Connexin 43/GJA1 Antibody (578618) [Allophycocyanin]

• Applications: Flow
• Clone: 578618
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Allophycocyanin

Connexin 43/GJA1 Antibody (578618) [Allophycocyanin] Summary

• Immunogen: Mouse myeloma cell line NS0 transfected with human Connexin 43/GJA1
  Accession # P17302
• Specificity: Detects human Connexin 43/GJA1 in direct ELISAs.
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: GJA1
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.
• Buffer: Supplied in a saline solution containing BSA and Sodium Azide.
• Preservative: Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Background

Connexin43 (Cx43; also gap junction alpha -1 protein) is a 41 - 44 kDa member of the connexin family, alpha -type subfamily, of transmembrane proteins. It is the most common type of connexin in cardiac muscle cells, and also occurs in hepatocytes, astrocytes and ovary granulosa cells. It is a 4-transmembrane protein 382 amino acids (aa) in length that contains two cytoplasmic tails. One is 12 aa in length at the N-terminus and the second is 151 aa in length at the C-terminus. Human and rat Cx43 are 98% aa identical over the entire length of the molecule. Connexins form gap junctions (GJs) which are intercellular channels between cells. Each adjacent cell contributes to a functional channel. The fundamental unit is a “connexon”, or hemi-channel, which is composed of six connexins in a sliding subunit hexamer configuration. The hexamer may be either homomeric or heteromeric. The connexon arrangement provides for the opening and closing of an intersubunit space (or pore) that allows diffusion of molecules 1 kDa or less. It is suggested that the extended C-terminus of each connexin may interact with multiple docking proteins and serve as a plug during closure. Connexons on adjacent cells interact via their extracellular loops to form a GJ channel. When open, these channels allow for the transit of small hydrophilic molecules such as ATP, glucose, IP3 and Ca++. Connexons (or hemi-channels) that do not interact with adjacent cells are also suggested to allow for small molecule transit under unusual circumstances. Cx43 activity is regulated by phosphorylation. The exact effects, however, may be context-specific. In one case, PKC phosphorylation of S368 has been found to decrease GJ channel permeability to select small molecular weight solutes. Alternatively, PKA has been suggested to be the mediator of Cx43 phosphorylation of S365, S368, S369 and S373. In this case, phosphorylation promotes channel activity.

1. Sohl, G. and K. Willecke (2004) Cardiovasc. Res. 62:228.
2. Willecke, K. et al. (2002) Biol. Chem. 383:725.
3. Giepmans, B.N.G. (2004) Cardiovasc. Res. 62:233.
4. Vinken, M. et al. (2006) Cell. Signal. 18:592.
5. Stout, C. et al. (2004) Curr. Opin. Cell Biol. 16:507.
6. Bao, X. et al. (2004) Am. J. Physiol. Cell. Physiol. 286:C647.
7. Yogo, K. et al. (2006) J. Reprod. Dev. 52:321.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: GJA1