Complexin-1 Antibody [Alexa Fluor® 405]

• Reactivity: Hu, Mu, Rt
• Applications: WB, IHC, ICC/IF
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Alexa Fluor 405

Complexin-1 Antibody [Alexa Fluor® 405] Summary

• Immunogen: E. coli-derived recombinant human Complexin-1
  Met1-Lys134
  Accession # O14810
• Specificity: Detects human, mouse, and rat Complexin-1 in direct ELISAs and Western blots.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry
  • Immunohistochemistry
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Complexin-1 Antibody [Alexa Fluor® 405]

• complexin 1
• Complexin I
• Complexin-1
• CPLX1
• CPX I
• CPX1
• CPX-I
• synaphin 2
• Synaphin-2

Background

Complexin-1 (CPX I; also synaphin-2) is a 17-18 kDa, cytosolic protein that belongs to the complexin/synaphin family, complexin-1/-2 subfamily of molecules. It is expressed in select neurons and pancreatic beta -cells, and serves as a block against premature neurotransmitter/insulin granule release. Both Complexin-1 and Complexin-2 bind to the SNARE complex that contains SNAP25, VAMP2 and STX1A, generating oligomers of SNARE complexes that are optimally oriented relative to the positions of vesicle and plasma membranes. This effectively places synaptic vesicles in an optimal configuration, allowing for a coordinated, uniform fusion and release upon appropriate stimulus. Typically, complexin-1 is described as a clamp that blocks (vesicle) fusion, and the stimulus that overcomes it is one that induces an influx of synaptotagmin-detectable calcium. Human complexin-1 is 134 amino acids (aa) in length. It contains one coiled-coil domain (aa 29-69) that incorporates part of a SNARE complex interaction sequence (aa 48-70). Full-length human complexin-1 shares 97% aa sequence identity with mouse complexin-1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.