP-ELISA
0.5 mg/ml

Order Details

Connexin 43/GJA1 Antibody Summary

Peptide with sequence C-QPFDFPDDNQNSKK corresponding to internal region according to NP_000156.1.
Gap Junctions Marker
Immunogen affinity purified
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Packaging, Storage & Formulations

Store at -20C. Avoid freeze-thaw cycles.
Tris saline, pH 7.3 with 0.5% BSA.
0.02% Sodium Azide
0.5 mg/ml
Immunogen affinity purified


  • Western Blot 0.3 - 1 ug/ml
  • Peptide ELISA Detection limit 1:64000
Application Notes
WB: Approx. 40 kDa band observed in rat brain lysates (calculated MW of 43.0 kDa band according to human NP_000156.1 and to rat NP_036699.1).
Read Publication using NBP1-51938.

Reactivity Notes

Predicted cross-reactivity based on sequence identity: Human, Canine, Porcine.

Alternate Names for Connexin 43/GJA1 Antibody

  • connexin 43
  • connexin-43
  • Cx43
  • CX43gap junction protein, alpha-like
  • DFNB38
  • Gap junction 43 kDa heart protein
  • gap junction alpha-1 protein
  • gap junction protein, alpha 1, 43kDa (connexin 43)
  • gap junction protein, alpha 1, 43kDa
  • GJA1
  • GJAL
  • HSS
  • ODD
  • ODDD
  • ODOD
  • SDTY3


The connexins are a group of GAP junction proteins, which form a hexamer to compose a connexon. Clusters of connexons form a GAP junction through which low molecular weight proteins may diffuse from cell to cell. Several mammalian cells with malignant phenotypes exhibit decreased connexin expression and GAP junction communication. There is a decrease in GAP junctional communication in Src transformed cells. The decreased communication appears to be associated with tyrosine phosphorylation of connexin 43. Activated c-Src phosphorylates the C-terminal tail of connexin 43 on residue Tyr 265, resulting in a stable interaction between both proteins leading to inhibition of GAP junctional communication. In addition to tyrosine phosphorylation, connexin 43 has also been shown to be phosphorylated on serine in the absence of Src kinases and on both serine and tyrosine in cells expressing Src kinases such as pp60v-Src and/or c-Src.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Connexin 43/GJA1 Antibody (NBP1-51938)(1)

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Video Protocols

WB Video Protocol

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Secondary Antibodies


Isotype Controls

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Blogs on Connexin 43/GJA1.

Connexin: Bridging the Gap of Intercellular Communication
Connexin 43/GJA1 is a member of the connexin gene family and the most abundant protein component found within gap junctions. Gap junctions are the cell-to-cell contacts that provide direct intercellular communication between cells by regulating back a...  Read full blog post.

Contact Information

Product PDFs


Gene Symbol GJA1

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